Restriction map for pF1KB8245
##################################
# NO CUT ENZYMES (  191 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AfaI        AflII       
AflIII      AgeI        Alw44I      AlwI        AlwNI       
AocI        Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp718I     AspHI       AspI        
AvaI        AvaII       AvrII       BamHI       BanI        
BanII       BbeI        BbrPI       BbsI        BbvCI       
BcgI        BciVI       BclI        BcnI        BfrI        
BglI        BglII       BlnI        BlpI        BmyI        
BpmI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BsgI        
BsiHKAI     BsiWI       BsmBI       BsmFI       BsmI        
BsoBI       Bsp1286I    Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstBI       BstEII      BstPI       BstUI       BstXI       
BstYI       BstZ17I     Bsu36I      CelII       Cfr10I      
ClaI        CpoI        DdeI        DraI        DraII       
DraIII      DrdI        DsaI        EagI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRV       EcoT14I     EcoT22I     FauI        
FbaI        FseI        HaeII       HapII       HgaI        
Hin1I       HincII      HindII      HindIII     HinfI       
HpaI        HpaII       KasI        KpnI        KspI        
MamI        MfeI        MflI        MluI        MroI        
MspA1I      MspI        MunI        MvnI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NlaIV       NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PvuII       RcaI        RsaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StyI        SwaI        
TaqI        TfiI        Tth111I     TthHB8I     Van91I      
XbaI        XcmI        XhoI        XhoII       XmaCI       
XmaI        


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# ONE CUT ENZYMES (   48 enzymes) #
###################################

AcsI          296  GAATTC               [GA]^AATT[CT]
AhdI          511  GACTGGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI          296  GAATTC               [GA]^AATT[CT]
Asp700I       292  GAAAGAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI         511  GACTGGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII          53  TGCGCA               TGC^GCA
BalI          401  TGGCCA               TGG^CCA
BfaI          427  CTAG                 C^TAG
BseRI          15  CTCCTC               GAGGAG(10/8)
BsiEI         285  CGATCG               CG[GA][CT]^CG
BsiYI         139  CCTACCATTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          139  CCTACCATTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI         438  GTCTC                GTCTC(1/5)
BssKI         255  CCAGG                ^CC[ACGT]GG
BstAPI        321  GCAGAAAATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI         255  CCAGG                CC^[AT]GG
Cac8I          82  GCTGGC               GC[ACGT]^[ACGT]GC
CfoI           54  GCGC                 GCG^C
Cfr13I          3  GGGCC                G^G[ACGT]CC
DpnII         286  GATC                 ^GATC
EaeI          401  TGGCCA               [CT]^GGCC[GA]
Eam1105I      511  GACTGGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI         296  GAATTC               G^AATTC
EcoRII        255  CCAGG                ^CC[AT]GG
FspI           53  TGCGCA               TGC^GCA
HhaI           54  GCGC                 GCG^C
HinP1I         54  GCGC                 G^CGC
HphI          128  TCACC                GGTGA(8/7)
MaeI          427  CTAG                 C^TAG
MaeII         537  ACGT                 A^CGT
MboI          286  GATC                 ^GATC
MluNI         401  TGGCCA               TGG^CCA
MscI          401  TGGCCA               TGG^CCA
MseI          152  TTAA                 T^TAA
MslI           57  CATTTTGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI          255  CCAGG                CC^[AT]GG
NdeII         286  GATC                 ^GATC
NlaIII        455  CATG                 CATG^
PstI          319  CTGCAG               CTGCA^G
PvuI          285  CGATCG               CGAT^CG
Sau3AI        286  GATC                 ^GATC
Sau96I          3  GGGCC                G^G[ACGT]CC
ScrFI         255  CCAGG                CC^[ACGT]GG
SfcI          319  CTGCAG               C^T[GA][CT]AG
StuI          223  AGGCCT               AGG^CCT
Tru9I         152  TTAA                 T^TAA
Tsp45I        127  GTCAC                ^GT[GC]AC
XmnI          292  GAAAGAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   11 enzymes) #
###################################

AluI          317  AGCT                 AG^CT
              527  AGCT                
BsaJI         254  CCCAGG               C^C[ACGT][ACGT]GG
              255  CCAGGG              
BsrI          490  ACTGG                ACTGG(1/-1)
              512  ACTGG               
BstF5I         75  GGATG                GGATG(2/0)
              198  GGATG               
EarI           22  CTCTTC               CTCTTC(1/4)
              440  CTCTTC              
FokI           75  GGATG                GGATG(9/13)
              198  GGATG               
Ksp632I        22  CTCTTC               CTCTTC(1/4)
              440  CTCTTC              
MaeIII        127  GTCAC                ^GT[ACGT]AC
              446  GTAAC               
MboII          23  TCTTC                GAAGA(8/7)
              441  TCTTC               
MwoI          321  GCAGAAAATGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              469  GCCACTTGTGC         
TspRI         356  CAGTG                CA[GC]TG[ACGT][ACGT]^
              408  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (    8 enzymes) #
#########################################

BbvI        Fnu4HI      HaeIII      ItaI        MnlI        
SfaNI       TseI        Tsp509I     
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