Restriction map for pF1KB8282
 (Linear) (Six-base) MAPSORT of: KIBB8282.seq  Check: 5082  from: 1  to: 924



KIBB8282.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:04  ..

AatII G_ACGT'C

Cuts at:      0      32     924 
   Size:         32     892
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     388     539     924 
   Size:        388     151     385

  Fragments arranged by size: 

                 388     385     151
 
ApaI G_GGCC'C

Cuts at:      0     833     924 
   Size:        833      91
 
AvaI C'yCGr_G

Cuts at:      0     418     924 
   Size:        418     506
 
BanI G'GyrC_C

Cuts at:      0     715     924 
   Size:        715     209
 
BanII G_rGCy'C

Cuts at:      0     833     924 
   Size:        833      91
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     545     673     924 
   Size:        545     128     251

  Fragments arranged by size: 

                 545     251     128
 
Bme1580I G_kGCm'C

Cuts at:      0     397     833     924 
   Size:        397     436      91

  Fragments arranged by size: 

                 436     397      91
 
BmgBI CAC'GTC

Cuts at:      0     102     161     924 
   Size:        102      59     763

  Fragments arranged by size: 

                 763     102      59
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     694     924 
   Size:        694     230
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     885     924 
   Size:        885      39
 
BsaHI Gr'CG_yC

Cuts at:      0      29     924 
   Size:         29     895
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0       5      35     924 
   Size:          5      30     889

  Fragments arranged by size: 

                 889      30       5
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      75      78     832     924 
   Size:         75       3     754      92

  Fragments arranged by size: 

                 754      92      75       3
 
BsiHKAI G_wGCw'C

Cuts at:      0     530     924 
   Size:        530     394
 
BsmI GAATG_Cn'

Cuts at:      0     625     924 
   Size:        625     299
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     739     924 
   Size:        739     185
 
Bsp1286I G_dGCh'C

Cuts at:      0     397     530     833     924 
   Size:        397     133     303      91

  Fragments arranged by size: 

                 397     303     133      91
 
BspHI T'CATG_A

Cuts at:      0     155     924 
   Size:        155     769
 
BsrBI CCG'CTC

Cuts at:      0     789     924 
   Size:        789     135
 
BssHII G'CGCG_C

Cuts at:      0     423     924 
   Size:        423     501
 
BssSI C'ACGA_G

Cuts at:      0     888     924 
   Size:        888      36
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     443     924 
   Size:        443     481
 
BtgI C'CryG_G

Cuts at:      0     481     924 
   Size:        481     443
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     813     924 
   Size:        813     111
 
BtsI GCAGTG_nn'

Cuts at:      0     432     474     616     630     698     924 
   Size:        432      42     142      14      68     226

  Fragments arranged by size: 

                 432     226     142      68      42      14
 
EaeI y'GGCC_r

Cuts at:      0     266     924 
   Size:        266     658
 
EarI CTCTTCn'nnn_

Cuts at:      0      78     535     924 
   Size:         78     457     389

  Fragments arranged by size: 

                 457     389      78
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     388     539     924 
   Size:        388     151     385

  Fragments arranged by size: 

                 388     385     151
 
EcoO109I rG'GnC_Cy

Cuts at:      0     829     830     924 
   Size:        829       1      94

  Fragments arranged by size: 

                 829      94       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     837     924 
   Size:        837      87
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0       5      37      61      93     924 
   Size:          5      32      24      32     831

  Fragments arranged by size: 

                 831      32      32      24       5
 
HincII GTy'rAC

Cuts at:      0     243     924 
   Size:        243     681
 
MfeI C'AATT_G

Cuts at:      0     492     924 
   Size:        492     432
 
MspA1I CmG'CkG

Cuts at:      0     366     924 
   Size:        366     558
 
NdeI CA'TA_TG

Cuts at:      0     733     924 
   Size:        733     191
 
PasI CC'CwG_GG

Cuts at:      0     313     924 
   Size:        313     611
 
PfoI T'CCnGG_A

Cuts at:      0      23     924 
   Size:         23     901
 
PshAI GACnn'nnGTC

Cuts at:      0     199     924 
   Size:        199     725
 
PspOMI G'GGCC_C

Cuts at:      0     829     924 
   Size:        829      95
 
PstI C_TGCA'G

Cuts at:      0     439     705     924 
   Size:        439     266     219

  Fragments arranged by size: 

                 439     266     219
 
PvuII CAG'CTG

Cuts at:      0     366     924 
   Size:        366     558
 
SapI GCTCTTCn'nnn_

Cuts at:      0     535     924 
   Size:        535     389
 
SfcI C'TryA_G

Cuts at:      0     435     701     924 
   Size:        435     266     223

  Fragments arranged by size: 

                 435     266     223
 
SmlI C'TyrA_G

Cuts at:      0     673     924 
   Size:        673     251
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     474     733     924 
   Size:        474     259     191

  Fragments arranged by size: 

                 474     259     191
 
Tth111I GACn'n_nGTC

Cuts at:      0     112     924 
   Size:        112     812
 
ZraI GAC'GTC

Cuts at:      0      30     924 
   Size:         30     894
 

 Enzymes that do cut:

    AatII     AcuI     ApaI     AvaI     BanI    BanII     BbsI Bme1580I
    BmgBI    BpuEI     BsaI    BsaHI    BsaXI    BseRI  BsiHKAI     BsmI
    BsmBI Bsp1286I    BspHI    BsrBI   BssHII    BssSI   BstAPI     BtgI
    BtgZI     BtsI     EaeI     EarI  Eco57MI EcoO109I     FalI    Hin4I
   HincII     MfeI   MspA1I     NdeI     PasI     PfoI    PshAI   PspOMI
     PstI    PvuII     SapI     SfcI     SmlI    TaqII  Tth111I     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI     BmrI     BmtI
     BplI     BpmI   Bpu10I    BsaAI    BsaBI    BsaWI    BseYI     BsgI
    BsiEI    BsiWI    BspEI    BspMI    BsrDI    BsrFI    BsrGI    BstBI
   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     ClaI    CspCI     DraI
   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV
     FseI     FspI    FspAI    HaeII  HindIII     HpaI     KasI     KpnI
     MluI     MmeI     MscI     MslI     NaeI     NarI     NcoI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PciI    PflMI
     PmeI     PmlI     PpiI    PpuMI     PsiI    PspXI     PsrI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     StyI     SwaI     TatI     TstI     XbaI
     XcmI     XhoI     XmaI     XmnI
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