(Circular) (Six-base) MAPSORT of: pF1KB8306.seq Check: 5030 from: 1 to: 3539 pF1KB8306 3539 bp With 182 enzymes: SgfI * June 20, 2008 10:28 .. AccI GT'mk_AC Cuts at: 577 577 Size: 3539 Acc65I G'GTAC_C Cuts at: 126 555 126 Size: 429 3110 AclI AA'CG_TT Cuts at: 3379 3379 Size: 3539 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 212 1364 1796 2543 2811 212 Size: 1152 432 747 268 940 Fragments arranged by size: 1152 940 747 432 268 AflIII A'CryG_T Cuts at: 891 1995 2972 891 Size: 1104 977 1458 Fragments arranged by size: 1458 1104 977 AgeI A'CCGG_T Cuts at: 1927 1927 Size: 3539 AlwNI CAG_nnn'CTG Cuts at: 243 844 2411 243 Size: 601 1567 1371 Fragments arranged by size: 1567 1371 601 ApaLI G'TGCA_C Cuts at: 2309 2309 Size: 3539 ApoI r'AATT_y Cuts at: 424 543 2892 3148 424 Size: 119 2349 256 815 Fragments arranged by size: 2349 815 256 119 AseI AT'TA_AT Cuts at: 20 20 Size: 3539 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 3539 AvaI C'yCGr_G Cuts at: 559 559 Size: 3539 BamHI G'GATC_C Cuts at: 564 564 Size: 3539 BanI G'GyrC_C Cuts at: 126 555 969 1217 1252 126 Size: 429 414 248 35 2413 Fragments arranged by size: 2413 429 414 248 35 BanII G_rGCy'C Cuts at: 365 529 553 1583 365 Size: 164 24 1030 2321 Fragments arranged by size: 2321 1030 164 24 BbeI G_GCGC'C Cuts at: 973 1221 973 Size: 248 3291 BbsI GAAGACnn'nnnn_ Cuts at: 200 200 Size: 3539 BciVI GTATCCnnnnn_n' Cuts at: 1437 2198 3539 1437 Size: 761 1341 1437 Fragments arranged by size: 1437 1341 761 BclI T'GATC_A Cuts at: 110 1059 110 Size: 949 2590 BglII A'GATC_T Cuts at: 1054 3091 1054 Size: 2037 1502 BlpI GC'TnA_GC Cuts at: 410 651 410 Size: 241 3298 Bme1580I G_kGCm'C Cuts at: 1164 1257 2313 1164 Size: 93 1056 2390 Fragments arranged by size: 2390 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 462 751 832 1167 3311 462 Size: 289 81 335 2144 690 Fragments arranged by size: 2144 690 335 289 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 1906 1906 Size: 3539 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 511 511 Size: 3539 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 337 364 715 1594 2086 2384 337 Size: 27 351 879 492 298 1492 Fragments arranged by size: 1492 879 492 351 298 27 BsaI GGTCTCn'nnnn_ Cuts at: 3243 3243 Size: 3539 BsaAI yAC'GTr Cuts at: 892 892 Size: 3539 BsaBI GATnn'nnATC Cuts at: 6 1053 3090 6 Size: 1047 2037 455 Fragments arranged by size: 2037 1047 455 BsaHI Gr'CG_yC Cuts at: 170 970 1218 1920 3416 170 Size: 800 248 702 1496 293 Fragments arranged by size: 1496 800 702 293 248 BsaWI w'CCGG_w Cuts at: 728 1249 1927 2201 2348 2759 728 Size: 521 678 274 147 411 1508 Fragments arranged by size: 1508 678 521 411 274 147 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 406 457 406 Size: 51 3488 BseYI C'CCAG_C Cuts at: 964 1722 2299 2795 964 Size: 758 577 496 1708 Fragments arranged by size: 1708 758 577 496 BsiEI CG_ry'CG Cuts at: 104 1081 1127 2335 104 Size: 977 46 1208 1308 Fragments arranged by size: 1308 1208 977 46 BsiHKAI G_wGCw'C Cuts at: 365 553 1331 1521 2313 365 Size: 188 778 190 792 1591 Fragments arranged by size: 1591 792 778 190 188 Bsp1286I G_dGCh'C Cuts at: 365 529 553 1164 1257 1331 1521 1583 2313 Size: 164 24 611 93 74 190 62 730 Cuts at: 2313 365 Size: 1591 Fragments arranged by size: 1591 730 611 190 164 93 74 62 24 BspEI T'CCGG_A Cuts at: 2759 2759 Size: 3539 BspHI T'CATG_A Cuts at: 255 2715 255 Size: 2460 1079 BspMI ACCTGCnnnn'nnnn_ Cuts at: 138 589 803 1105 1486 138 Size: 451 214 302 381 2191 Fragments arranged by size: 2191 451 381 302 214 BsrBI CCG'CTC Cuts at: 50 1831 1885 3369 3534 50 Size: 1781 54 1484 165 55 Fragments arranged by size: 1781 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 365 1451 365 Size: 1086 2453 BsrFI r'CCGG_y Cuts at: 1537 1718 1927 1537 Size: 181 209 3149 Fragments arranged by size: 3149 209 181 BssSI C'ACGA_G Cuts at: 1810 2168 1810 Size: 358 3181 BstAPI GCAn_nnn'nTGC Cuts at: 916 916 Size: 3539 BstBI TT'CG_AA Cuts at: 501 1900 501 Size: 1399 2140 BstXI CCAn_nnnn'nTGG Cuts at: 207 207 Size: 3539 BstYI r'GATC_y Cuts at: 1 564 1031 1054 1389 1635 2647 2733 2745 Size: 563 467 23 335 246 1012 86 12 Cuts at: 2745 3091 1 Size: 346 449 Fragments arranged by size: 1012 563 467 449 346 335 246 86 23 12 