Restriction map for pF1KB8291
##################################
# NO CUT ENZYMES (  128 enzymes) #
##################################

AatII       Acc65I      AccII       AclI        AcyI        
AfaI        AflII       AflIII      AhdI        Alw44I      
AocI        ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspI        AvaI        
AviII       AvrII       BamHI       BbeI        BbrPI       
BbsI        BcgI        BciVI       BclI        BfrI        
BglII       BlnI        BlpI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaHI       BsaI        BsiEI       
BsiWI       BsmBI       BsmI        BsoBI       Bsp1407I    
BspDI       BspHI       BspLU11I    BsrDI       BsrGI       
BssHII      BssSI       BstBI       BstUI       Bsu36I      
CelII       ClaI        CpoI        DraI        DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco52I      
Eco81I      EcoNI       EcoRV       EcoT22I     FbaI        
FseI        FspI        Hin1I       HindIII     KasI        
KpnI        KspI        MaeII       MamI        MfeI        
MluI        MunI        MvnI        NaeI        NarI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PmaCI       PmeI        PmlI        PshBI       Psp1406I    
PvuI        RcaI        RsaI        RsrII       SacI        
SacII       SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        Sse8387I    SspBI       SspI        
StuI        SwaI        Tth111I     XbaI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   34 enzymes) #
###################################

AccIII        619  TCCGGA               T^CCGGA
AcsI          600  GAATTC               [GA]^AATT[CT]
AgeI          735  ACCGGT               A^CCGGT
Aor51HI       663  AGCGCT               AGC^GCT
ApaI          544  GGGCCC               GGGCC^C
ApoI          600  GAATTC               [GA]^AATT[CT]
AspHI         355  GAGCAC               G[AT]GC[AT]^C
BcnI         1025  CCCGG                CC^[GC]GG
BfaI          538  CTAG                 C^TAG
BglI         1237  GCCACCATGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BseAI         619  TCCGGA               T^CCGGA
BsgI         1268  CTGCAC               GTGCAG(16/14)
BsiHKAI       355  GAGCAC               G[AT]GC[AT]^C
BspEI         619  TCCGGA               T^CCGGA
Bst1107I      220  GTATAC               GTA^TAC
BstXI        1196  CCATCAACTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I       220  GTATAC               GTA^TAC
Eco47III      663  AGCGCT               AGC^GCT
EcoRI         600  GAATTC               G^AATTC
HaeII         663  AGCGCT               [GA]GCGC^[CT]
HgaI          249  GACGC                GACGC(5/10)
HpaI          817  GTTAAC               GTT^AAC
MaeI          538  CTAG                 C^TAG
MroI          619  TCCGGA               T^CCGGA
MseI          818  TTAA                 T^TAA
NciI         1025  CCCGG                CC^[GC]GG
PflMI         429  CCACCTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI         735  ACCGGT               A^CCGGT
SalI         1354  GTCGAC               G^TCGAC
SapI          918  GCTCTTC              GCTCTTC(1/4)
SphI          891  GCATGC               GCATG^C
Tru9I         818  TTAA                 T^TAA
Van91I        429  CCACCTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          308  CCAGCTTTGATGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AccI          220  GTATAC               GT^[AC][GT]AC
             1354  GTCGAC              
AlwI          801  GATCC                GGATC(4/5)
             1069  GATCC               
BalI           41  TGGCCA               TGG^CCA
             1244  TGGCCA              
BanI          227  GGCACC               G^G[CT][GA]CC
             1168  GGTGCC              
BanII         285  GGGCTC               G[GA]GC[CT]^C
              544  GGGCCC              
BbvCI         143  CCTCAGC              CCTCAGC(-5/-2)
              659  CCTCAGC             
BsaWI         619  TCCGGA               [AT]^CCGG[AT]
              735  ACCGGT              
BsmAI         625  GTCTC                GTCTC(1/5)
             1098  GTCTC               
BsrBI          26  CCGCTC               CCGCTC(-3/-3)
              241  CCGCTC              
BsrFI         735  ACCGGT               [GA]^CCGG[CT]
             1106  ACCGGC              
BstAPI        927  GCATCTCATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1041  GCAGCCCATGC         
BstYI         800  AGATCC               [GA]^GATC[CT]
             1068  AGATCC              
CfoI          664  GCGC                 GCG^C
             1032  GCGC                
Cfr10I        735  ACCGGT               [GA]^CCGG[CT]
             1106  ACCGGC              
EaeI           41  TGGCCA               [CT]^GGCC[GA]
             1244  TGGCCA              
HhaI          664  GCGC                 GCG^C
             1032  GCGC                
HinP1I        664  GCGC                 G^CGC
             1032  GCGC                
MflI          800  AGATCC               [GA]^GATC[CT]
             1068  AGATCC              
MluNI          41  TGGCCA               TGG^CCA
             1244  TGGCCA              
MscI           41  TGGCCA               TGG^CCA
             1244  TGGCCA              
MspA1I        146  CAGCTG               C[AC]G^C[GT]G
              196  CAGCTG              
NcoI          743  CCATGG               C^CATGG
             1241  CCATGG              
NspI            9  ACATGC               [GA]CATG^[CT]
              891  GCATGC              
PpuMI         416  AGGTCCT              [GA]G^G[AT]CC[CT]
              587  AGGACCT             
PshAI         690  GACAACCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             1371  GACAAATGTC          
PvuII         146  CAGCTG               CAG^CTG
              196  CAGCTG              
TfiI          425  GAATC                G^A[AT]TC
             1121  GATTC               
Tsp45I        769  GTCAC                ^GT[GC]AC
             1403  GTGAC               
Tsp509I       601  AATT                 ^AATT
              610  AATT                
XhoII         800  AGATCC               [GA]^GATC[CT]
             1068  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   66 enzymes) #
#########################################

AciI        AluI        AlwNI       AvaII       BbvI        
BmyI        BpmI        BsaJI       BseRI       BsiYI       
BslI        BsmFI       Bsp1286I    BspMI       BsrI        
BssKI       BstEII      BstF5I      BstNI       BstPI       
Cac8I       Cfr13I      DdeI        DpnII       DraII       
DsaI        EarI        EcoO109I    EcoO65I     EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeIII      
HapII       HincII      HindII      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeIII      MboI        
MboII       MnlI        MslI        MspI        MvaI        
MwoI        NdeII       NlaIII      NlaIV       PleI        
PstI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        StyI        TaqI        TseI        TspRI       
TthHB8I     
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