Restriction map for pF1KB8238
 (Linear) (Six-base) MAPSORT of: KIBB8238.seq  Check: 83  from: 1  to: 891



KIBB8238.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:04  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     570     891 
   Size:        570     321
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     322     499     589     891 
   Size:        322     177      90     302

  Fragments arranged by size: 

                 322     302     177      90
 
AleI CACnn'nnGTG

Cuts at:      0     580     891 
   Size:        580     311
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     445     891 
   Size:        445     446
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     103     135     331     363     891 
   Size:        103      32     196      32     528

  Fragments arranged by size: 

                 528     196     103      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     246     255     528     891 
   Size:        246       9     273     363

  Fragments arranged by size: 

                 363     273     246       9
 
ApaLI G'TGCA_C

Cuts at:      0      79     891 
   Size:         79     812
 
AvaI C'yCGr_G

Cuts at:      0      84     223     891 
   Size:         84     139     668

  Fragments arranged by size: 

                 668     139      84
 
BanII G_rGCy'C

Cuts at:      0     109     317     410     742     891 
   Size:        109     208      93     332     149

  Fragments arranged by size: 

                 332     208     149     109      93
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     568     602     891 
   Size:        568      34     289

  Fragments arranged by size: 

                 568     289      34
 
BclI T'GATC_A

Cuts at:      0     710     891 
   Size:        710     181
 
BglII A'GATC_T

Cuts at:      0     605     891 
   Size:        605     286
 
BlpI GC'TnA_GC

Cuts at:      0     741     891 
   Size:        741     150
 
Bme1580I G_kGCm'C

Cuts at:      0      83      91     891 
   Size:         83       8     800

  Fragments arranged by size: 

                 800      83       8
 
BmgBI CAC'GTC

Cuts at:      0     774     891 
   Size:        774     117
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     172     891 
   Size:        172     719
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     808     891 
   Size:        808      83
 
Bpu10I CC'TnA_GC

Cuts at:      0     736     891 
   Size:        736     155
 
BsaAI yAC'GTr

Cuts at:      0     753     891 
   Size:        753     138
 
BsaBI GATnn'nnATC

Cuts at:      0     829     891 
   Size:        829      62
 
BsaHI Gr'CG_yC

Cuts at:      0     875     891 
   Size:        875      16
 
BsaWI w'CCGG_w

Cuts at:      0     134     891 
   Size:        134     757
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     331     361     891 
   Size:        331      30     530

  Fragments arranged by size: 

                 530     331      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     130     241     526     891 
   Size:        130     111     285     365

  Fragments arranged by size: 

                 365     285     130     111
 
BseYI C'CCAG_C

Cuts at:      0      99     276     374     392     493     891 
   Size:         99     177      98      18     101     398

  Fragments arranged by size: 

                 398     177     101      99      98      18
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     643     891 
   Size:        643     248
 
BsiHKAI G_wGCw'C

Cuts at:      0      83     410     742     773     891 
   Size:         83     327     332      31     118

  Fragments arranged by size: 

                 332     327     118      83      31
 
Bsp1286I G_dGCh'C

Cuts at:      0      83      91     109     317     410     742     773     891 
   Size:         83       8      18     208      93     332      31     118

  Fragments arranged by size: 

                 332     208     118      93      83      31      18       8
            
 
BspEI T'CCGG_A

Cuts at:      0     134     891 
   Size:        134     757
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     186     263     570     891 
   Size:        186      77     307     321

  Fragments arranged by size: 

                 321     307     186      77
 
BsrBI CCG'CTC

Cuts at:      0      63     462     891 
   Size:         63     399     429

  Fragments arranged by size: 

                 429     399      63
 
BssSI C'ACGA_G

Cuts at:      0      16     891 
   Size:         16     875
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     246     255     891 
   Size:        246       9     636

  Fragments arranged by size: 

                 636     246       9
 
BstEII G'GTnAC_C

Cuts at:      0     198     891 
   Size:        198     693
 
BstYI r'GATC_y

Cuts at:      0     287     605     626     891 
   Size:        287     318      21     265

  Fragments arranged by size: 

                 318     287     265      21
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     275     891 
   Size:        275     616
 