BtgI C'CryG_G Cuts at: 272 272 Size: 3539 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 135 1489 1670 1833 135 Size: 1354 181 163 1841 Fragments arranged by size: 1841 1354 181 163 BtsI GCAGTG_nn' Cuts at: 291 782 291 Size: 491 3048 DraI TTT'AAA Cuts at: 538 538 Size: 3539 DrdI GACnn_nn'nnGTC Cuts at: 1245 2103 1245 Size: 858 2681 EaeI y'GGCC_r Cuts at: 1124 1298 1689 1716 3061 1124 Size: 174 391 27 1345 1602 Fragments arranged by size: 1602 1345 391 174 27 EagI C'GGCC_G Cuts at: 1124 1124 Size: 3539 EarI CTCTTCn'nnn_ Cuts at: 383 511 1562 1772 383 Size: 128 1051 210 2150 Fragments arranged by size: 2150 1051 210 128 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 1747 2057 2203 3349 1747 Size: 310 146 1146 1937 Fragments arranged by size: 1937 1146 310 146 EcoICRI GAG'CTC Cuts at: 363 551 363 Size: 188 3351 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 212 511 1364 1796 2543 2811 212 Size: 299 853 432 747 268 940 Fragments arranged by size: 940 853 747 432 299 268 EcoO109I rG'GnC_Cy Cuts at: 678 678 Size: 3539 EcoRI G'AATT_C Cuts at: 424 543 424 Size: 119 3420 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 331 1558 331 Size: 1227 2312 FspI TGC'GCA Cuts at: 1320 1320 Size: 3539 HaeII r_GCGC'y Cuts at: 973 1221 2243 3222 973 Size: 248 1022 979 1290 Fragments arranged by size: 1290 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1381 1411 1413 1443 1381 Size: 30 2 30 3477 Fragments arranged by size: 3477 30 30 2 HincII GTy'rAC Cuts at: 578 578 Size: 3539 KasI G'GCGC_C Cuts at: 969 1217 969 Size: 248 3291 KpnI G_GTAC'C Cuts at: 130 559 130 Size: 429 3110 MluI A'CGCG_T Cuts at: 2972 2972 Size: 3539 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2210 2394 2210 Size: 184 3355 MscI TGG'CCA Cuts at: 1300 1300 Size: 3539 MslI CAynn'nnrTG Cuts at: 277 1528 1655 277 Size: 1251 127 2161 Fragments arranged by size: 2161 1251 127 MspA1I CmG'CkG Cuts at: 650 964 1324 2337 2582 650 Size: 314 360 1013 245 1607 Fragments arranged by size: 1607 1013 360 314 245 NaeI GCC'GGC Cuts at: 1720 1720 Size: 3539 NarI GG'CG_CC Cuts at: 970 1218 970 Size: 248 3291 NcoI C'CATG_G Cuts at: 272 272 Size: 3539 NgoMIV G'CCGG_C Cuts at: 1718 1718 Size: 3539 NspI r_CATG'y Cuts at: 592 1092 1999 592 Size: 500 907 2132 Fragments arranged by size: 2132 907 500 PciI A'CATG_T Cuts at: 1995 1995 Size: 3539 PmeI GTTT'AAAC Cuts at: 538 538 Size: 3539 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2678 2710 2678 Size: 32 3507 PstI C_TGCA'G Cuts at: 586 1271 586 Size: 685 2854 PvuI CG_AT'CG Cuts at: 104 104 Size: 3539 PvuII CAG'CTG Cuts at: 964 1324 964 Size: 360 3179 RsrII CG'GwC_CG Cuts at: 1734 1734 Size: 3539 SacI G_AGCT'C Cuts at: 365 553 365 Size: 188 3351 SalI G'TCGA_C Cuts at: 576 576 Size: 3539 SapI GCTCTTCn'nnn_ Cuts at: 1562 1772 1562 Size: 210 3329 SbfI CC_TGCA'GG Cuts at: 586 586 Size: 3539 ScaI AGT'ACT Cuts at: 3056 3056 Size: 3539 SfcI C'TryA_G Cuts at: 33 235 582 1267 2260 2451 33 Size: 202 347 685 993 191 1121 Fragments arranged by size: 1121 993 685 347 202 191 SfoI GGC'GCC Cuts at: 971 1219 971 Size: 248 3291 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 3539 SmaI CCC'GGG Cuts at: 561 561 Size: 3539 SmlI C'TyrA_G Cuts at: 316 343 694 1609 2101 2363 316 Size: 27 351 915 492 262 1492 Fragments arranged by size: 1492 915 492 351 262 27 SpeI A'CTAG_T Cuts at: 67 67 Size: 3539 SphI G_CATG'C Cuts at: 592 1092 592 Size: 500 3039 StyI C'CwwG_G Cuts at: 272 673 1027 272 Size: 401 354 2784 Fragments arranged by size: 2784 401 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1122 1255 1714 1923 1122 Size: 133 459 209 2738 Fragments arranged by size: 2738 459 209 133 TatI w'GTAC_w Cuts at: 3054 3054 Size: 3539 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3056 3088 3056 Size: 32 3507 Tth111I GACn'n_nGTC Cuts at: 1336 1336 Size: 3539 XbaI T'CTAG_A Cuts at: 570 570 Size: 3539 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1034 1034 Size: 3539 XmaI C'CCGG_G Cuts at: 559 559 Size: 3539 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmeI PpiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AleI AlfI AloI ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI Bpu10I BsaXI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI