Restriction map for pF1KB8245
 (Circular) (Six-base) MAPSORT of: pF1KB8245.seq  Check: 8085  from: 1  to: 3653



pF1KB8245  3653 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:28  ..

AccI GT'mk_AC

Cuts at:    691     691 
   Size:       3653
 
Acc65I G'GTAC_C

Cuts at:    669     669 
   Size:       3653
 
AclI AA'CG_TT

Cuts at:   3493    3493 
   Size:       3653
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    238     526    1478    1910    2657    2925     238 
   Size:        288     952     432     747     268     966

  Fragments arranged by size: 

                 966     952     747     432     288     268
 
AflIII A'CryG_T

Cuts at:    645    1005    2109    3086     645 
   Size:        360    1104     977    1212

  Fragments arranged by size: 

                1212    1104     977     360
 
AgeI A'CCGG_T

Cuts at:   2041    2041 
   Size:       3653
 
AhdI GACnn_n'nnGTC

Cuts at:    624     624 
   Size:       3653
 
AlwNI CAG_nnn'CTG

Cuts at:    958    2525     958 
   Size:       1567    2086
 
ApaLI G'TGCA_C

Cuts at:   2423    2423 
   Size:       3653
 
ApoI r'AATT_y

Cuts at:    404     657    3006    3262     404 
   Size:        253    2349     256     795

  Fragments arranged by size: 

                2349     795     256     253
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3653
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3653
 
AvaI C'yCGr_G

Cuts at:    673     673 
   Size:       3653
 
BamHI G'GATC_C

Cuts at:    678     678 
   Size:       3653
 
BanI G'GyrC_C

Cuts at:    669    1083    1331    1366     669 
   Size:        414     248      35    2956

  Fragments arranged by size: 

                2956     414     248      35
 
BanII G_rGCy'C

Cuts at:    667    1697     667 
   Size:       1030    2623
 
BbeI G_GCGC'C

Cuts at:   1087    1335    1087 
   Size:        248    3405
 
BciVI GTATCCnnnnn_n'

Cuts at:   1551    2312    3653    1551 
   Size:        761    1341    1551

  Fragments arranged by size: 

                1551    1341     761
 
BclI T'GATC_A

Cuts at:   1173    1173 
   Size:       3653
 
BglII A'GATC_T

Cuts at:   1168    3205    1168 
   Size:       2037    1616
 
BlpI GC'TnA_GC

Cuts at:    765     765 
   Size:       3653
 
Bme1580I G_kGCm'C

Cuts at:   1278    1371    2427    1278 
   Size:         93    1056    2504

  Fragments arranged by size: 

                2504    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    865     946    1281    3425     865 
   Size:         81     335    2144    1093

  Fragments arranged by size: 

                2144    1093     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2020    2020 
   Size:       3653
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    829    1708    2200    2498     829 
   Size:        879     492     298    1984

  Fragments arranged by size: 

                1984     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3357    3357 
   Size:       3653
 
BsaAI yAC'GTr

Cuts at:   1006    1006 
   Size:       3653
 
BsaBI GATnn'nnATC

Cuts at:      6    1167    3204       6 
   Size:       1161    2037     455

  Fragments arranged by size: 

                2037    1161     455
 
BsaHI Gr'CG_yC

Cuts at:   1084    1332    2034    3530    1084 
   Size:        248     702    1496    1207

  Fragments arranged by size: 

                1496    1207     702     248
 
BsaWI w'CCGG_w

Cuts at:    842    1363    2041    2315    2462    2873     842 
   Size:        521     678     274     147     411    1622

  Fragments arranged by size: 

                1622     678     521     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    114     114 
   Size:       3653
 
BseYI C'CCAG_C

Cuts at:   1078    1836    2413    2909    1078 
   Size:        758     577     496    1822

  Fragments arranged by size: 

                1822     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104     396    1195    1241    2449     104 
   Size:        292     799      46    1208    1308

  Fragments arranged by size: 

                1308    1208     799     292      46
 
BsiHKAI G_wGCw'C

Cuts at:    667    1445    1635    2427     667 
   Size:        778     190     792    1893

  Fragments arranged by size: 

                1893     792     778     190
 
Bsp1286I G_dGCh'C

Cuts at:    667    1278    1371    1445    1635    1697    2427     667 
   Size:        611      93      74     190      62     730    1893

  Fragments arranged by size: 

