(Circular) (Six-base) MAPSORT of: pF1KB8292.seq Check: 6531 from: 1 to: 4460 pF1KB8292 4460 bp With 182 enzymes: SgfI * June 20, 2008 10:28 .. AccI GT'mk_AC Cuts at: 1498 1498 Size: 4460 Acc65I G'GTAC_C Cuts at: 1476 1476 Size: 4460 AclI AA'CG_TT Cuts at: 4300 4300 Size: 4460 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 2285 2717 3464 3732 2285 Size: 432 747 268 3013 Fragments arranged by size: 3013 747 432 268 AflIII A'CryG_T Cuts at: 383 1199 1812 2916 3893 383 Size: 816 613 1104 977 950 Fragments arranged by size: 1104 977 950 816 613 AgeI A'CCGG_T Cuts at: 2848 2848 Size: 4460 AhdI GACnn_n'nnGTC Cuts at: 672 801 672 Size: 129 4331 AleI CACnn'nnGTG Cuts at: 260 1202 260 Size: 942 3518 AlwNI CAG_nnn'CTG Cuts at: 1434 1765 3332 1434 Size: 331 1567 2562 Fragments arranged by size: 2562 1567 331 ApaLI G'TGCA_C Cuts at: 1035 3230 1035 Size: 2195 2265 ApoI r'AATT_y Cuts at: 208 244 1464 3813 4069 208 Size: 36 1220 2349 256 599 Fragments arranged by size: 2349 1220 599 256 36 AseI AT'TA_AT Cuts at: 20 20 Size: 4460 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4460 AvaI C'yCGr_G Cuts at: 654 1480 654 Size: 826 3634 BamHI G'GATC_C Cuts at: 1485 1485 Size: 4460 BanI G'GyrC_C Cuts at: 1476 1890 2138 2173 1476 Size: 414 248 35 3763 Fragments arranged by size: 3763 414 248 35 BanII G_rGCy'C Cuts at: 1474 2504 1474 Size: 1030 3430 BbeI G_GCGC'C Cuts at: 1894 2142 1894 Size: 248 4212 BbsI GAAGACnn'nnnn_ Cuts at: 964 1257 964 Size: 293 4167 BciVI GTATCCnnnnn_n' Cuts at: 466 2358 3119 4460 466 Size: 1892 761 1341 466 Fragments arranged by size: 1892 1341 761 466 BclI T'GATC_A Cuts at: 156 1980 156 Size: 1824 2636 BglI GCCn_nnn'nGGC Cuts at: 946 946 Size: 4460 BglII A'GATC_T Cuts at: 1975 4012 1975 Size: 2037 2423 BlpI GC'TnA_GC Cuts at: 1572 1572 Size: 4460 Bme1580I G_kGCm'C Cuts at: 1039 1143 2085 2178 3234 1039 Size: 104 942 93 1056 2265 Fragments arranged by size: 2265 1056 942 104 93 BmrI ACTGGGnnnn_n' Cuts at: 1361 1672 1753 2088 4232 1361 Size: 311 81 335 2144 1589 Fragments arranged by size: 2144 1589 335 311 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2827 2827 Size: 4460 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1456 1456 Size: 4460 Bpu10I CC'TnA_GC Cuts at: 691 691 Size: 4460 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 331 800 1219 1636 2515 3007 3305 331 Size: 469 419 417 879 492 298 1486 Fragments arranged by size: 1486 879 492 469 419 417 298 BsaI GGTCTCn'nnnn_ Cuts at: 772 4164 772 Size: 3392 1068 BsaAI yAC'GTr Cuts at: 1813 1813 Size: 4460 BsaBI GATnn'nnATC Cuts at: 6 1974 4011 6 Size: 1968 2037 455 Fragments arranged by size: 2037 1968 455 BsaHI Gr'CG_yC Cuts at: 1891 2139 2841 4337 1891 Size: 248 702 1496 2014 Fragments arranged by size: 2014 1496 702 248 BsaWI w'CCGG_w Cuts at: 1649 2170 2848 3122 3269 3680 1649 Size: 521 678 274 147 411 2429 Fragments arranged by size: 2429 678 521 411 274 147 BseYI C'CCAG_C Cuts at: 720 1885 2643 3220 3716 720 Size: 1165 758 577 496 1464 Fragments arranged by size: 1464 1165 758 577 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1272 1272 Size: 4460 BsiEI CG_ry'CG Cuts at: 104 2002 2048 3256 104 Size: 1898 46 1208 1308 Fragments arranged by size: 1898 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 255 1039 1474 2252 2442 3234 255 Size: 784 435 778 190 792 1481 Fragments arranged by size: 1481 792 784 778 435 190 BsmBI CGTCTCn'nnnn_ Cuts at: 1432 1432 Size: 4460 Bsp1286I G_dGCh'C Cuts at: 255 1039 1143 1474 2085 2178 2252 2442 2504 Size: 784 104 331 611 93 74 190 62 Cuts at: 2504 3234 255 Size: 730 1481 Fragments arranged by size: 1481 784 730 611 331 190 104 93 74 62 BspEI T'CCGG_A Cuts at: 3680 3680 Size: 4460 BspHI T'CATG_A Cuts at: 1242 3636 1242 Size: 2394 2066 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1347 1510 1724 2026 2407 1347 Size: 163 214 302 381 3400 Fragments arranged by size: 3400 381 302 214 163 BsrBI CCG'CTC Cuts at: 50 2752 2806 4290 4455 50 Size: 2702 54 1484 165 55 Fragments arranged by size: 2702 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 2372 2372 Size: 4460 BsrFI r'CCGG_y Cuts at: 2458 2639 2848 2458 Size: 181 209 4070 Fragments arranged by size: 4070 209 181 BsrGI T'GTAC_A Cuts at: 386 386 Size: 4460 BssSI C'ACGA_G Cuts at: 1327 2731 3089 1327 Size: 1404 358 2698 Fragments arranged by size: 2698 1404 358 BstAPI GCAn_nnn'nTGC Cuts at: 1837 1837 Size: 4460 BstBI TT'CG_AA Cuts at: 2821 2821 Size: 4460 BstEII G'GTnAC_C Cuts at: 919 1359 919 Size: 440 4020 BstXI CCAn_nnnn'nTGG Cuts at: 952 952 Size: 4460 BstYI r'GATC_y Cuts at: 1 1485 1952 1975 2310 2556 3568 3654 3666 Size: 1484 467 23 335 246 1012 86 12 Cuts at: 3666 4012 1 Size: 346 449 Fragments arranged by size: 1484 1012 467 449 346 335 246 86 23 12 BtgI C'CryG_G Cuts at: 107 773 107 Size: 666 3794 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1423 2410 2591 2754 1423 Size: 987 181 163 3129 Fragments arranged by size: 3129 987 181 163 BtsI GCAGTG_nn' Cuts at: 1703 1703 Size: 4460 DraI TTT'AAA Cuts at: 291 1459 291 Size: 1168 3292 DrdI GACnn_nn'nnGTC Cuts at: 2166 3024 2166 Size: 858 3602 EaeI y'GGCC_r Cuts at: 756 2045 2219 2610 2637 3982 756 Size: 1289 174 391 27 1345 1234 Fragments arranged by size: 1345 1289 1234 391 174 27 EagI C'GGCC_G Cuts at: 2045 2045 Size: 4460 EarI CTCTTCn'nnn_ Cuts at: 362 2483 2693 362 Size: 2121 210 2129 Fragments arranged by size: 2129 2121 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 1024 2668 2978 3124 4270 1024 Size: 1644 310 146 1146 1214 Fragments arranged by size: 1644 1214 1146 310 146 EcoICRI GAG'CTC Cuts at: 1472 1472 Size: 4460 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1456 2285 2717 3464 3732 1456 Size: 829 432 747 268 2184 Fragments arranged by size: 2184 829 747 432 268 EcoO109I rG'GnC_Cy Cuts at: 372 803 1599 372 Size: 431 796 3233 Fragments arranged by size: 3233 796 431 EcoRI G'AATT_C Cuts at: 1464 1464 Size: 4460 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2479 2479 Size: 4460 FspI TGC'GCA Cuts at: 2241 2241 Size: 4460 HaeII r_GCGC'y Cuts at: 1894 2142 3164 4143 1894 Size: 248 1022 979 2211 Fragments arranged by size: 2211 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 658 690 787 819 2302 2332 2334 2364 658 Size: 32 97 32 1483 30 2 30 2754 Fragments arranged by size: 2754 1483 97 32 32 30 30 2 HincII GTy'rAC Cuts at: 423 1499 423 Size: 1076 3384 HindIII A'AGCT_T Cuts at: 295 307 295 Size: 12 4448 HpaI GTT'AAC Cuts at: 423 423 Size: 4460 KasI G'GCGC_C Cuts at: 1890 2138 1890 Size: 248 4212 KpnI G_GTAC'C Cuts at: 1480 1480 Size: 4460 MfeI C'AATT_G Cuts at: 747 747 Size: 4460 MluI A'CGCG_T Cuts at: 3893 3893 Size: 4460 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 