(Circular) (Six-base) MAPSORT of: pF1KB8315.seq Check: 2119 from: 1 to: 3875 pF1KB8315 3875 bp With 182 enzymes: SgfI * June 20, 2008 10:28 .. AccI GT'mk_AC Cuts at: 913 913 Size: 3875 Acc65I G'GTAC_C Cuts at: 891 891 Size: 3875 AclI AA'CG_TT Cuts at: 3715 3715 Size: 3875 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 557 872 1700 2132 2879 3147 557 Size: 315 828 432 747 268 1285 Fragments arranged by size: 1285 828 747 432 315 268 AflIII A'CryG_T Cuts at: 1227 2331 3308 1227 Size: 1104 977 1794 Fragments arranged by size: 1794 1104 977 AgeI A'CCGG_T Cuts at: 2263 2263 Size: 3875 AlwNI CAG_nnn'CTG Cuts at: 487 795 1180 2747 487 Size: 308 385 1567 1615 Fragments arranged by size: 1615 1567 385 308 ApaLI G'TGCA_C Cuts at: 2645 2645 Size: 3875 ApoI r'AATT_y Cuts at: 300 879 3228 3484 300 Size: 579 2349 256 691 Fragments arranged by size: 2349 691 579 256 AseI AT'TA_AT Cuts at: 20 20 Size: 3875 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 3875 AvaI C'yCGr_G Cuts at: 430 895 430 Size: 465 3410 BamHI G'GATC_C Cuts at: 900 900 Size: 3875 BanI G'GyrC_C Cuts at: 891 1305 1553 1588 891 Size: 414 248 35 3178 Fragments arranged by size: 3178 414 248 35 BanII G_rGCy'C Cuts at: 431 634 889 1919 431 Size: 203 255 1030 2387 Fragments arranged by size: 2387 1030 255 203 BbeI G_GCGC'C Cuts at: 1309 1557 1309 Size: 248 3627 BbsI GAAGACnn'nnnn_ Cuts at: 521 521 Size: 3875 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 110 144 849 883 110 Size: 34 705 34 3102 Fragments arranged by size: 3102 705 34 34 BciVI GTATCCnnnnn_n' Cuts at: 126 1773 2534 3875 126 Size: 1647 761 1341 126 Fragments arranged by size: 1647 1341 761 126 BclI T'GATC_A Cuts at: 1395 1395 Size: 3875 BglII A'GATC_T Cuts at: 123 218 1390 3427 123 Size: 95 1172 2037 571 Fragments arranged by size: 2037 1172 571 95 BlpI GC'TnA_GC Cuts at: 987 987 Size: 3875 Bme1580I G_kGCm'C Cuts at: 1500 1593 2649 1500 Size: 93 1056 2726 Fragments arranged by size: 2726 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 1087 1168 1503 3647 1087 Size: 81 335 2144 1315 Fragments arranged by size: 2144 1315 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2242 2242 Size: 3875 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 609 609 Size: 3875 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1051 1930 2422 2720 1051 Size: 879 492 298 2206 Fragments arranged by size: 2206 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 226 3579 226 Size: 3353 522 BsaAI yAC'GTr Cuts at: 1228 1228 Size: 3875 BsaBI GATnn'nnATC Cuts at: 6 518 1389 3426 6 Size: 512 871 2037 455 Fragments arranged by size: 2037 871 512 455 BsaHI Gr'CG_yC Cuts at: 1306 1554 2256 3752 1306 Size: 248 702 1496 1429 Fragments arranged by size: 1496 1429 702 248 BsaWI w'CCGG_w Cuts at: 1064 1585 2263 2537 2684 3095 1064 Size: 521 678 274 147 411 1844 Fragments arranged by size: 1844 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 418 448 418 Size: 30 3845 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 267 556 654 675 267 Size: 289 98 21 3467 Fragments arranged by size: 3467 289 98 21 BseYI C'CCAG_C Cuts at: 259 