Restriction map for pF1KB8260
##################################
# NO CUT ENZYMES (  145 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AcsI        
AflII       AflIII      AgeI        AhdI        AocI        
Aor51HI     ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspI        AvaI        
AviII       AvrII       BalI        BbrPI       BbsI        
BcgI        BclI        BcnI        BfrI        BglII       
BlnI        BpmI        BpuAI       BsaAI       BsaBI       
BsaI        BsiEI       BsiWI       BsmAI       BsmBI       
BsmI        BsoBI       Bsp1407I    BspDI       BspHI       
BspLU11I    BspMI       BsrBI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstZ17I     
Bsu36I      ClaI        CpoI        DraI        DraIII      
EaeI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoRI       EcoRV       EcoT14I     
EcoT22I     FbaI        FseI        FspI        HincII      
HindII      HindIII     HpaI        KpnI        KspI        
MamI        MfeI        MluI        MluNI       MscI        
MseI        MspA1I      MunI        NaeI        NciI        
NcoI        NdeI        NgoMIV      NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PvuI        PvuII       RcaI        RsrII       SacI        
SacII       SalI        SbfI        SexAI       SfiI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
StyI        SwaI        TaqI        TfiI        Tru9I       
Tsp509I     Tth111I     TthHB8I     Van91I      XbaI        
XcmI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AccIII        110  TCCGGA               T^CCGGA
AfaI          396  GTAC                 GT^AC
Alw44I        292  GTGCAC               G^TGCAC
AlwNI         576  CAGCGACTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI           66  GGGCCC               GGGCC^C
ApaLI         292  GTGCAC               G^TGCAC
AvaII         498  GGACC                G^G[AT]CC
BamHI         148  GGATCC               G^GATCC
BanII          66  GGGCCC               G[GA]GC[CT]^C
BbeI          547  GGCGCC               GGCGC^C
BbvCI         351  CCTCAGC              CCTCAGC(-5/-2)
BciVI         116  GTATCC               GTATCC(6/5)
BfaI          439  CTAG                 C^TAG
BglI           26  GCCTTCCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI          300  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      300  GCTGAGC              GC^T[ACGT]AGC
BseAI         110  TCCGGA               T^CCGGA
BspEI         110  TCCGGA               T^CCGGA
BsrDI          84  CATTGC               GCAATG(2/0)
BsrFI         500  ACCGGC               [GA]^CCGG[CT]
BstEII        201  GGTCACC              G^GT[ACGT]ACC
BstPI         201  GGTCACC              G^GT[ACGT]ACC
BstXI         518  CCATGCTGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII         300  GCTGAGC              GC^T[ACGT]AGC
Cfr10I        500  ACCGGC               [GA]^CCGG[CT]
DraII          66  GGGCCCT              [GA]G^G[ACGT]CC[CT]
DrdI          187  GACTCGGTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI          507  CCACGG               C^C[GA][CT]GG
EarI           56  CTCTTC               CTCTTC(1/4)
EcoNI         420  CCTCAAAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I       66  GGGCCCT              [GA]G^G[ACGT]CC[CT]
EcoO65I       201  GGTCACC              G^GT[ACGT]ACC
FauI          488  CCCGC                CCCGC(4/6)
HaeII         547  GGCGCC               [GA]GCGC^[CT]
HphI          203  TCACC                GGTGA(8/7)
KasI          547  GGCGCC               G^GCGCC
Ksp632I        56  CTCTTC               CTCTTC(1/4)
MaeI          439  CTAG                 C^TAG
MaeII         161  ACGT                 A^CGT
MroI          110  TCCGGA               T^CCGGA
NarI          547  GGCGCC               GG^CGCC
NheI          438  GCTAGC               G^CTAGC
RsaI          396  GTAC                 GT^AC
SapI           55  GCTCTTC              GCTCTTC(1/4)
ScaI          395  AGTACT               AGT^ACT
SfaNI         139  GATGC                GCATC(5/9)
SfoI          547  GGCGCC               GGC^GCC
SmlI          343  CTCAAG               C^T[CT][GA]AG


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AcyI          415  GGCGTC               G[GA]^CG[CT]C
              547  GGCGCC              
AspHI         292  GTGCAC               G[AT]GC[AT]^C
              571  GAGCAC              
BsaHI         415  GGCGTC               G[GA]^CG[CT]C
              547  GGCGCC              
BsaWI          60  TCCGGT               [AT]^CCGG[AT]
              110  TCCGGA              
BseRI         349  CTCCTC               GAGGAG(10/8)
              427  GAGGAG              
BsiHKAI       292  GTGCAC               G[AT]GC[AT]^C
              571  GAGCAC              
BsmFI         328  GTCCC                GGGAC(10/14)
              367  GTCCC               
BstF5I        309  GGATG                GGATG(2/0)
              528  GGATG               
BstYI         148  GGATCC               [GA]^GATC[CT]
              251  AGATCC              
DpnII         149  GATC                 ^GATC
              252  GATC                
FokI          309  GGATG                GGATG(9/13)
              528  GGATG               
HgaI          416  GCGTC                GACGC(5/10)
              491  GCGTC               
Hin1I         415  GGCGTC               G[GA]^CG[CT]C
              547  GGCGCC              
MboI          149  GATC                 ^GATC
              252  GATC                
MboII         249  GAAGA                GAAGA(8/7)
               57  TCTTC               
MflI          148  GGATCC               [GA]^GATC[CT]
              251  AGATCC              
NdeII         149  GATC                 ^GATC
              252  GATC                
PstI            5  CTGCAG               CTGCA^G
               89  CTGCAG              
Sau3AI        149  GATC                 ^GATC
              252  GATC                
SfcI            5  CTGCAG               C^T[GA][CT]AG
               89  CTGCAG              
TspRI         290  CAGTG                CA[GC]TG[ACGT][ACGT]^
              456  CACTG               
XhoII         148  GGATCC               [GA]^GATC[CT]
              251  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   43 enzymes) #
#########################################

AccII       AciI        AluI        AlwI        BanI        
BbvI        BmyI        BsaJI       BsgI        BsiYI       
BslI        Bsp1286I    BsrI        BssKI       BstNI       
BstUI       Cac8I       CfoI        Cfr13I      DdeI        
EcoRII      Fnu4HI      HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       ItaI        MaeIII      
MnlI        MslI        MspI        MvaI        MvnI        
MwoI        NlaIII      NlaIV       PleI        Sau96I      
ScrFI       TseI        Tsp45I      
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com