                1893     730     611     190      93      74      62
 
BspEI T'CCGG_A

Cuts at:   2873    2873 
   Size:       3653
 
BspHI T'CATG_A

Cuts at:   2829    2829 
   Size:       3653
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    703     917    1219    1600     703 
   Size:        214     302     381    2756

  Fragments arranged by size: 

                2756     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    1945    1999    3483    3648      50 
   Size:       1895      54    1484     165      55

  Fragments arranged by size: 

                1895    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1565    1565 
   Size:       3653
 
BsrFI r'CCGG_y

Cuts at:   1651    1832    2041    1651 
   Size:        181     209    3263

  Fragments arranged by size: 

                3263     209     181
 
BssSI C'ACGA_G

Cuts at:   1924    2282    1924 
   Size:        358    3295
 
BstAPI GCAn_nnn'nTGC

Cuts at:    435    1030     435 
   Size:        595    3058
 
BstBI TT'CG_AA

Cuts at:   2014    2014 
   Size:       3653
 
BstYI r'GATC_y

Cuts at:      1     678    1145    1168    1503    1749    2761    2847    2859 
   Size:        677     467      23     335     246    1012      86      12

Cuts at:   2859    3205       1 
   Size:        346     449

  Fragments arranged by size: 

                1012     677     467     449     346     335     246      86
                  23      12
 
BtgI C'CryG_G

Cuts at:    107     107 
   Size:       3653
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1603    1784    1947    1603 
   Size:        181     163    3309

  Fragments arranged by size: 

                3309     181     163
 
BtsI GCAGTG_nn'

Cuts at:    470     896     470 
   Size:        426    3227
 
DraI TTT'AAA

Cuts at:    652     652 
   Size:       3653
 
DrdI GACnn_nn'nnGTC

Cuts at:   1359    2217    1359 
   Size:        858    2795
 
EaeI y'GGCC_r

Cuts at:    509    1238    1412    1803    1830    3175     509 
   Size:        729     174     391      27    1345     987

  Fragments arranged by size: 

                1345     987     729     391     174      27
 
EagI C'GGCC_G

Cuts at:   1238    1238 
   Size:       3653
 
EarI CTCTTCn'nnn_

Cuts at:    136     554    1676    1886     136 
   Size:        418    1122     210    1903

  Fragments arranged by size: 

                1903    1122     418     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1861    2171    2317    3463    1861 
   Size:        310     146    1146    2051

  Fragments arranged by size: 

                2051    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    665     665 
   Size:       3653
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    238     526    1478    1910    2657    2925     238 
   Size:        288     952     432     747     268     966

  Fragments arranged by size: 

                 966     952     747     432     288     268
 
EcoO109I rG'GnC_Cy

Cuts at:    792     792 
   Size:       3653
 
EcoRI G'AATT_C

Cuts at:    404     657     404 
   Size:        253    3400
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    366    1672     366 
   Size:       1306    2347
 
FspI TGC'GCA

Cuts at:    163    1434     163 
   Size:       1271    2382
 
FspAI rTGC'GCAy

Cuts at:    163     163 
   Size:       3653
 
HaeII r_GCGC'y

Cuts at:   1087    1335    2357    3336    1087 
   Size:        248    1022     979    1404

  Fragments arranged by size: 

                1404    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    610     642    1495    1525    1527    1557     610 
   Size:         32     853      30       2      30    2706

  Fragments arranged by size: 

                2706     853      32      30      30       2
 
HincII GTy'rAC

Cuts at:    692     692 
   Size:       3653
 
KasI G'GCGC_C

Cuts at:   1083    1331    1083 
   Size:        248    3405
 
KpnI G_GTAC'C

Cuts at:    673     673 
   Size:       3653
 
MluI A'CGCG_T

Cuts at:   3086    3086 
   Size:       3653
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    156    2324    2508     156 
   Size:       2168     184    1301

  Fragments arranged by size: 

                2168    1301     184
 
MscI TGG'CCA

Cuts at:    511    1414     511 
   Size:        903    2750
 
MslI CAynn'nnrTG

Cuts at:    169    1642    1769     169 
   Size:       1473     127    2053

  Fragments arranged by size: 

                2053    1473     127
 
MspA1I CmG'CkG

Cuts at:    764    1078    1438    2451    2696     764 
   Size:        314     360    1013     245    1721

  Fragments arranged by size: 

                1721    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   1834    1834 
   Size:       3653
 
NarI GG'CG_CC

Cuts at:   1084    1332    1084 
   Size:        248    3405
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       3653
 