751 756 1300 2058 2635 3131 259 Size: 492 5 544 758 577 496 1003 Fragments arranged by size: 1003 758 577 544 496 492 5 BsiEI CG_ry'CG Cuts at: 104 576 1417 1463 2671 104 Size: 472 841 46 1208 1308 Fragments arranged by size: 1308 1208 841 472 46 BsiHKAI G_wGCw'C Cuts at: 634 744 889 1667 1857 2649 634 Size: 110 145 778 190 792 1860 Fragments arranged by size: 1860 792 778 190 145 110 Bsp1286I G_dGCh'C Cuts at: 431 634 744 889 1500 1593 1667 1857 1919 Size: 203 110 145 611 93 74 190 62 Cuts at: 1919 2649 431 Size: 730 1657 Fragments arranged by size: 1657 730 611 203 190 145 110 93 74 62 BspEI T'CCGG_A Cuts at: 3095 3095 Size: 3875 BspHI T'CATG_A Cuts at: 3051 3051 Size: 3875 BspMI ACCTGCnnnn'nnnn_ Cuts at: 925 1139 1441 1822 925 Size: 214 302 381 2978 Fragments arranged by size: 2978 381 302 214 BsrBI CCG'CTC Cuts at: 50 780 2167 2221 3705 3870 50 Size: 730 1387 54 1484 165 55 Fragments arranged by size: 1484 1387 730 165 55 54 BsrDI GCAATG_nn' Cuts at: 1787 1787 Size: 3875 BsrFI r'CCGG_y Cuts at: 158 439 1873 2054 2263 158 Size: 281 1434 181 209 1770 Fragments arranged by size: 1770 1434 281 209 181 BssSI C'ACGA_G Cuts at: 2146 2504 2146 Size: 358 3517 BstAPI GCAn_nnn'nTGC Cuts at: 1252 1252 Size: 3875 BstBI TT'CG_AA Cuts at: 2236 2236 Size: 3875 BstYI r'GATC_y Cuts at: 1 123 218 900 1367 1390 1725 1971 2983 Size: 122 95 682 467 23 335 246 1012 Cuts at: 2983 3069 3081 3427 1 Size: 86 12 346 449 Fragments arranged by size: 1012 682 467 449 346 335 246 122 95 86 23 12 BtgI C'CryG_G Cuts at: 107 107 Size: 3875 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1825 2006 2169 1825 Size: 181 163 3531 Fragments arranged by size: 3531 181 163 BtsI GCAGTG_nn' Cuts at: 1118 1118 Size: 3875 DraI TTT'AAA Cuts at: 874 874 Size: 3875 DraIII CAC_nnn'GTG Cuts at: 507 507 Size: 3875 DrdI GACnn_nn'nnGTC Cuts at: 1581 2439 1581 Size: 858 3017 EaeI y'GGCC_r Cuts at: 573 1460 1634 2025 2052 3397 573 Size: 887 174 391 27 1345 1051 Fragments arranged by size: 1345 1051 887 391 174 27 EagI C'GGCC_G Cuts at: 573 1460 573 Size: 887 2988 EarI CTCTTCn'nnn_ Cuts at: 389 533 705 829 1898 2108 389 Size: 144 172 124 1069 210 2156 Fragments arranged by size: 2156 1069 210 172 144 124 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 258 448 2083 2393 2539 3685 258 Size: 190 1635 310 146 1146 448 Fragments arranged by size: 1635 1146 448 310 190 146 EcoICRI GAG'CTC Cuts at: 632 887 632 Size: 255 3620 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 557 609 872 1700 2132 2879 3147 557 Size: 52 263 828 432 747 268 1285 Fragments arranged by size: 1285 828 747 432 268 263 52 EcoNI CCTnn'n_nnAGG Cuts at: 185 185 Size: 3875 EcoO109I rG'GnC_Cy Cuts at: 774 1014 774 Size: 240 3635 EcoRI G'AATT_C Cuts at: 300 879 300 Size: 579 3296 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 237 1894 237 Size: 1657 2218 FspI TGC'GCA Cuts at: 1656 1656 Size: 3875 HaeII r_GCGC'y Cuts at: 1309 1557 2579 3558 1309 Size: 248 1022 979 1626 Fragments arranged by size: 1626 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 418 450 511 543 1717 1747 1749 1779 418 Size: 32 61 32 1174 30 