Restriction map for pF1KB8274
 (Circular) (Six-base) MAPSORT of: pF1KB8274.seq  Check: 4297  from: 1  to: 3419



pF1KB8274  3419 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:28  ..

AccI GT'mk_AC

Cuts at:    457     457 
   Size:       3419
 
Acc65I G'GTAC_C

Cuts at:    435     435 
   Size:       3419
 
AclI AA'CG_TT

Cuts at:   3259    3259 
   Size:       3419
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    178     364    1244    1676    2423    2691     178 
   Size:        186     880     432     747     268     906

  Fragments arranged by size: 

                 906     880     747     432     268     186
 
AflIII A'CryG_T

Cuts at:    771    1875    2852     771 
   Size:       1104     977    1338

  Fragments arranged by size: 

                1338    1104     977
 
AgeI A'CCGG_T

Cuts at:   1807    1807 
   Size:       3419
 
AlwNI CAG_nnn'CTG

Cuts at:    724    2291     724 
   Size:       1567    1852
 
ApaI G_GGCC'C

Cuts at:    338     338 
   Size:       3419
 
ApaLI G'TGCA_C

Cuts at:   2189    2189 
   Size:       3419
 
ApoI r'AATT_y

Cuts at:    423    2772    3028     423 
   Size:       2349     256     814

  Fragments arranged by size: 

                2349     814     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3419
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3419
 
AvaI C'yCGr_G

Cuts at:    439     439 
   Size:       3419
 
BamHI G'GATC_C

Cuts at:    375     444     375 
   Size:         69    3350
 
BanI G'GyrC_C

Cuts at:    435     849    1097    1132     435 
   Size:        414     248      35    2722

  Fragments arranged by size: 

                2722     414     248      35
 
BanII G_rGCy'C

Cuts at:    338     433    1463     338 
   Size:         95    1030    2294

  Fragments arranged by size: 

                2294    1030      95
 
BbeI G_GCGC'C

Cuts at:    853    1101     853 
   Size:        248    3171
 
BbsI GAAGACnn'nnnn_

Cuts at:    397     397 
   Size:       3419
 
BbvCI CC'TCA_GC

Cuts at:    242     242 
   Size:       3419
 
BciVI GTATCCnnnnn_n'

Cuts at:   1317    2078    3419    1317 
   Size:        761    1341    1317

  Fragments arranged by size: 

                1341    1317     761
 
BclI T'GATC_A

Cuts at:    939     939 
   Size:       3419
 
BglII A'GATC_T

Cuts at:    934    2971     934 
   Size:       2037    1382
 
BlpI GC'TnA_GC

Cuts at:    531     531 
   Size:       3419
 
Bme1580I G_kGCm'C

Cuts at:    338    1044    1137    2193     338 
   Size:        706      93    1056    1564

  Fragments arranged by size: 

                1564    1056     706      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    631     712    1047    3191     631 
   Size:         81     335    2144     859

  Fragments arranged by size: 

                2144     859     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1786    1786 
   Size:       3419
 
Bpu10I CC'TnA_GC

Cuts at:    242     242 
   Size:       3419
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    595    1474    1966    2264     595 
   Size:        879     492     298    1750

  Fragments arranged by size: 

                1750     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3123    3123 
   Size:       3419
 
BsaAI yAC'GTr

Cuts at:    772     772 
   Size:       3419
 
BsaBI GATnn'nnATC

Cuts at:      6     933    2970       6 
   Size:        927    2037     455

  Fragments arranged by size: 

                2037     927     455
 
BsaHI Gr'CG_yC

Cuts at:    850    1098    1800    3296     850 
   Size:        248     702    1496     973

  Fragments arranged by size: 

                1496     973     702     248
 
BsaWI w'CCGG_w

Cuts at:    608    1129    1807    2081    2228    2639     608 
   Size:        521     678     274     147     411    1388

  Fragments arranged by size: 

                1388     678     521     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    196     238     241     244     259     292     376     196 
   Size:         42       3       3      15      33      84    3239

  Fragments arranged by size: 

                3239      84      42      33      15       3       3
 
BseYI C'CCAG_C

Cuts at:    844    1602    2179    2675     844 
   Size:        758     577     496    1588

  Fragments arranged by size: 

                1588     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104     137     961    1007    2215     104 
   Size:         33     824      46    1208    1308

  Fragments arranged by size: 

                1308    1208     824      46      33
 
BsiHKAI G_wGCw'C

Cuts at:    433    1211    1401    2193     433 
   Size:        778     190     792    1659

  Fragments arranged by size: 

                1659     792     778     190
 
Bsp1286I G_dGCh'C

Cuts at:    338     433    1044    1137    1211    1401    1463    2193     338 
   Size:         95     611      93      74     190      62     730    1564

  Fragments arranged by size: 

                1564     730     611     190      95      93      74      62
            
 
BspEI T'CCGG_A

Cuts at:   2639    2639 
   Size:       3419
 
BspHI T'CATG_A

Cuts at:   2595    2595 
   Size:       3419
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    469     683     985    1366     469 
   Size:        214     302     381    2522

  Fragments arranged by size: 

                2522     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    1711    1765    3249    3414      50 
   Size:       1661      54    1484     165      55

  Fragments arranged by size: 

                1661    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1331    1331 
   Size:       3419
 
BsrFI r'CCGG_y

Cuts at:   1417    1598    1807    1417 
   Size:        181     209    3029

  Fragments arranged by size: 

                3029     209     181
 
BssSI C'ACGA_G

Cuts at:   1690    2048    1690 
   Size:        358    3061
 
BstAPI GCAn_nnn'nTGC

Cuts at:    796     796 
   Size:       3419
 
BstBI TT'CG_AA

Cuts at:   1780    1780 
   Size:       3419
 
BstYI r'GATC_y

Cuts at:      1     375     444     911     934    1269    1515    2527    2613 
   Size:        374      69     467      23     335     246    1012      86

Cuts at:   2613    2625    2971       1 
   Size:         12     346     449

  Fragments arranged by size: 

                1012     467     449     374     346     335     246      86
                  69      23      12
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1369    1550    1713    1369 
   Size:        181     163    3075

  Fragments arranged by size: 

                3075     181     163
 
BtsI GCAGTG_nn'

Cuts at:    662     662 
   Size:       3419
 
DraI TTT'AAA

Cuts at:    418     418 
   Size:       3419
 
DrdI GACnn_nn'nnGTC

Cuts at:   1125    1983    1125 
   Size:        858    2561
 
EaeI y'GGCC_r

Cuts at:    134    1004    1178    1569    1596    2941     134 
   Size:        870     174     391      27    1345     612

  Fragments arranged by size: 

                1345     870     612     391     174      27
 
EagI C'GGCC_G

Cuts at:    134    1004     134 
   Size:        870    2549
 
EarI CTCTTCn'nnn_

Cuts at:    314     340     353    1442    1652     314 
   Size:         26      13    1089     210    2081

  Fragments arranged by size: 

                2081    1089     210      26      13
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1627    1937    2083    3229    1627 
   Size:        310     146    1146    1817

  Fragments arranged by size: 

                1817    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    431     431 
   Size:       3419
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    178     364    1244    1676    2423    2691     178 
   Size:        186     880     432     747     268     906

  Fragments arranged by size: 

                 906     880     747     432     268     186
 
EcoO109I rG'GnC_Cy

Cuts at:    153     334     558     153 
   Size:        181     224    3014

  Fragments arranged by size: 

                3014     224     181
 
EcoRI G'AATT_C

Cuts at:    423     423 
   Size:       3419
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1438    1438 
   Size:       3419
 
FspI TGC'GCA

Cuts at:   1200    1200 
   Size:       3419
 
HaeII r_GCGC'y

Cuts at:    149     853    1101    2123    3102     149 
   Size:        704     248    1022     979     466

  Fragments arranged by size: 

                1022     979     704     466     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1261    1291    1293    1323    1261 
   Size:         30       2      30    3357

  Fragments arranged by size: 

                3357      30      30       2
 
HincII GTy'rAC

Cuts at:    458     458 
   Size:       3419
 
KasI G'GCGC_C

Cuts at:    849    1097     849 
   Size:        248    3171
 
KpnI G_GTAC'C

Cuts at:    439     439 
   Size:       3419
 
MluI A'CGCG_T

Cuts at:   2852    2852 
   Size:       3419
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:     92    2090    2274      92 
   Size:       1998     184    1237

  Fragments arranged by size: 

                1998    1237     184
 
MscI TGG'CCA

Cuts at:   1180    1180 
   Size:       3419
 
MslI CAynn'nnrTG

Cuts at:   1408    1535    1408 
   Size:        127    3292
 
MspA1I CmG'CkG

Cuts at:    133     530     844    1204    2217    2462     133 
   Size:        397     314     360    1013     245    1090

  Fragments arranged by size: 

                1090    1013     397     360     314     245
 
NaeI GCC'GGC

Cuts at:   1600    1600 
   Size:       3419
 
NarI GG'CG_CC

Cuts at:    850    1098     850 
   Size:        248    3171
 
NgoMIV G'CCGG_C

Cuts at:   1598    1598 
   Size:       3419
 
NspI r_CATG'y

Cuts at:    472     972    1879     472 
   Size:        500     907    2012

  Fragments arranged by size: 

                2012     907     500
 
PciI A'CATG_T

Cuts at:   1875    1875 
   Size:       3419
 
PmeI GTTT'AAAC

Cuts at:    418     418 
   Size:       3419
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2558    2590    2558 
   Size:         32    3387
 
PpuMI rG'GwC_Cy

Cuts at:    153     153 
   Size:       3419
 
PspOMI G'GGCC_C

Cuts at:    334     334 
   Size:       3419
 
PstI C_TGCA'G

Cuts at:    466    1151     466 
   Size:        685    2734
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3419
 
PvuII CAG'CTG

Cuts at:    844    1204     844 
   Size:        360    3059
 
RsrII CG'GwC_CG

Cuts at:   1614    1614 
   Size:       3419
 
SacI G_AGCT'C

Cuts at:    433     433 
   Size:       3419
 
SalI G'TCGA_C

Cuts at:    456     456 
   Size:       3419
 
SapI GCTCTTCn'nnn_

Cuts at:   1442    1652    1442 
   Size:        210    3209
 
SbfI CC_TGCA'GG

Cuts at:    466     466 
   Size:       3419
 
ScaI AGT'ACT

Cuts at:   2936    2936 
   Size:       3419
 
SfcI C'TryA_G

Cuts at:     33     462    1147    2140    2331      33 
   Size:        429     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     429     191
 
SfoI GGC'GCC

Cuts at:    851    1099     851 
   Size:        248    3171
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3419
 
SmaI CCC'GGG

Cuts at:    441     441 
   Size:       3419
 
SmlI C'TyrA_G

Cuts at:    574    1489    1981    2243     574 
   Size:        915     492     262    1750

  Fragments arranged by size: 

                1750     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3419
 
SphI G_CATG'C

Cuts at:    472     972     472 
   Size:        500    2919
 
StyI C'CwwG_G

Cuts at:    149     553     907     149 
   Size:        404     354    2661

  Fragments arranged by size: 

                2661     404     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1002    1135    1594    1803    1002 
   Size:        133     459     209    2618

  Fragments arranged by size: 

                2618     459     209     133
 
TatI w'GTAC_w

Cuts at:   2934    2934 
   Size:       3419
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2936    2968    2936 
   Size:         32    3387
 
Tth111I GACn'n_nGTC

Cuts at:   1216    1216 
   Size:       3419
 
XbaI T'CTAG_A

Cuts at:    450     450 
   Size:       3419
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    914     914 
   Size:       3419
 
XmaI C'CCGG_G

Cuts at:    439     439 
   Size:       3419
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI     ApaI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI    BciVI     BclI    BglII     BlpI Bme1580I
     BmrI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BseRI    BseYI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstYI
    BtgZI     BtsI     DraI     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I
   HincII     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI   NgoMIV     NspI     PciI     PmeI     PpiI    PpuMI
   PspOMI     PstI     PvuI    PvuII    RsrII     SacI     SalI     SapI
     SbfI     ScaI     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI
     SphI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI
     AscI    AvrII     BaeI     BcgI    BfrBI     BglI    BmgBI     BmtI
     BpmI    BsaXI     BsgI    BsiWI     BsmI    BsmBI    BsrGI   BssHII
   BstEII    BstXI  BstZ17I   Bsu36I     BtgI     ClaI    CspCI   DraIII
    EcoNI    EcoRV     FseI    FspAI  HindIII     HpaI     MfeI     NcoI
     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI
     PfoI     PmlI    PshAI     PsiI    PspXI     PsrI    SacII    SanDI
    SexAI     SfiI    SgrAI    SnaBI     SrfI     SspI     StuI     SwaI
     XhoI     XmnI     ZraI
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