NgoMIV G'CCGG_C

Cuts at:   1832    1832 
   Size:       3653
 
NspI r_CATG'y

Cuts at:    706    1206    2113     706 
   Size:        500     907    2246

  Fragments arranged by size: 

                2246     907     500
 
PasI CC'CwG_GG

Cuts at:    363     363 
   Size:       3653
 
PciI A'CATG_T

Cuts at:   2109    2109 
   Size:       3653
 
PmeI GTTT'AAAC

Cuts at:    652     652 
   Size:       3653
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2792    2824    2792 
   Size:         32    3621
 
PstI C_TGCA'G

Cuts at:    431     700    1385     431 
   Size:        269     685    2699

  Fragments arranged by size: 

                2699     685     269
 
PvuI CG_AT'CG

Cuts at:    104     396     104 
   Size:        292    3361
 
PvuII CAG'CTG

Cuts at:   1078    1438    1078 
   Size:        360    3293
 
RsrII CG'GwC_CG

Cuts at:   1848    1848 
   Size:       3653
 
SacI G_AGCT'C

Cuts at:    667     667 
   Size:       3653
 
SalI G'TCGA_C

Cuts at:    690     690 
   Size:       3653
 
SapI GCTCTTCn'nnn_

Cuts at:   1676    1886    1676 
   Size:        210    3443
 
SbfI CC_TGCA'GG

Cuts at:    700     700 
   Size:       3653
 
ScaI AGT'ACT

Cuts at:   3170    3170 
   Size:       3653
 
SfcI C'TryA_G

Cuts at:     33     427     696    1381    2374    2565      33 
   Size:        394     269     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     394     269     191
 
SfoI GGC'GCC

Cuts at:   1085    1333    1085 
   Size:        248    3405
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3653
 
SmaI CCC'GGG

Cuts at:    675     675 
   Size:       3653
 
SmlI C'TyrA_G

Cuts at:    808    1723    2215    2477     808 
   Size:        915     492     262    1984

  Fragments arranged by size: 

                1984     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3653
 
SphI G_CATG'C

Cuts at:    706    1206     706 
   Size:        500    3153
 
StuI AGG'CCT

Cuts at:    333     333 
   Size:       3653
 
StyI C'CwwG_G

Cuts at:    107     787    1141     107 
   Size:        680     354    2619

  Fragments arranged by size: 

                2619     680     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1236    1369    1828    2037    1236 
   Size:        133     459     209    2852

  Fragments arranged by size: 

                2852     459     209     133
 
TatI w'GTAC_w

Cuts at:   3168    3168 
   Size:       3653
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    228     260    3170    3202     228 
   Size:         32    2910      32     679

  Fragments arranged by size: 

                2910     679      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1450    1450 
   Size:       3653
 
XbaI T'CTAG_A

Cuts at:    684     684 
   Size:       3653
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1148    1148 
   Size:       3653
 
XmaI C'CCGG_G

Cuts at:    673     673 
   Size:       3653
 
XmnI GAAnn'nnTTC

Cuts at:    404     404 
   Size:       3653
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AhdI    AlwNI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI    BciVI     BclI    BglII     BlpI Bme1580I     BmrI     BplI
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseRI    BseYI
    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BssSI   BstAPI    BstBI    BstYI     BtgI    BtgZI     BtsI
     DraI     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
 EcoO109I    EcoRI     FalI     FspI    FspAI    HaeII    Hin4I   HincII
     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI   NgoMIV     NspI     PasI     PciI     PmeI     PpiI
     PstI     PvuI    PvuII    RsrII     SacI     SalI     SapI     SbfI
     ScaI     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI
     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AleI     AlfI     AloI     ApaI
     AscI    AvrII     BaeI     BbsI    BbvCI     BcgI    BfrBI     BglI
    BmgBI     BmtI     BpmI   Bpu10I    BsaXI     BsgI    BsiWI     BsmI
    BsmBI    BsrGI   BssHII   BstEII    BstXI  BstZ17I   Bsu36I     ClaI
    CspCI   DraIII    EcoNI    EcoRV     FseI  HindIII     HpaI     MfeI
     NdeI     NheI     NotI     NruI     NsiI     PacI    PflMI     PfoI
     PmlI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI    SacII
    SanDI    SexAI     SfiI    SgrAI    SnaBI     SrfI     SspI     SwaI
     XhoI     ZraI
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