Restriction map for pF1KB8224
 (Linear) (Six-base) MAPSORT of: KIBB8224.seq  Check: 82  from: 1  to: 759



KIBB8224.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:04  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     270     759 
   Size:        270     489
 
AleI CACnn'nnGTG

Cuts at:      0     412     759 
   Size:        412     347
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     337     338     369     370     759 
   Size:        337       1      31       1     389

  Fragments arranged by size: 

                 389     337      31       1       1
 
AlwNI CAG_nnn'CTG

Cuts at:      0     754     759 
   Size:        754       5
 
ApaI G_GGCC'C

Cuts at:      0     560     759 
   Size:        560     199
 
AvaI C'yCGr_G

Cuts at:      0     200     704     759 
   Size:        200     504      55

  Fragments arranged by size: 

                 504     200      55
 
BanI G'GyrC_C

Cuts at:      0     215     759 
   Size:        215     544
 
BanII G_rGCy'C

Cuts at:      0     170     560     759 
   Size:        170     390     199

  Fragments arranged by size: 

                 390     199     170
 
BbeI G_GCGC'C

Cuts at:      0     219     759 
   Size:        219     540
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     662     759 
   Size:        662      97
 
BglI GCCn_nnn'nGGC

Cuts at:      0     564     759 
   Size:        564     195
 
Bme1580I G_kGCm'C

Cuts at:      0     442     560     759 
   Size:        442     118     199

  Fragments arranged by size: 

                 442     199     118
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     554     759 
   Size:        554     205
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      69     344     759 
   Size:         69     275     415

  Fragments arranged by size: 

                 415     275      69
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     130     142     439     464     605     759 
   Size:        130      12     297      25     141     154

  Fragments arranged by size: 

                 297     154     141     130      25      12
 
Bpu10I CC'TnA_GC

Cuts at:      0     162     759 
   Size:        162     597
 
BsaHI Gr'CG_yC

Cuts at:      0     216     597     759 
   Size:        216     381     162

  Fragments arranged by size: 

                 381     216     162
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     127     157     759 
   Size:        127      30     602

  Fragments arranged by size: 

                 602     127      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      61     759 
   Size:         61     698
 
BseYI C'CCAG_C

Cuts at:      0     540     747     759 
   Size:        540     207      12

  Fragments arranged by size: 

                 540     207      12
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     337     503     658     759 
   Size:        337     166     155     101

  Fragments arranged by size: 

                 337     166     155     101
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     118     759 
   Size:        118     641
 
Bsp1286I G_dGCh'C

Cuts at:      0     170     442     560     759 
   Size:        170     272     118     199

  Fragments arranged by size: 

                 272     199     170     118
 
BspHI T'CATG_A

Cuts at:      0     359     759 
   Size:        359     400
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     270     759 
   Size:        270     489
 
BsrBI CCG'CTC

Cuts at:      0     291     759 
   Size:        291     468
 
BsrDI GCAATG_nn'

Cuts at:      0     468     759 
   Size:        468     291
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     610     759 
   Size:        610     149
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     417     759 
   Size:        417     342
 
BtgI C'CryG_G

Cuts at:      0      26      35     586     759 
   Size:         26       9     551     173

  Fragments arranged by size: 

                 551     173      26       9
 
EaeI y'GGCC_r

Cuts at:      0      99     740     759 
   Size:         99     641      19

  Fragments arranged by size: 

                 641      99      19
 
EarI CTCTTCn'nnn_

Cuts at:      0     179     185     565     759 
   Size:        179       6     380     194

  Fragments arranged by size: 

                 380     194     179       6
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     582     759 
   Size:        582     177
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     130     142     439     464     605     759 
   Size:        130      12     297      25     141     154

  Fragments arranged by size: 

                 297     154     141     130      25      12
 
EcoO109I rG'GnC_Cy

Cuts at:      0     294     556     681     759 
   Size:        294     262     125      78

  Fragments arranged by size: 

                 294     262     125      78
 
HaeII r_GCGC'y

Cuts at:      0      61     219     264     759 
   Size:         61     158      45     495

  Fragments arranged by size: 

                 495     158      61      45
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     127     159     481     513     759 
   Size:        127      32     322      32     246

  Fragments arranged by size: 

                 322     246     127      32      32
 
KasI G'GCGC_C

Cuts at:      0     215     759 
   Size:        215     544
 
MscI TGG'CCA

Cuts at:      0     101     742     759 
   Size:        101     641      17

  Fragments arranged by size: 

                 641     101      17
 
MslI CAynn'nnrTG

Cuts at:      0     364     412     759 
   Size:        364      48     347

  Fragments arranged by size: 

                 364     347      48
 
MspA1I CmG'CkG

Cuts at:      0     222     759 
   Size:        222     537
 
NarI GG'CG_CC

Cuts at:      0     216     759 
   Size:        216     543
 
NcoI C'CATG_G

Cuts at:      0      26     759 
   Size:         26     733
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     108     592     759 
   Size:        108     484     167

  Fragments arranged by size: 

                 484     167     108
 
PfoI T'CCnGG_A

Cuts at:      0     107     170     759 
   Size:        107      63     589

  Fragments arranged by size: 

                 589     107      63
 
PpuMI rG'GwC_Cy

Cuts at:      0     294     759 
   Size:        294     465
 
PspOMI G'GGCC_C

Cuts at:      0     556     759 
   Size:        556     203
 
PstI C_TGCA'G

Cuts at:      0     341     759 
   Size:        341     418
 
SfcI C'TryA_G

Cuts at:      0     337     759 
   Size:        337     422
 
SfoI GGC'GCC

Cuts at:      0     217     759 
   Size:        217     542
 
SmaI CCC'GGG

Cuts at:      0     202     759 
   Size:        202     557
 
StyI C'CwwG_G

Cuts at:      0      26     759 
   Size:         26     733
 
Tth111I GACn'n_nGTC

Cuts at:      0     495     759 
   Size:        495     264
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      33     759 
   Size:         33     726
 
XmaI C'CCGG_G

Cuts at:      0     200     759 
   Size:        200     559
 

 Enzymes that do cut:

     AarI     AleI     AloI    AlwNI     ApaI     AvaI     BanI    BanII
     BbeI     BbsI     BglI Bme1580I     BmrI     BplI     BpmI   Bpu10I
    BsaHI    BsaXI    BseRI    BseYI     BsgI    BsmBI Bsp1286I    BspHI
    BspMI    BsrBI    BsrDI   BstAPI    BstXI     BtgI     EaeI     EarI
     EciI  Eco57MI EcoO109I    HaeII    Hin4I     KasI     MscI     MslI
   MspA1I     NarI     NcoI    PflMI     PfoI    PpuMI   PspOMI     PstI
     SfcI     SfoI     SmaI     StyI  Tth111I     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI    ApaLI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI    BbvCI     BcgI    BciVI     BclI    BfrBI
    BglII     BlpI    BmgBI     BmtI    BpuEI     BsaI    BsaAI    BsaBI
    BsaWI    BsiEI  BsiHKAI    BsiWI     BsmI    BspEI    BsrFI    BsrGI
   BssHII    BssSI    BstBI   BstEII    BstYI  BstZ17I   Bsu36I    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EagI  EcoICRI
    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI   HincII
  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     NaeI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SgfI    SgrAI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII     TatI
     TstI     XbaI     XhoI     XmnI     ZraI
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