Restriction map for pF1KB8211
##################################
# NO CUT ENZYMES (  112 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AgeI        AhdI        Alw44I      
AlwNI       AocI        Aor51HI     ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AviII       AvrII       BbvCI       
BcgI        BciVI       BclI        BfrI        BglII       
BlnI        BlpI        Bpu1102I    BsaBI       BsaI        
BseAI       BsiWI       BsmAI       BsmBI       BsmI        
BspDI       BspEI       BsrDI       BssHII      Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstZ17I     
Bsu36I      CelII       ClaI        CpoI        DraI        
Eam1105I    Eco47III    Eco81I      EcoO65I     EcoRI       
EcoRV       EcoT22I     FbaI        FspI        HincII      
HindII      HindIII     HpaI        KpnI        MamI        
MfeI        MluI        MroI        MseI        MunI        
NdeI        NheI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmeI        PshAI       PshBI       Psp1406I    PvuI        
PvuII       RsrII       SacI        SalI        SapI        
SbfI        ScaI        SexAI       SfuI        SgrAI       
SmlI        SnaBI       SpeI        Sse8387I    SspI        
SwaI        Tru9I       Tth111I     Van91I      XbaI        
XhoI        XmnI        


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# ONE CUT ENZYMES (   45 enzymes) #
###################################

AflIII        797  ACATGT               A^C[GA][CT]GT
AspHI         244  GAGCAC               G[AT]GC[AT]^C
BalI          551  TGGCCA               TGG^CCA
BamHI         916  GGATCC               G^GATCC
BbrPI         907  CACGTG               CAC^GTG
BbsI          196  GTCTTC               GAAGAC(2/6)
BfaI          764  CTAG                 C^TAG
BpuAI         196  GTCTTC               GAAGAC(2/6)
BsaAI         907  CACGTG               [CT]AC^GT[GA]
BsaWI         718  ACCGGA               [AT]^CCGG[AT]
BsiHKAI       244  GAGCAC               G[AT]GC[AT]^C
Bsp1407I      800  TGTACA               T^GTACA
BspHI         809  TCATGA               T^CATGA
BspLU11I      797  ACATGT               A^CATGT
BspMI          83  ACCTGC               ACCTGC(4/8)
BsrBI         850  CCGCTC               CCGCTC(-3/-3)
BsrGI         800  TGTACA               T^GTACA
BsrI          671  CCAGT                ACTGG(1/-1)
DraIII        904  CACCACGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          117  GACCTTCCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI         430  CCTCCCGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FseI          972  GGCCGGCC             GGCCGG^CC
KspI         1000  CCGCGG               CCGC^GG
MaeI          764  CTAG                 C^TAG
MluNI         551  TGGCCA               TGG^CCA
MscI          551  TGGCCA               TGG^CCA
MspA1I       1000  CCGCGG               C[AC]G^C[GT]G
NotI         1021  GCGGCCGC             GC^GGCCGC
PleI          423  GAGTC                GAGTC(4/5)
PmaCI         907  CACGTG               CAC^GTG
PmlI          907  CACGTG               CAC^GTG
PstI          284  CTGCAG               CTGCA^G
RcaI          809  TCATGA               T^CATGA
SacII        1000  CCGCGG               CCGC^GG
SfiI          862  GGCCCGGAGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI          990  CCCGGG               CCC^GGG
SphI          584  GCATGC               GCATG^C
SspBI         800  TGTACA               T^GTACA
TaqI          682  TCGA                 T^CGA
TfiI          775  GATTC                G^A[AT]TC
Tsp509I       844  AATT                 ^AATT
TspRI         672  CAGTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I       682  TCGA                 T^CGA
XmaCI         990  CCCGGG               C^CCGGG
XmaI          990  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

BpmI          786  CTCCAG               CTGGAG(16/14)
              770  CTGGAG              
BsiEI          26  CGGCCG               CG[GA][CT]^CG
             1022  CGGCCG              
BssSI         488  CACGAG               CACGAG(-5/-1)
              542  CTCGTG              
BstXI         567  CCAGAGCTATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              629  CCATGAACATGG        
BstYI         131  AGATCC               [GA]^GATC[CT]
              916  GGATCC              
DdeI          128  CTGAG                C^T[ACGT]AG
              979  CTGAG               
EagI           26  CGGCCG               C^GGCCG
             1022  CGGCCG              
EarI          230  GAAGAG               CTCTTC(1/4)
              951  GAAGAG              
EclXI          26  CGGCCG               C^GGCCG
             1022  CGGCCG              
Eco52I         26  CGGCCG               C^GGCCG
             1022  CGGCCG              
HgaI          534  GACGC                GACGC(5/10)
              194  GCGTC               
HinfI         423  GAGTC                G^A[ACGT]TC
              775  GATTC               
Ksp632I       230  GAAGAG               CTCTTC(1/4)
              951  GAAGAG              
MaeII          71  ACGT                 A^CGT
              908  ACGT                
MflI          131  AGATCC               [GA]^GATC[CT]
              916  GGATCC              
NspI          584  GCATGC               [GA]CATG^[CT]
              797  ACATGT              
PpuMI         203  GGGACCT              [GA]G^G[AT]CC[CT]
             1030  GGGACCT             
SfaNI         417  GATGC                GCATC(5/9)
              501  GCATC               
SfcI          276  CTATAG               C^T[GA][CT]AG
              284  CTGCAG              
XcmI          296  CCAGCCCCGTTATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              884  CCATGAGCGCGATGG     
XhoII         131  AGATCC               [GA]^GATC[CT]
              916  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   80 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        ApaI        AvaI        AvaII       BanI        
BanII       BbeI        BbvI        BcnI        BglI        
BmyI        BsaHI       BsaJI       BseRI       BsgI        
BsiYI       BslI        BsmFI       BsoBI       Bsp1286I    
BsrFI       BssKI       BstF5I      BstNI       BstUI       
Cac8I       CfoI        Cfr10I      Cfr13I      DpnII       
DraII       DsaI        EaeI        EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HhaI        Hin1I       HinP1I      
HpaII       HphI        ItaI        KasI        MaeIII      
MboI        MboII       MnlI        MslI        MspI        
MvaI        MvnI        MwoI        NaeI        NarI        
NciI        NcoI        NdeII       NgoMIV      NlaIII      
NlaIV       RsaI        Sau3AI      Sau96I      ScrFI       
SfoI        StuI        StyI        TseI        Tsp45I      
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