Restriction map for pF1KB8260
 (Linear) (Six-base) MAPSORT of: KIBB8260.seq  Check: 2180  from: 1  to: 597



KIBB8260.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:04  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     268     392     597 
   Size:        268     124     205

  Fragments arranged by size: 

                 268     205     124
 
AleI CACnn'nnGTG

Cuts at:      0     466     597 
   Size:        466     131
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     484     535     597 
   Size:        484      51      62

  Fragments arranged by size: 

                 484      62      51
 
AlwNI CAG_nnn'CTG

Cuts at:      0     581     597 
   Size:        581      16
 
ApaI G_GGCC'C

Cuts at:      0      70     597 
   Size:         70     527
 
ApaLI G'TGCA_C

Cuts at:      0     292     597 
   Size:        292     305
 
BamHI G'GATC_C

Cuts at:      0     148     597 
   Size:        148     449
 
BanI G'GyrC_C

Cuts at:      0     321     360     460     503     547     597 
   Size:        321      39     100      43      44      50

  Fragments arranged by size: 

                 321     100      50      44      43      39
 
BanII G_rGCy'C

Cuts at:      0      70     597 
   Size:         70     527
 
BbeI G_GCGC'C

Cuts at:      0     551     597 
   Size:        551      46
 
BbvCI CC'TCA_GC

Cuts at:      0     352     597 
   Size:        352     245
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     127     597 
   Size:        127     470
 
BglI GCCn_nnn'nGGC

Cuts at:      0      32     597 
   Size:         32     565
 
BlpI GC'TnA_GC

Cuts at:      0     301     597 
   Size:        301     296
 
Bme1580I G_kGCm'C

Cuts at:      0      70     296     365     597 
   Size:         70     226      69     232

  Fragments arranged by size: 

                 232     226      70      69
 
BmgBI CAC'GTC

Cuts at:      0     162     597 
   Size:        162     435
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     283     597 
   Size:        283     314
 
BmtI G_CTAG'C

Cuts at:      0     442     597 
   Size:        442     155
 
Bpu10I CC'TnA_GC

Cuts at:      0     352     597 
   Size:        352     245
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     328     597 
   Size:        328     269
 
BsaHI Gr'CG_yC

Cuts at:      0     416     548     597 
   Size:        416     132      49

  Fragments arranged by size: 

                 416     132      49
 
BsaWI w'CCGG_w

Cuts at:      0      60     110     597 
   Size:         60      50     487

  Fragments arranged by size: 

                 487      60      50
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     340     442     597 
   Size:        340     102     155

  Fragments arranged by size: 

                 340     155     102
 
BseYI C'CCAG_C

Cuts at:      0     331     597 
   Size:        331     266
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     241     322     502     597 
   Size:        241      81     180      95

  Fragments arranged by size: 

                 241     180      95      81
 
BsiHKAI G_wGCw'C

Cuts at:      0     296     575     597 
   Size:        296     279      22

  Fragments arranged by size: 

                 296     279      22
 
Bsp1286I G_dGCh'C

Cuts at:      0      70     296     365     575     597 
   Size:         70     226      69     210      22

  Fragments arranged by size: 

                 226     210      70      69      22
 
BspEI T'CCGG_A

Cuts at:      0     110     597 
   Size:        110     487
 
BsrDI GCAATG_nn'

Cuts at:      0      83     597 
   Size:         83     514
 
BsrFI r'CCGG_y

Cuts at:      0     500     597 
   Size:        500      97
 
BstEII G'GTnAC_C

Cuts at:      0     201     597 
   Size:        201     396
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     525     597 
   Size:        525      72
 
BstYI r'GATC_y

Cuts at:      0     148     251     597 
   Size:        148     103     346

  Fragments arranged by size: 

                 346     148     103
 
BtgI C'CryG_G

Cuts at:      0     507     597 
   Size:        507      90
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     193     597 
   Size:        193     404
 
EarI CTCTTCn'nnn_

Cuts at:      0      62     597 
   Size:         62     535
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     268     392     597 
   Size:        268     124     205

  Fragments arranged by size: 

                 268     205     124
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     424     597 
   Size:        424     173
 
EcoO109I rG'GnC_Cy

Cuts at:      0      67     597 
   Size:         67     530
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     258     597 
   Size:        258     339
 
HaeII r_GCGC'y

Cuts at:      0     551     597 
   Size:        551      46
 
KasI G'GCGC_C

Cuts at:      0     547     597 
   Size:        547      50
 
MslI CAynn'nnrTG

Cuts at:      0     170     263     466     517     597 
   Size:        170      93     203      51      80

  Fragments arranged by size: 

                 203     170      93      80      51
 
NarI GG'CG_CC

Cuts at:      0     548     597 
   Size:        548      49
 
NheI G'CTAG_C

Cuts at:      0     438     597 
   Size:        438     159
 
PspOMI G'GGCC_C

Cuts at:      0      66     597 
   Size:         66     531
 
PstI C_TGCA'G

Cuts at:      0       9      93     597 
   Size:          9      84     504

  Fragments arranged by size: 

                 504      84       9
 
SapI GCTCTTCn'nnn_

Cuts at:      0      62     597 
   Size:         62     535
 
ScaI AGT'ACT

Cuts at:      0     397     597 
   Size:        397     200
 
SfcI C'TryA_G

Cuts at:      0       5      89     597 
   Size:          5      84     508

  Fragments arranged by size: 

                 508      84       5
 
SfoI GGC'GCC

Cuts at:      0     549     597 
   Size:        549      48
 
SmlI C'TyrA_G

Cuts at:      0     343     597 
   Size:        343     254
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     220     597 
   Size:        220     377
 
TatI w'GTAC_w

Cuts at:      0     395     597 
   Size:        395     202
 

 Enzymes that do cut:

     AcuI     AleI     AlfI    AlwNI     ApaI    ApaLI    BamHI     BanI
    BanII     BbeI    BbvCI    BciVI     BglI     BlpI Bme1580I    BmgBI
     BmrI     BmtI   Bpu10I    BpuEI    BsaHI    BsaWI    BseRI    BseYI
     BsgI  BsiHKAI Bsp1286I    BspEI    BsrDI    BsrFI   BstEII    BstXI
    BstYI     BtgI     DrdI     EarI  Eco57MI    EcoNI EcoO109I     FalI
    HaeII     KasI     MslI     NarI     NheI   PspOMI     PstI     SapI
     ScaI     SfcI     SfoI     SmlI    TaqII     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AloI     ApoI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI     BbsI     BcgI     BclI    BfrBI    BglII     BplI
     BpmI     BsaI    BsaAI    BsaBI    BsaXI    BsiEI    BsiWI     BsmI
    BsmBI    BspHI    BspMI    BsrBI    BsrGI   BssHII    BssSI   BstAPI
    BstBI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     EaeI     EagI     EciI  EcoICRI    EcoRI    EcoRV     FseI
     FspI    FspAI    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI
     MluI     MmeI     MscI   MspA1I     NaeI     NcoI     NdeI   NgoMIV
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI    PspXI
     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SbfI    SexAI     SfiI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     StyI     SwaI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI
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