Restriction map for pF1KB8240
##################################
# NO CUT ENZYMES (  175 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflIII      AgeI        AhdI        
Alw44I      AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AvaI        AvaII       AviII       
AvrII       BalI        BamHI       BanI        BbeI        
BbrPI       BbvCI       BcgI        BciVI       BcnI        
BfaI        BglI        BglII       BlnI        BlpI        
BpmI        Bpu1102I    BsaAI       BsaBI       BsaHI       
BsaI        BsaJI       BsaWI       BseAI       BsiEI       
BsiWI       BsmAI       BsmBI       BsmFI       BsmI        
BsoBI       Bsp1407I    BspDI       BspEI       BspLU11I    
BsrBI       BsrDI       BsrGI       BssHII      BssSI       
Bst1107I    BstBI       BstEII      BstPI       BstUI       
BstXI       BstYI       BstZ17I     Bsu36I      CelII       
ClaI        CpoI        DraI        DraII       DraIII      
DrdI        DsaI        EaeI        EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      Eco81I      
EcoO109I    EcoO65I     EcoRI       EcoRV       EcoT14I     
EcoT22I     FseI        FspI        Hin1I       HindIII     
HphI        KasI        KpnI        Ksp632I     KspI        
MaeI        MaeII       MaeIII      MamI        MfeI        
MflI        MluI        MluNI       MroI        MscI        
MslI        MspA1I      MunI        MvnI        NarI        
NciI        NcoI        NdeI        NheI        NlaIV       
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PinAI       PleI        
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RsrII       SacII       SalI        SapI        SbfI        
ScaI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        StyI        SwaI        
Tsp45I      TspRI       Tth111I     XbaI        XcmI        
XhoI        XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AciI           41  CCGC                 CCGC(-3/-1)
AfaI          307  GTAC                 GT^AC
AflII         457  CTTAAG               C^TTAAG
AlwI          694  GATCC                GGATC(4/5)
AlwNI         159  CAGCGACTG            CAG[ACGT][ACGT][ACGT]^CTG
AspHI         631  GAGCTC               G[AT]GC[AT]^C
BanII         631  GAGCTC               G[GA]GC[CT]^C
BclI          359  TGATCA               T^GATCA
BfrI          457  CTTAAG               C^TTAAG
BmyI          631  GAGCTC               G[AGT]GC[ACT]^C
BseRI         628  GAGGAG               GAGGAG(10/8)
BsgI          619  CTGCAC               GTGCAG(16/14)
BsiHKAI       631  GAGCTC               G[AT]GC[AT]^C
Bsp1286I      631  GAGCTC               G[AGT]GC[ACT]^C
BspHI         435  TCATGA               T^CATGA
BspMI         416  ACCTGC               ACCTGC(4/8)
BsrFI          43  GCCGGC               [GA]^CCGG[CT]
BssKI         447  CCTGG                ^CC[ACGT]GG
BstAPI        682  GCAATTATTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI         447  CCTGG                CC^[AT]GG
Cac8I          43  GCCGGC               GC[ACGT]^[ACGT]GC
CfoI          486  GCGC                 GCG^C
Cfr10I         43  GCCGGC               [GA]^CCGG[CT]
Cfr13I        167  GGCCC                G^G[ACGT]CC
EcoNI         489  CCTGACACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRII        447  CCTGG                ^CC[AT]GG
FauI           40  CCCGC                CCCGC(4/6)
FbaI          359  TGATCA               T^GATCA
HaeII         485  AGCGCC               [GA]GCGC^[CT]
HaeIII        167  GGCC                 GG^CC
HapII          44  CCGG                 C^CGG
HhaI          486  GCGC                 GCG^C
HinP1I        486  GCGC                 G^CGC
HpaI          199  GTTAAC               GTT^AAC
HpaII          44  CCGG                 C^CGG
MspI           44  CCGG                 C^CGG
MvaI          447  CCTGG                CC^[AT]GG
NaeI           43  GCCGGC               GCC^GGC
NgoMIV         43  GCCGGC               G^CCGGC
NlaIII        436  CATG                 CATG^
PflMI         708  CCACAGACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI          435  TCATGA               T^CATGA
RsaI          307  GTAC                 GT^AC
SacI          631  GAGCTC               GAGCT^C
Sau96I        167  GGCCC                G^G[ACGT]CC
ScrFI         447  CCTGG                CC^[ACGT]GG
SexAI         446  ACCTGGT              A^CC[AT]GGT
SfcI          643  CTACAG               C^T[GA][CT]AG
SmlI          457  CTTAAG               C^T[CT][GA]AG
Van91I        708  CCACAGACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

BbsI           97  GAAGAC               GAAGAC(2/6)
              127  GAAGAC              
BpuAI          97  GAAGAC               GAAGAC(2/6)
              127  GAAGAC              
BsiYI         489  CCTGACACAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              708  CCACAGACTGG         
BslI          489  CCTGACACAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              708  CCACAGACTGG         
HgaI            8  GACGC                GACGC(5/10)
              432  GCGTC               
HincII         15  GTTGAC               GT[CT]^[GA]AC
              199  GTTAAC              
HindII         15  GTTGAC               GT[CT]^[GA]AC
              199  GTTAAC              
HinfI         216  GATTC                G^A[ACGT]TC
              426  GATTC               
SfaNI         499  GATGC                GCATC(5/9)
              580  GATGC               
TaqI           26  TCGA                 T^CGA
              225  TCGA                
TfiI          216  GATTC                G^A[AT]TC
              426  GATTC               
TthHB8I        26  TCGA                 T^CGA
              225  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   21 enzymes) #
#########################################

AcsI        AluI        ApoI        BbvI        BsrI        
BstF5I      DdeI        DpnII       Fnu4HI      FokI        
ItaI        MboI        MboII       MnlI        MseI        
MwoI        NdeII       Sau3AI      Tru9I       TseI        
Tsp509I     
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