2 30 2514 Fragments arranged by size: 2514 1174 61 32 32 30 30 2 HincII GTy'rAC Cuts at: 914 914 Size: 3875 KasI G'GCGC_C Cuts at: 1305 1553 1305 Size: 248 3627 KpnI G_GTAC'C Cuts at: 895 895 Size: 3875 MluI A'CGCG_T Cuts at: 3308 3308 Size: 3875 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2546 2730 2546 Size: 184 3691 MscI TGG'CCA Cuts at: 1636 1636 Size: 3875 MslI CAynn'nnrTG Cuts at: 1864 1991 1864 Size: 127 3748 MspA1I CmG'CkG Cuts at: 792 986 1300 1660 2673 2918 792 Size: 194 314 360 1013 245 1749 Fragments arranged by size: 1749 1013 360 314 245 194 NaeI GCC'GGC Cuts at: 441 2056 441 Size: 1615 2260 NarI GG'CG_CC Cuts at: 1306 1554 1306 Size: 248 3627 NcoI C'CATG_G Cuts at: 107 107 Size: 3875 NgoMIV G'CCGG_C Cuts at: 439 2054 439 Size: 1615 2260 NspI r_CATG'y Cuts at: 928 1428 2335 928 Size: 500 907 2468 Fragments arranged by size: 2468 907 500 PciI A'CATG_T Cuts at: 2331 2331 Size: 3875 PfoI T'CCnGG_A Cuts at: 625 625 Size: 3875 PmeI GTTT'AAAC Cuts at: 874 874 Size: 3875 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3014 3046 3014 Size: 32 3843 PpuMI rG'GwC_Cy Cuts at: 774 774 Size: 3875 PstI C_TGCA'G Cuts at: 922 1607 922 Size: 685 3190 PvuI CG_AT'CG Cuts at: 104 104 Size: 3875 PvuII CAG'CTG Cuts at: 792 1300 1660 792 Size: 508 360 3007 Fragments arranged by size: 3007 508 360 RsrII CG'GwC_CG Cuts at: 2070 2070 Size: 3875 SacI G_AGCT'C Cuts at: 634 889 634 Size: 255 3620 SalI G'TCGA_C Cuts at: 912 912 Size: 3875 SapI GCTCTTCn'nnn_ Cuts at: 1898 2108 1898 Size: 210 3665 SbfI CC_TGCA'GG Cuts at: 922 922 Size: 3875 ScaI AGT'ACT Cuts at: 3392 3392 Size: 3875 SexAI A'CCwGG_T Cuts at: 503 503 Size: 3875 SfcI C'TryA_G Cuts at: 33 182 918 1603 2596 2787 33 Size: 149 736 685 993 191 1121 Fragments arranged by size: 1121 993 736 685 191 149 SfoI GGC'GCC Cuts at: 1307 1555 1307 Size: 248 3627 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 3875 SmaI CCC'GGG Cuts at: 897 897 Size: 3875 SmlI C'TyrA_G Cuts at: 1030 1945 2437 2699 1030 Size: 915 492 262 2206 Fragments arranged by size: 2206 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 3875 SphI G_CATG'C Cuts at: 928 1428 928 Size: 500 3375 StyI C'CwwG_G Cuts at: 107 1009 1363 107 Size: 902 354 2619 Fragments arranged by size: 2619 902 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1458 1591 2050 2259 1458 Size: 133 459 209 3074 Fragments arranged by size: 3074 459 209 133 TatI w'GTAC_w Cuts at: 3390 3390 Size: 3875 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3392 3424 3392 Size: 32 3843 Tth111I GACn'n_nGTC Cuts at: 1672 1672 Size: 3875 XbaI T'CTAG_A Cuts at: 906 906 Size: 3875 XcmI CCAnnnn_n'nnnnTGG Cuts at: 633 1370 633 Size: 737 3138 XmaI C'CCGG_G Cuts at: 895 895 Size: 3875 XmnI GAAnn'nnTTC Cuts at: 220 220 Size: 3875 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PfoI PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AleI AlfI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BglI BmgBI BmtI Bpu10I BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PasI PflMI PmlI PshAI PsiI PspOMI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI ZraI