Restriction map for pF1KB8246
##################################
# NO CUT ENZYMES (  146 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AcyI        
AflII       AgeI        AhdI        AlwI        AocI        
Aor51HI     ApaI        AscI        AseI        AsnI        
AspEI       AspI        AvaI        AviII       AvrII       
BalI        BamHI       BbeI        BbrPI       BbvCI       
BciVI       BclI        BcnI        BfrI        BglI        
BglII       BlnI        BlpI        BpmI        Bpu1102I    
BsaBI       BsaHI       BsaWI       BseAI       BsiWI       
BsmBI       BsmI        BsoBI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstYI       BstZ17I     Bsu36I      CelII       
ClaI        DraI        DraII       DraIII      DrdI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRI       
EcoRV       EcoT22I     FbaI        FseI        FspI        
HaeII       Hin1I       HincII      HindII      HindIII     
HpaI        KasI        KspI        MamI        MflI        
MluNI       MroI        MscI        MseI        MspA1I      
NarI        NciI        NcoI        NdeI        NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuII       RcaI        
SacII       SalI        SapI        SbfI        ScaI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        Sse8387I    SspBI       SspI        
StuI        SwaI        Tru9I       Tth111I     Van91I      
XbaI        XcmI        XhoI        XhoII       XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   60 enzymes) #
###################################

Acc65I        213  GGTACC               G^GTACC
AccII         150  CGCG                 CG^CG
AcsI          380  AAATTC               [GA]^AATT[CT]
AfaI          214  GTAC                 GT^AC
AflIII        149  ACGCGT               A^C[GA][CT]GT
Alw44I        145  GTGCAC               G^TGCAC
AlwNI         235  CAGGAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaLI         145  GTGCAC               G^TGCAC
ApoI          380  AAATTC               [GA]^AATT[CT]
Asp700I       376  GAAGAAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I       213  GGTACC               G^GTACC
BanI          213  GGTACC               G^G[CT][GA]CC
BanII         121  GAGCTC               G[GA]GC[CT]^C
BcgI          243  GCAACACATTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI         337  TACGTG               [CT]AC^GT[GA]
BsaI          205  GGTCTC               GGTCTC(1/5)
BseRI         292  GAGGAG               GAGGAG(10/8)
BsgI          274  GTGCAG               GTGCAG(16/14)
BsiEI          80  CGATCG               CG[GA][CT]^CG
BspMI         325  ACCTGC               ACCTGC(4/8)
BsrBI         295  GAGCGG               CCGCTC(-3/-3)
BsrFI         160  GCCGGC               [GA]^CCGG[CT]
BsrI          602  ACTGG                ACTGG(1/-1)
BstUI         150  CGCG                 CG^CG
BstXI         134  CCATCCCCGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI          180  GCGC                 GCG^C
Cfr10I        160  GCCGGC               [GA]^CCGG[CT]
CpoI            5  CGGTCCG              CG^G[AT]CCG
DsaI          140  CCGTGG               C^C[GA][CT]GG
EaeI          425  TGGCCG               [CT]^GGCC[GA]
EarI          524  CTCTTC               CTCTTC(1/4)
HapII         161  CCGG                 C^CGG
HgaI          151  GCGTC                GACGC(5/10)
HhaI          180  GCGC                 GCG^C
HinP1I        180  GCGC                 G^CGC
HinfI         712  GATTC                G^A[ACGT]TC
HpaII         161  CCGG                 C^CGG
KpnI          213  GGTACC               GGTAC^C
Ksp632I       524  CTCTTC               CTCTTC(1/4)
MaeII         338  ACGT                 A^CGT
MaeIII        319  GTCAC                ^GT[ACGT]AC
MfeI          497  CAATTG               C^AATTG
MluI          149  ACGCGT               A^CGCGT
MspI          161  CCGG                 C^CGG
MunI          497  CAATTG               C^AATTG
MvnI          150  CGCG                 CG^CG
NaeI          160  GCCGGC               GCC^GGC
NgoMIV        160  GCCGGC               G^CCGGC
NlaIV         213  GGTACC               GG[ACGT]^[ACGT]CC
PvuI           80  CGATCG               CGAT^CG
RsaI          214  GTAC                 GT^AC
RsrII           5  CGGTCCG              CG^G[AT]CCG
SacI          121  GAGCTC               GAGCT^C
SexAI         431  ACCAGGT              A^CC[AT]GGT
SfcI          102  CTACAG               C^T[GA][CT]AG
SmlI          397  CTCAAG               C^T[CT][GA]AG
SphI          704  GCATGC               GCATG^C
TfiI          712  GATTC                G^A[AT]TC
Tsp45I        319  GTCAC                ^GT[GC]AC
XmnI          376  GAAGAAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AvaII           6  GGTCC                G^G[AT]CC
              348  GGTCC               
BfaI          439  CTAG                 C^TAG
              686  CTAG                
BsmAI         370  GAGAC                GTCTC(1/5)
              206  GTCTC               
BsmFI         550  GGGAC                GGGAC(10/14)
              535  GTCCC               
Cfr13I          6  GGTCC                G^G[ACGT]CC
              348  GGTCC               
DdeI           90  CTCAG                C^T[ACGT]AG
              303  CTCAG               
DpnII          36  GATC                 ^GATC
               81  GATC                
EcoT14I       308  CCTTGG               C^C[AT][AT]GG
              344  CCTTGG              
MaeI          439  CTAG                 C^TAG
              686  CTAG                
MboI           36  GATC                 ^GATC
               81  GATC                
NdeII          36  GATC                 ^GATC
               81  GATC                
NlaIII        183  CATG                 CATG^
              705  CATG                
NspI          182  GCATGT               [GA]CATG^[CT]
              704  GCATGC              
Sau3AI         36  GATC                 ^GATC
               81  GATC                
Sau96I          6  GGTCC                G^G[ACGT]CC
              348  GGTCC               
SfaNI         170  GCATC                GCATC(5/9)
              483  GCATC               
StyI          308  CCTTGG               C^C[AT][AT]GG
              344  CCTTGG              
TaqI           20  TCGA                 T^CGA
              113  TCGA                
Tsp509I       381  AATT                 ^AATT
              498  AATT                
TthHB8I        20  TCGA                 T^CGA
              113  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   32 enzymes) #
#########################################

AciI        AluI        AspHI       BbsI        BbvI        
BmyI        BpuAI       BsaJI       BsiHKAI     BsiYI       
BslI        Bsp1286I    BsrDI       BssKI       BstF5I      
BstNI       Cac8I       EcoRII      FauI        Fnu4HI      
FokI        HaeIII      HphI        ItaI        MboII       
MnlI        MslI        MvaI        MwoI        ScrFI       
TseI        TspRI       
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