EaeI y'GGCC_r

Cuts at:      0     209     891 
   Size:        209     682
 
EarI CTCTTCn'nnn_

Cuts at:      0      44     891 
   Size:         44     847
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     280     891 
   Size:        280     611
 
EcoICRI GAG'CTC

Cuts at:      0     408     740     891 
   Size:        408     332     151

  Fragments arranged by size: 

                 408     332     151
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     322     499     589     808     891 
   Size:        322     177      90     219      83

  Fragments arranged by size: 

                 322     219     177      90      83
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     223     891 
   Size:        223     668
 
EcoO109I rG'GnC_Cy

Cuts at:      0      11     384     891 
   Size:         11     373     507

  Fragments arranged by size: 

                 507     373      11
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     837     891 
   Size:        837      54
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      25      57     699     731     891 
   Size:         25      32     642      32     160

  Fragments arranged by size: 

                 642     160      32      32      25
 
HincII GTy'rAC

Cuts at:      0     354     891 
   Size:        354     537
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     164     497     867     891 
   Size:        164     333     370      24

  Fragments arranged by size: 

                 370     333     164      24
 
MscI TGG'CCA

Cuts at:      0     211     891 
   Size:        211     680
 
MslI CAynn'nnrTG

Cuts at:      0     361     580     835     891 
   Size:        361     219     255      56

  Fragments arranged by size: 

                 361     255     219      56
 
MspA1I CmG'CkG

Cuts at:      0     276     300     477     745     891 
   Size:        276      24     177     268     146

  Fragments arranged by size: 

                 276     268     177     146      24
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     183     891 
   Size:        183     708
 
PfoI T'CCnGG_A

Cuts at:      0     176     891 
   Size:        176     715
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     331     363     891 
   Size:        331      32     528

  Fragments arranged by size: 

                 528     331      32
 
PpuMI rG'GwC_Cy

Cuts at:      0      11     384     891 
   Size:         11     373     507

  Fragments arranged by size: 

                 507     373      11
 
PshAI GACnn'nnGTC

Cuts at:      0     154     891 
   Size:        154     737
 
PstI C_TGCA'G

Cuts at:      0     251     299     891 
   Size:        251      48     592

  Fragments arranged by size: 

                 592     251      48
 
PvuII CAG'CTG

Cuts at:      0     276     300     477     891 
   Size:        276      24     177     414

  Fragments arranged by size: 

                 414     276     177      24
 
SacI G_AGCT'C

Cuts at:      0     410     742     891 
   Size:        410     332     149

  Fragments arranged by size: 

                 410     332     149
 
SfcI C'TryA_G

Cuts at:      0     247     295     891 
   Size:        247      48     596

  Fragments arranged by size: 

                 596     247      48
 
SmaI CCC'GGG

Cuts at:      0      86     891 
   Size:         86     805
 
StuI AGG'CCT

Cuts at:      0     595     891 
   Size:        595     296
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     204     236     891 
   Size:        204      32     655

  Fragments arranged by size: 

                 655     204      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     495     891 
   Size:        495     396
 
XmaI C'CCGG_G

Cuts at:      0      84     891 
   Size:         84     807
 

 Enzymes that do cut:

     AarI     AcuI     AleI     AlfI     AloI    AlwNI    ApaLI     AvaI
    BanII     BcgI     BclI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BpmI   Bpu10I    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI  BsiHKAI Bsp1286I    BspEI    BspMI    BsrBI    BssSI
   BstAPI   BstEII    BstYI    BtgZI     EaeI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I     FalI    Hin4I   HincII     MmeI     MscI
     MslI   MspA1I    PflMI     PfoI     PpiI    PpuMI    PshAI     PstI
    PvuII     SacI     SfcI     SmaI     StuI     TstI     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     ApaI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI
    BamHI     BanI     BbeI     BbsI    BbvCI    BciVI    BfrBI     BglI
     BmtI     BplI    BpuEI     BsaI    BsiEI    BsiWI     BsmI    BsmBI
    BspHI    BsrDI    BsrFI    BsrGI   BssHII    BstBI    BstXI  BstZ17I
   Bsu36I     BtgI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI     PmeI     PmlI     PsiI   PspOMI    PspXI     PsrI
     PvuI    RsrII    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StyI     SwaI    TaqII     TatI  Tth111I
     XbaI     XhoI     XmnI     ZraI
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