Restriction map for pF1KB8282
##################################
# NO CUT ENZYMES (  152 enzymes) #
##################################

Acc65I      AccI        AccIII      AclI        AcsI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AlwNI       AocI        Aor51HI     ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AvaII       AviII       AvrII       BalI        
BamHI       BbeI        BbrPI       BbvCI       BcgI        
BciVI       BclI        BfaI        BfrI        BglI        
BglII       BlnI        BlpI        BpmI        Bpu1102I    
BsaAI       BsaBI       BsaWI       BseAI       BsgI        
BsiEI       BsiWI       Bsp1407I    BspDI       BspEI       
BspLU11I    BspMI       BsrDI       BsrFI       BsrGI       
BsrI        Bst1107I    BstBI       BstEII      BstPI       
BstXI       BstYI       BstZ17I     Bsu36I      CelII       
Cfr10I      ClaI        CpoI        DraI        DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO65I     EcoRI       
EcoRV       EcoT14I     EcoT22I     FauI        FbaI        
FseI        FspI        HaeII       HgaI        HindIII     
HpaI        KasI        KpnI        KspI        MaeI        
MamI        MflI        MluI        MluNI       MroI        
MscI        MslI        NaeI        NarI        NcoI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflMI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshBI       Psp1406I    PvuI        RsrII       SacI        
SacII       SalI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        StyI        SwaI        Van91I      
XbaI        XcmI        XhoI        XhoII       XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AatII          28  GACGTC               GACGT^C
AccII         424  CGCG                 CG^CG
AcyI           28  GACGTC               G[GA]^CG[CT]C
ApaI          829  GGGCCC               GGGCC^C
AspHI         526  GTGCTC               G[AT]GC[AT]^C
AspI          109  GACCGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          418  CCCGAG               C^[CT]CG[GA]G
BanI          715  GGCACC               G^G[CT][GA]CC
BanII         829  GGGCCC               G[GA]GC[CT]^C
BsaHI          28  GACGTC               G[GA]^CG[CT]C
BsaI          891  GAGACC               GGTCTC(1/5)
BsiHKAI       526  GTGCTC               G[AT]GC[AT]^C
BsmBI         745  GAGACG               CGTCTC(1/5)
BsmFI          26  GGGAC                GGGAC(10/14)
BsmI          619  GAATGC               GAATGC(1/-1)
BsoBI         418  CCCGAG               C^[CT]CG[GA]G
BspHI         155  TCATGA               T^CATGA
BsrBI         787  GAGCGG               CCGCTC(-3/-3)
BssHII        423  GCGCGC               G^CGCGC
BssSI         888  CACGAG               CACGAG(-5/-1)
BstAPI        437  GCAGTATTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI         424  CGCG                 CG^CG
DsaI          481  CCGTGG               C^C[GA][CT]GG
EaeI          266  TGGCCG               [CT]^GGCC[GA]
Hin1I          28  GACGTC               G[GA]^CG[CT]C
HincII        241  GTCAAC               GT[CT]^[GA]AC
HindII        241  GTCAAC               GT[CT]^[GA]AC
MfeI          492  CAATTG               C^AATTG
MspA1I        364  CAGCTG               C[AC]G^C[GT]G
MunI          492  CAATTG               C^AATTG
MvnI          424  CGCG                 CG^CG
NdeI          732  CATATG               CA^TATG
PflFI         109  GACCGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PleI           70  GACTC                GAGTC(4/5)
PshAI         195  GACTTTCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII         364  CAGCTG               CAG^CTG
RcaI          155  TCATGA               T^CATGA
SapI          528  GCTCTTC              GCTCTTC(1/4)
SfaNI          36  GCATC                GCATC(5/9)
SmlI          673  CTTGAG               C^T[CT][GA]AG
Tsp509I       493  AATT                 ^AATT
Tth111I       109  GACCGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AfaI          231  GTAC                 GT^AC
              322  GTAC                
AlwI          570  GATCC                GGATC(4/5)
              468  GGATC               
BbsI          552  GTCTTC               GAAGAC(2/6)
              680  GTCTTC              
BpuAI         552  GTCTTC               GAAGAC(2/6)
              680  GTCTTC              
BsmAI         745  GAGAC                GTCTC(1/5)
              891  GAGAC               
CfoI          423  GCGC                 GCG^C
              425  GCGC                
DdeI          292  CTGAG                C^T[ACGT]AG
              918  CTCAG               
DraII         828  GGGGCCC              [GA]G^G[ACGT]CC[CT]
              829  GGGCCCC             
EarI           72  CTCTTC               CTCTTC(1/4)
              529  CTCTTC              
EcoO109I      828  GGGGCCC              [GA]G^G[ACGT]CC[CT]
              829  GGGCCCC             
HhaI          423  GCGC                 GCG^C
              425  GCGC                
HinP1I        423  GCGC                 G^CGC
              425  GCGC                
Ksp632I        72  CTCTTC               CTCTTC(1/4)
              529  CTCTTC              
MaeIII        128  GTGAC                ^GT[ACGT]AC
              193  GTGAC               
MseI          257  TTAA                 T^TAA
              413  TTAA                
PstI          435  CTGCAG               CTGCA^G
              701  CTGCAG              
RsaI          231  GTAC                 GT^AC
              322  GTAC                
SfcI          435  CTGCAG               C^T[GA][CT]AG
              701  CTGCAG              
TaqI           32  TCGA                 T^CGA
              755  TCGA                
TfiI          510  GAATC                G^A[AT]TC
              852  GAATC               
Tru9I         257  TTAA                 T^TAA
              413  TTAA                
Tsp45I        128  GTGAC                ^GT[GC]AC
              193  GTGAC               
TthHB8I        32  TCGA                 T^CGA
              755  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   41 enzymes) #
#########################################

AciI        AluI        BbvI        BcnI        BmyI        
BsaJI       BseRI       BsiYI       BslI        Bsp1286I    
BssKI       BstF5I      BstNI       Cac8I       Cfr13I      
DpnII       EcoRII      Fnu4HI      FokI        HaeIII      
HapII       HinfI       HpaII       HphI        ItaI        
MaeII       MboI        MboII       MnlI        MspI        
MvaI        MwoI        NciI        NdeII       NlaIII      
NlaIV       Sau3AI      Sau96I      ScrFI       TseI        
TspRI       
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