Restriction map for pF1KB8238
##################################
# NO CUT ENZYMES (  127 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AcsI        
AfaI        AflII       AflIII      AgeI        AhdI        
AocI        Aor51HI     ApaI        ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AviII       AvrII       BamHI       BanI        
BbeI        BbrPI       BbsI        BbvCI       BciVI       
BfrI        BglI        BlnI        BpuAI       BsaI        
BsiEI       BsiWI       BsmBI       BsmI        Bsp1407I    
BspDI       BspHI       BspLU11I    BsrDI       BsrFI       
BsrGI       BssHII      Bst1107I    BstBI       BstXI       
BstZ17I     Bsu36I      Cfr10I      ClaI        CpoI        
DraI        DraIII      DrdI        DsaI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoRI       EcoRV       EcoT14I     EcoT22I     FseI        
FspI        HaeII       HindIII     HpaI        KasI        
KpnI        KspI        MfeI        MluI        MunI        
NaeI        NarI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PshBI       Psp1406I    
PvuI        RcaI        RsaI        RsrII       SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StyI        SwaI        Tth111I     XbaI        
XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   55 enzymes) #
###################################

AccIII        134  TCCGGA               T^CCGGA
AcyI          874  GGCGTC               G[GA]^CG[CT]C
Alw44I         79  GTGCAC               G^TGCAC
ApaLI          79  GTGCAC               G^TGCAC
BalI          209  TGGCCA               TGG^CCA
BcgI          579  GCAGGTGACTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI          710  TGATCA               T^GATCA
BfaI          859  CTAG                 C^TAG
BglII         605  AGATCT               A^GATCT
BlpI          740  GCTCAGC              GC^T[ACGT]AGC
BpmI          787  CTGGAG               CTGGAG(16/14)
Bpu1102I      740  GCTCAGC              GC^T[ACGT]AGC
BsaAI         751  TACGTG               [CT]AC^GT[GA]
BsaBI         825  GATTGCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI         874  GGCGTC               G[GA]^CG[CT]C
BsaWI         134  TCCGGA               [AT]^CCGG[AT]
BseAI         134  TCCGGA               T^CCGGA
BsgI          658  CTGCAC               GTGCAG(16/14)
BsmAI          24  GAGAC                GTCTC(1/5)
BspEI         134  TCCGGA               T^CCGGA
BsrI          162  ACTGG                ACTGG(1/-1)
BssSI          16  CACGAG               CACGAG(-5/-1)
BstEII        198  GGTCACC              G^GT[ACGT]ACC
BstPI         198  GGTCACC              G^GT[ACGT]ACC
Cac8I         293  GCCTGC               GC[ACGT]^[ACGT]GC
CelII         740  GCTCAGC              GC^T[ACGT]AGC
EaeI          209  TGGCCA               [CT]^GGCC[GA]
EarI           49  GAAGAG               CTCTTC(1/4)
EcoNI         219  CCTTCCCGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       198  GGTCACC              G^GT[ACGT]ACC
FbaI          710  TGATCA               T^GATCA
HgaI          875  GCGTC                GACGC(5/10)
Hin1I         874  GGCGTC               G[GA]^CG[CT]C
HincII        352  GTCAAC               GT[CT]^[GA]AC
HindII        352  GTCAAC               GT[CT]^[GA]AC
Ksp632I        49  GAAGAG               CTCTTC(1/4)
MaeI          859  CTAG                 C^TAG
MamI          825  GATTGCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI         209  TGGCCA               TGG^CCA
MroI          134  TCCGGA               T^CCGGA
MscI          209  TGGCCA               TGG^CCA
MseI          598  TTAA                 T^TAA
PflMI         177  CCAGGAGTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          585  GACTC                GAGTC(4/5)
PshAI         150  GACTATTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SmaI           84  CCCGGG               CCC^GGG
StuI          593  AGGCCT               AGG^CCT
TaqI          588  TCGA                 T^CGA
TfiI          465  GATTC                G^A[AT]TC
Tru9I         598  TTAA                 T^TAA
TthHB8I       588  TCGA                 T^CGA
Van91I        177  CCAGGAGTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          488  CCAATGCTGGGATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI          84  CCCGGG               C^CCGGG
XmaI           84  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AccII          69  CGCG                 CG^CG
              259  CGCG                
AlwI          288  GATCC                GGATC(4/5)
              627  GATCC               
AvaI           84  CCCGGG               C^[CT]CG[GA]G
              223  CCCGAG              
BsmFI           5  GGGAC                GGGAC(10/14)
              390  GTCCC               
BsoBI          84  CCCGGG               C^[CT]CG[GA]G
              223  CCCGAG              
BsrBI          61  GAGCGG               CCGCTC(-3/-3)
              460  GAGCGG              
BstAPI        240  GCAGGAGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              249  GCAGAACCTGC         
BstUI          69  CGCG                 CG^CG
              259  CGCG                
CfoI           70  GCGC                 GCG^C
              258  GCGC                
DraII          10  AGGACCC              [GA]G^G[ACGT]CC[CT]
              383  GGGTCCT             
EcoO109I       10  AGGACCC              [GA]G^G[ACGT]CC[CT]
              383  GGGTCCT             
FauI            3  GCGGG                CCCGC(4/6)
               63  GCGGG               
HhaI           70  GCGC                 GCG^C
              258  GCGC                
HinP1I         70  GCGC                 G^CGC
              258  GCGC                
HinfI         465  GATTC                G^A[ACGT]TC
              585  GACTC               
MvnI           69  CGCG                 CG^CG
              259  CGCG                
NlaIII        234  CATG                 CATG^
              379  CATG                
PpuMI          10  AGGACCC              [GA]G^G[AT]CC[CT]
              383  GGGTCCT             
PstI          247  CTGCAG               CTGCA^G
              295  CTGCAG              
SacI          406  GAGCTC               GAGCT^C
              738  GAGCTC              
SfaNI         525  GATGC                GCATC(5/9)
              696  GATGC               
SfcI          247  CTGCAG               C^T[GA][CT]AG
              295  CTGCAG              


#########################################
# 3 OR MORE CUT ENZYMES (   54 enzymes) #
#########################################

AciI        AluI        AlwNI       AspHI       AvaII       
BanII       BbvI        BcnI        BmyI        BsaJI       
BseRI       BsiHKAI     BsiYI       BslI        Bsp1286I    
BspMI       BssKI       BstF5I      BstNI       BstYI       
Cfr13I      DdeI        DpnII       EcoRII      Fnu4HI      
FokI        HaeIII      HapII       HpaII       HphI        
ItaI        MaeII       MaeIII      MboI        MboII       
MflI        MnlI        MslI        MspA1I      MspI        
MvaI        MwoI        NciI        NdeII       NlaIV       
PvuII       Sau3AI      Sau96I      ScrFI       TseI        
Tsp45I      Tsp509I     TspRI       XhoII       
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