3131 3315 3131 Size: 184 4276 MscI TGG'CCA Cuts at: 2221 2221 Size: 4460 MslI CAynn'nnrTG Cuts at: 124 258 260 1175 1202 2449 2576 124 Size: 134 2 915 27 1247 127 2008 Fragments arranged by size: 2008 1247 915 134 127 27 2 MspA1I CmG'CkG Cuts at: 1434 1571 1885 2245 3258 3503 1434 Size: 137 314 360 1013 245 2391 Fragments arranged by size: 2391 1013 360 314 245 137 NaeI GCC'GGC Cuts at: 2641 2641 Size: 4460 NarI GG'CG_CC Cuts at: 1891 2139 1891 Size: 248 4212 NcoI C'CATG_G Cuts at: 107 773 107 Size: 666 3794 NgoMIV G'CCGG_C Cuts at: 2639 2639 Size: 4460 NspI r_CATG'y Cuts at: 387 1203 1513 2013 2920 387 Size: 816 310 500 907 1927 Fragments arranged by size: 1927 907 816 500 310 PciI A'CATG_T Cuts at: 383 1199 2916 383 Size: 816 1717 1927 Fragments arranged by size: 1927 1717 816 PmeI GTTT'AAAC Cuts at: 1459 1459 Size: 4460 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3599 3631 3599 Size: 32 4428 PpuMI rG'GwC_Cy Cuts at: 372 803 372 Size: 431 4029 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 370 402 370 Size: 32 4428 PstI C_TGCA'G Cuts at: 280 1507 2192 280 Size: 1227 685 2548 Fragments arranged by size: 2548 1227 685 PvuI CG_AT'CG Cuts at: 104 104 Size: 4460 PvuII CAG'CTG Cuts at: 1434 1885 2245 1434 Size: 451 360 3649 Fragments arranged by size: 3649 451 360 RsrII CG'GwC_CG Cuts at: 2655 2655 Size: 4460 SacI G_AGCT'C Cuts at: 1474 1474 Size: 4460 SalI G'TCGA_C Cuts at: 1497 1497 Size: 4460 SanDI GG'GwC_CC Cuts at: 803 803 Size: 4460 SapI GCTCTTCn'nnn_ Cuts at: 2483 2693 2483 Size: 210 4250 SbfI CC_TGCA'GG Cuts at: 1507 1507 Size: 4460 ScaI AGT'ACT Cuts at: 3977 3977 Size: 4460 SfcI C'TryA_G Cuts at: 33 276 1104 1449 1503 2188 3181 3372 33 Size: 243 828 345 54 685 993 191 1121 Fragments arranged by size: 1121 993 828 685 345 243 191 54 SfoI GGC'GCC Cuts at: 1892 2140 1892 Size: 248 4212 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4460 SmaI CCC'GGG Cuts at: 1482 1482 Size: 4460 SmlI C'TyrA_G Cuts at: 310 815 1234 1615 2530 3022 3284 310 Size: 505 419 381 915 492 262 1486 Fragments arranged by size: 1486 915 505 492 419 381 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4460 SphI G_CATG'C Cuts at: 1513 2013 1513 Size: 500 3960 StyI C'CwwG_G Cuts at: 107 773 1292 1304 1594 1948 107 Size: 666 519 12 290 354 2619 Fragments arranged by size: 2619 666 519 354 290 12 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 318 595 2043 2176 2635 2844 318 Size: 277 1448 133 459 209 1934 Fragments arranged by size: 1934 1448 459 277 209 133 TatI w'GTAC_w Cuts at: 386 3975 386 Size: 3589 871 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 460 492 3977 4009 460 Size: 32 3485 32 911 Fragments arranged by size: 3485 911 32 32 Tth111I GACn'n_nGTC Cuts at: 2257 2257 Size: 4460 XbaI T'CTAG_A Cuts at: 1491 1491 Size: 4460 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1955 1955 Size: 4460 XmaI C'CCGG_G Cuts at: 1480 1480 Size: 4460 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmeI PpiI PpuMI PsrI PstI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AlfI AloI ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI BsaXI BseRI BsiWI BsmI BssHII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI NdeI NheI NotI NruI NsiI PacI PasI PflMI PfoI PmlI PshAI PsiI PspOMI PspXI SacII SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI