Restriction map for pF1KB8262
##################################
# NO CUT ENZYMES (  177 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AflII       AflIII      
AgeI        Alw44I      AlwNI       AocI        Aor51HI     
ApaI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspI        AvaII       
AviII       AvrII       BalI        BamHI       BanI        
BbeI        BbrPI       BbvCI       BcgI        BciVI       
BclI        BcnI        BfrI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BsaAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BsgI        
BsiEI       BsiWI       BsmAI       BsmBI       BsmI        
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrDI       BsrGI       BsrI        
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstXI       BstZ17I     
Bsu36I      CelII       CfoI        Cfr13I      ClaI        
CpoI        DraI        DraII       DraIII      DrdI        
DsaI        EagI        EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoO109I    EcoO65I     EcoRI       
EcoRV       EcoT22I     FauI        FbaI        FseI        
FspI        HaeII       HhaI        Hin1I       HinP1I      
HincII      HindII      HindIII     HpaI        KasI        
KpnI        Ksp632I     KspI        MamI        MfeI        
MluI        MluNI       MroI        MscI        MslI        
MunI        MvnI        NarI        NciI        NcoI        
NdeI        NheI        NlaIII      NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PvuI        PvuII       RcaI        RsrII       SacII       
SalI        SapI        Sau96I      SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        Tsp509I     
Tth111I     Van91I      XbaI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

AfaI           45  GTAC                 GT^AC
AhdI          313  GACATCAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI         313  GACATCAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI         187  GAGCTC               G[AT]GC[AT]^C
AvaI            9  CTCGGG               C^[CT]CG[GA]G
BfaI           85  CTAG                 C^TAG
BsiHKAI       187  GAGCTC               G[AT]GC[AT]^C
BsmFI         321  GTCCC                GGGAC(10/14)
BsoBI           9  CTCGGG               C^[CT]CG[GA]G
BsrFI         386  GCCGGC               [GA]^CCGG[CT]
Cfr10I        386  GCCGGC               [GA]^CCGG[CT]
EaeI           38  TGGCCG               [CT]^GGCC[GA]
Eam1105I      313  GACATCAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI          21  CCTGTCCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I        26  CCAAGG               C^C[AT][AT]GG
HapII         387  CCGG                 C^CGG
HgaI          436  GCGTC                GACGC(5/10)
HpaII         387  CCGG                 C^CGG
HphI          393  GGTGA                GGTGA(8/7)
MaeI           85  CTAG                 C^TAG
MseI          560  TTAA                 T^TAA
MspA1I        124  CAGCGG               C[AC]G^C[GT]G
MspI          387  CCGG                 C^CGG
MwoI          159  GCCCTTCGAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NaeI          386  GCCGGC               GCC^GGC
NgoMIV        386  GCCGGC               G^CCGGC
PstI          219  CTGCAG               CTGCA^G
RsaI           45  GTAC                 GT^AC
SacI          187  GAGCTC               GAGCT^C
SfaNI         338  GCATC                GCATC(5/9)
SfcI          219  CTGCAG               C^T[GA][CT]AG
SmlI          190  CTCAAG               C^T[CT][GA]AG
StyI           26  CCAAGG               C^C[AT][AT]GG
Tru9I         560  TTAA                 T^TAA
XcmI           26  CCAAGGAGCTGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AlwI           78  GATCC                GGATC(4/5)
              276  GGATC               
BanII           6  GGGCTC               G[GA]GC[CT]^C
              187  GAGCTC              
BbsI          376  GAAGAC               GAAGAC(2/6)
              226  GTCTTC              
BpmI          539  CTCCAG               CTGGAG(16/14)
              469  CTGGAG              
BpuAI         376  GAAGAC               GAAGAC(2/6)
              226  GTCTTC              
BsaJI          26  CCAAGG               C^C[ACGT][ACGT]GG
              116  CCCAGG              
BseRI         280  CTCCTC               GAGGAG(10/8)
              472  GAGGAG              
BstYI          77  AGATCC               [GA]^GATC[CT]
              276  GGATCT              
DpnII          78  GATC                 ^GATC
              277  GATC                
HaeIII         39  GGCC                 GG^CC
              195  GGCC                
MaeII          58  ACGT                 A^CGT
              524  ACGT                
MaeIII        141  GTCAC                ^GT[ACGT]AC
              296  GTGAC               
MboI           78  GATC                 ^GATC
              277  GATC                
MboII         376  GAAGA                GAAGA(8/7)
              227  TCTTC               
MflI           77  AGATCC               [GA]^GATC[CT]
              276  GGATCT              
NdeII          78  GATC                 ^GATC
              277  GATC                
NlaIV         361  GGAACC               GG[ACGT]^[ACGT]CC
              496  GGAACC              
PleI          136  GAGTC                GAGTC(4/5)
              475  GAGTC               
Sau3AI         78  GATC                 ^GATC
              277  GATC                
TaqI          164  TCGA                 T^CGA
              458  TCGA                
TfiI          171  GATTC                G^A[AT]TC
              215  GAATC               
Tsp45I        141  GTCAC                ^GT[GC]AC
              296  GTGAC               
TspRI         285  CAGTG                CA[GC]TG[ACGT][ACGT]^
              294  CAGTG               
TthHB8I       164  TCGA                 T^CGA
              458  TCGA                
XhoII          77  AGATCC               [GA]^GATC[CT]
              276  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   21 enzymes) #
#########################################

AciI        AluI        BbvI        BmyI        BsiYI       
BslI        Bsp1286I    BssKI       BstF5I      BstNI       
Cac8I       DdeI        EcoRII      Fnu4HI      FokI        
HinfI       ItaI        MnlI        MvaI        ScrFI       
TseI        
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