Restriction map for pF1KB8302
 (Linear) (Six-base) MAPSORT of: KIBB8302.seq  Check: 2590  from: 1  to: 675



KIBB8302.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:04  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0       8     675 
   Size:          8     667
 
AflIII A'CryG_T

Cuts at:      0     509     546     675 
   Size:        509      37     129

  Fragments arranged by size: 

                 509     129      37
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     199     675 
   Size:        199     476
 
AlwNI CAG_nnn'CTG

Cuts at:      0     192     675 
   Size:        192     483
 
ApoI r'AATT_y

Cuts at:      0     103     166     607     675 
   Size:        103      63     441      68

  Fragments arranged by size: 

                 441     103      68      63
 
BanI G'GyrC_C

Cuts at:      0      20     675 
   Size:         20     655
 
BanII G_rGCy'C

Cuts at:      0      31     675 
   Size:         31     644
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      80     258     675 
   Size:         80     178     417

  Fragments arranged by size: 

                 417     178      80
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     163     270     675 
   Size:        163     107     405

  Fragments arranged by size: 

                 405     163     107
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     260     675 
   Size:        260     415
 
BsaAI yAC'GTr

Cuts at:      0     512     549     675 
   Size:        512      37     126

  Fragments arranged by size: 

                 512     126      37
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     253     544     675 
   Size:        253     291     131

  Fragments arranged by size: 

                 291     253     131
 
BseYI C'CCAG_C

Cuts at:      0      33     220     675 
   Size:         33     187     455

  Fragments arranged by size: 

                 455     187      33
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     503     675 
   Size:        503     172
 
Bsp1286I G_dGCh'C

Cuts at:      0      31     675 
   Size:         31     644
 
BspHI T'CATG_A

Cuts at:      0     476     641     675 
   Size:        476     165      34

  Fragments arranged by size: 

                 476     165      34
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0       8     177     675 
   Size:          8     169     498

  Fragments arranged by size: 

                 498     169       8
 
BsrFI r'CCGG_y

Cuts at:      0     128     675 
   Size:        128     547
 
BstEII G'GTnAC_C

Cuts at:      0     594     675 
   Size:        594      81
 
Bsu36I CC'TnA_GG

Cuts at:      0     134     675 
   Size:        134     541
 
DraIII CAC_nnn'GTG

Cuts at:      0     122     675 
   Size:        122     553
 
EarI CTCTTCn'nnn_

Cuts at:      0     313     675 
   Size:        313     362
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     163     270     675 
   Size:        163     107     405

  Fragments arranged by size: 

                 405     163     107
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     436     675 
   Size:        436     239
 
EcoO109I rG'GnC_Cy

Cuts at:      0     189     534     675 
   Size:        189     345     141

  Fragments arranged by size: 

                 345     189     141
 
EcoRI G'AATT_C

Cuts at:      0     607     675 
   Size:        607      68
 
MspA1I CmG'CkG

Cuts at:      0      33      63     150     224     675 
   Size:         33      30      87      74     451

  Fragments arranged by size: 

                 451      87      74      33      30
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     570     675 
   Size:        570     105
 
PfoI T'CCnGG_A

Cuts at:      0     524     675 
   Size:        524     151
 
PmlI CAC'GTG

Cuts at:      0     549     675 
   Size:        549     126
 
PpuMI rG'GwC_Cy

Cuts at:      0     189     675 
   Size:        189     486
 
PstI C_TGCA'G

Cuts at:      0     155     675 
   Size:        155     520
 
PvuII CAG'CTG

Cuts at:      0      63     150     675 
   Size:         63      87     525

  Fragments arranged by size: 

                 525      87      63
 
SfcI C'TryA_G

Cuts at:      0     151     392     675 
   Size:        151     241     283

  Fragments arranged by size: 

                 283     241     151
 
SmlI C'TyrA_G

Cuts at:      0     275     675 
   Size:        275     400
 
StyI C'CwwG_G

Cuts at:      0     455     538     675 
   Size:        455      83     137

  Fragments arranged by size: 

                 455     137      83
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     330     675 
   Size:        330     345
 
TatI w'GTAC_w

Cuts at:      0     506     675 
   Size:        506     169
 
Tth111I GACn'n_nGTC

Cuts at:      0      72     667     675 
   Size:         72     595       8

  Fragments arranged by size: 

                 595      72       8
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      67     675 
   Size:         67     608
 

 Enzymes that do cut:

     AarI   AflIII     AlfI    AlwNI     ApoI     BanI    BanII     BmrI
     BpmI    BpuEI    BsaAI    BseRI    BseYI     BsgI Bsp1286I    BspHI
    BspMI    BsrFI   BstEII   Bsu36I   DraIII     EarI  Eco57MI    EcoNI
 EcoO109I    EcoRI   MspA1I    PflMI     PfoI     PmlI    PpuMI     PstI
    PvuII     SfcI     SmlI     StyI    TaqII     TatI  Tth111I     XcmI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII     AgeI
     AhdI     AleI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BbeI     BbsI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmtI     BplI   Bpu10I     BsaI    BsaBI    BsaHI    BsaWI    BsaXI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BsrBI    BsrDI
    BsrGI   BssHII    BssSI   BstAPI    BstBI    BstXI    BstYI  BstZ17I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI     EaeI
     EagI     EciI  EcoICRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI     MslI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI     PmeI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI     TstI     XbaI
     XhoI     XmaI     XmnI     ZraI
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