Restriction map for pF1KB8224
##################################
# NO CUT ENZYMES (  143 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwI        AocI        Aor51HI     
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AviII       
AvrII       BamHI       BbrPI       BbvCI       BcgI        
BciVI       BclI        BfrI        BglII       BlnI        
BlpI        Bpu1102I    BsaAI       BsaBI       BsaI        
BsaWI       BseAI       BsiEI       BsiHKAI     BsiWI       
BsmI        Bsp1407I    BspDI       BspEI       BspLU11I    
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstBI       BstEII      BstPI       BstUI       BstYI       
BstZ17I     Bsu36I      CelII       Cfr10I      ClaI        
CpoI        DraI        DraIII      DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        HincII      HindII      
HindIII     HpaI        KpnI        KspI        MaeII       
MaeIII      MamI        MfeI        MflI        MluI        
MroI        MunI        MvnI        NaeI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        PvuII       RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfuI        SgrAI       SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        Tsp45I      XbaI        
XhoI        XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AfaI          243  GTAC                 GT^AC
AlwNI         749  CAGCCTCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI          556  GGGCCC               GGGCC^C
AspI          492  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BanI          215  GGCGCC               G^G[CT][GA]CC
BbeI          215  GGCGCC               GGCGC^C
BbsI          655  GAAGAC               GAAGAC(2/6)
BfaI          253  CTAG                 C^TAG
BglI          558  GCCCAGTGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI         655  GAAGAC               GAAGAC(2/6)
BseRI          46  GAGGAG               GAGGAG(10/8)
BsmBI         124  GAGACG               CGTCTC(1/5)
BspHI         359  TCATGA               T^CATGA
BspMI         279  GCAGGT               ACCTGC(4/8)
BsrBI         289  GAGCGG               CCGCTC(-3/-3)
BsrDI         461  GCAATG               GCAATG(2/0)
BstAPI        604  GCAGGAAATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI         410  CCATTGTGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DpnII         273  GATC                 ^GATC
EcoT14I        26  CCATGG               C^C[AT][AT]GG
HgaI          596  GACGC                GACGC(5/10)
KasI          215  GGCGCC               G^GCGCC
MaeI          253  CTAG                 C^TAG
MboI          273  GATC                 ^GATC
MspA1I        220  CAGCGG               C[AC]G^C[GT]G
NarI          215  GGCGCC               GG^CGCC
NcoI           26  CCATGG               C^CATGG
NdeII         273  GATC                 ^GATC
PflFI         492  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI         293  GGGTCCT              [GA]G^G[AT]CC[CT]
PstI          337  CTGCAG               CTGCA^G
RcaI          359  TCATGA               T^CATGA
RsaI          243  GTAC                 GT^AC
Sau3AI        273  GATC                 ^GATC
SfaNI         445  GATGC                GCATC(5/9)
SfcI          337  CTGCAG               C^T[GA][CT]AG
SfoI          215  GGCGCC               GGC^GCC
SmaI          200  CCCGGG               CCC^GGG
StyI           26  CCATGG               C^C[AT][AT]GG
TaqI          275  TCGA                 T^CGA
TfiI          688  GATTC                G^A[AT]TC
Tth111I       492  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I       275  TCGA                 T^CGA
XcmI           26  CCATGGCAGCCGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI         200  CCCGGG               C^CCGGG
XmaI          200  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AcyI          215  GGCGCC               G[GA]^CG[CT]C
              596  GACGCC              
AluI          119  AGCT                 AG^CT
              626  AGCT                
AvaI          200  CCCGGG               C^[CT]CG[GA]G
              704  CTCGGG              
BalI           99  TGGCCA               TGG^CCA
              740  TGGCCA              
BanII         166  GGGCTC               G[GA]GC[CT]^C
              556  GGGCCC              
BsaHI         215  GGCGCC               G[GA]^CG[CT]C
              596  GACGCC              
BsmAI         124  GAGAC                GTCTC(1/5)
              490  GAGAC               
BsmFI          22  GGGAC                GGGAC(10/14)
              498  GTCCC               
BsoBI         200  CCCGGG               C^[CT]CG[GA]G
              704  CTCGGG              
EaeI           99  TGGCCA               [CT]^GGCC[GA]
              740  TGGCCA              
Hin1I         215  GGCGCC               G[GA]^CG[CT]C
              596  GACGCC              
MluNI          99  TGGCCA               TGG^CCA
              740  TGGCCA              
MscI           99  TGGCCA               TGG^CCA
              740  TGGCCA              
MseI          229  TTAA                 T^TAA
              644  TTAA                
MslI          360  CATGAGAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              408  CACCATTGTG          
NlaIII         27  CATG                 CATG^
              360  CATG                
PflMI         102  CCACATCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              586  CCACGGGATGG         
Tru9I         229  TTAA                 T^TAA
              644  TTAA                
Tsp509I         5  AATT                 ^AATT
              227  AATT                
TspRI         494  CACTG                CA[GC]TG[ACGT][ACGT]^
              561  CAGTG               
Van91I        102  CCACATCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              586  CCACGGGATGG         


#########################################
# 3 OR MORE CUT ENZYMES (   48 enzymes) #
#########################################

AciI        AvaII       BbvI        BcnI        BmyI        
BpmI        BsaJI       BsgI        BsiYI       BslI        
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
Cac8I       CfoI        Cfr13I      DdeI        DraII       
DsaI        EarI        EcoO109I    EcoRII      FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MboII       MnlI        MspI        
MvaI        MwoI        NciI        NlaIV       PleI        
Sau96I      ScrFI       TseI        
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