Restriction map for pF1KB8254
##################################
# NO CUT ENZYMES (  173 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AfaI        AflII       
AflIII      AhdI        Alw44I      AlwNI       Aor51HI     
ApaI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspI        
AvaI        AvaII       AviII       AvrII       BalI        
BamHI       BanII       BbeI        BbrPI       BbsI        
BbvCI       BbvI        BcgI        BcnI        BfrI        
BglI        BglII       BlnI        BlpI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsaHI       BsaI        
BseAI       BseRI       BsgI        BsiWI       BsiYI       
BslI        BsmI        BsoBI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrGI       BsrI        BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstYI       BstZ17I     CelII       Cfr13I      
ClaI        CpoI        DraI        DraII       DraIII      
DrdI        EaeI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoNI       EcoO109I    EcoO65I     
EcoRI       EcoRV       FseI        FspI        HgaI        
Hin1I       HincII      HindII      HindIII     HpaI        
KasI        KpnI        KspI        MaeII       MamI        
MfeI        MflI        MluI        MluNI       MroI        
MscI        MunI        MvnI        NaeI        NarI        
NciI        NdeI        NgoMIV      NheI        NotI        
NruI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
RcaI        RsaI        RsrII       SacI        SacII       
SalI        SapI        Sau96I      SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        SwaI        TseI        Tsp45I      
Tth111I     Van91I      XbaI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   54 enzymes) #
###################################

AgeI          147  ACCGGT               A^CCGGT
AlwI          516  GATCC                GGATC(4/5)
AocI          295  CCTGAGG              CC^T[ACGT]AGG
AspHI         468  GTGCTC               G[AT]GC[AT]^C
BanI          305  GGCACC               G^G[CT][GA]CC
BclI           59  TGATCA               T^GATCA
BmyI          468  GTGCTC               G[AGT]GC[ACT]^C
BsaJI         431  CCATGG               C^C[ACGT][ACGT]GG
BsaWI         147  ACCGGT               [AT]^CCGG[AT]
BsiEI         374  CGATCG               CG[GA][CT]^CG
BsiHKAI       468  GTGCTC               G[AT]GC[AT]^C
BsmAI          29  GAGAC                GTCTC(1/5)
BsmBI          29  GAGACG               CGTCTC(1/5)
BsmFI         314  GTCCC                GGGAC(10/14)
Bsp1286I      468  GTGCTC               G[AGT]GC[ACT]^C
BsrFI         147  ACCGGT               [GA]^CCGG[CT]
BstXI         125  CCAATGTTATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        295  CCTGAGG              CC^T[ACGT]AGG
CfoI          475  GCGC                 GCG^C
Cfr10I        147  ACCGGT               [GA]^CCGG[CT]
DsaI          431  CCATGG               C^C[GA][CT]GG
Eco81I        295  CCTGAGG              CC^T[ACGT]AGG
EcoT14I       431  CCATGG               C^C[AT][AT]GG
EcoT22I        77  ATGCAT               ATGCA^T
FauI          534  CCCGC                CCCGC(4/6)
FbaI           59  TGATCA               T^GATCA
Fnu4HI        303  GCGGC                GC^[ACGT]GC
HaeII         474  GGCGCT               [GA]GCGC^[CT]
HapII         148  CCGG                 C^CGG
HhaI          475  GCGC                 GCG^C
HinP1I        475  GCGC                 G^CGC
HpaII         148  CCGG                 C^CGG
ItaI          303  GCGGC                GC^[ACGT]GC
MaeIII         65  GTTAC                ^GT[ACGT]AC
MseI          235  TTAA                 T^TAA
MslI          330  CATCCTAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        173  CAGCTG               C[AC]G^C[GT]G
MspI          148  CCGG                 C^CGG
NcoI          431  CCATGG               C^CATGG
NlaIII        432  CATG                 CATG^
NlaIV         305  GGCACC               GG[ACGT]^[ACGT]CC
NsiI           77  ATGCAT               ATGCA^T
PinAI         147  ACCGGT               A^CCGGT
PvuI          374  CGATCG               CGAT^CG
PvuII         173  CAGCTG               CAG^CTG
SfaNI         262  GCATC                GCATC(5/9)
SfcI           38  CTGTAG               C^T[GA][CT]AG
SmlI          493  CTCAAG               C^T[CT][GA]AG
StuI          489  AGGCCT               AGG^CCT
StyI          431  CCATGG               C^C[AT][AT]GG
TaqI          377  TCGA                 T^CGA
TfiI          153  GAATC                G^A[AT]TC
Tru9I         235  TTAA                 T^TAA
TthHB8I       377  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

BciVI         168  GGATAC               GTATCC(6/5)
              291  GTATCC              
BfaI          334  CTAG                 C^TAG
              427  CTAG                
BpmI           86  CTGGAG               CTGGAG(16/14)
              463  CTGGAG              
BssKI          85  CCTGG                ^CC[ACGT]GG
              462  CCTGG               
BstF5I        330  CATCC                GGATG(2/0)
              360  GGATG               
BstNI          85  CCTGG                CC^[AT]GG
              462  CCTGG               
EarI          549  GAAGAG               CTCTTC(1/4)
              555  GAAGAG              
EcoRII         85  CCTGG                ^CC[AT]GG
              462  CCTGG               
FokI          330  CATCC                GGATG(9/13)
              360  GGATG               
HaeIII          6  GGCC                 GG^CC
              490  GGCC                
HphI          150  GGTGA                GGTGA(8/7)
              410  TCACC               
Ksp632I       549  GAAGAG               CTCTTC(1/4)
              555  GAAGAG              
MaeI          334  CTAG                 C^TAG
              427  CTAG                
MvaI           85  CCTGG                CC^[AT]GG
              462  CCTGG               
MwoI          477  GCTCACACAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              514  GCGATCCGAGC         
PleI          402  GACTC                GAGTC(4/5)
              255  GAGTC               
ScrFI          85  CCTGG                CC^[ACGT]GG
              462  CCTGG               
Tsp509I       115  AATT                 ^AATT
              237  AATT                
TspRI         310  CACTG                CA[GC]TG[ACGT][ACGT]^
              500  CAGTG               
XcmI            8  CCATCAAGTGTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              326  CCATCATCCTAGTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   11 enzymes) #
#########################################

AciI        AluI        Cac8I       DdeI        DpnII       
HinfI       MboI        MboII       MnlI        NdeII       
Sau3AI      
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