Restriction map for pF1KB8211
 (Linear) (Six-base) MAPSORT of: KIBB8211.seq  Check: 425  from: 1  to: 1089



KIBB8211.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:03  ..

AflIII A'CryG_T

Cuts at:      0     797    1089 
   Size:        797     292
 
AleI CACnn'nnGTG

Cuts at:      0      95    1089 
   Size:         95     994
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     113    1089 
   Size:        113     976
 
ApaI G_GGCC'C

Cuts at:      0     400     566     643     743     874    1089 
   Size:        400     166      77     100     131     215

  Fragments arranged by size: 

                 400     215     166     131     100      77
 
AvaI C'yCGr_G

Cuts at:      0     927     990    1050    1089 
   Size:        927      63      60      39

  Fragments arranged by size: 

                 927      63      60      39
 
BamHI G'GATC_C

Cuts at:      0     916    1089 
   Size:        916     173
 
BanI G'GyrC_C

Cuts at:      0      97     219     469     829     964     994    1089 
   Size:         97     122     250     360     135      30      95

  Fragments arranged by size: 

                 360     250     135     122      97      95      30
 
BanII G_rGCy'C

Cuts at:      0     167     400     482     495     566     643     743     874 
   Size:        167     233      82      13      71      77     100     131

Cuts at:    874     928    1089 
   Size:         54     161

  Fragments arranged by size: 

                 233     167     161     131     100      82      77      71
                  54      13
 
BbeI G_GCGC'C

Cuts at:      0     223     473     833     968    1089 
   Size:        223     250     360     135     121

  Fragments arranged by size: 

                 360     250     223     135     121
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     189    1089 
   Size:        189     900
 
BglI GCCn_nnn'nGGC

Cuts at:      0     446     838     869    1089 
   Size:        446     392      31     220

  Fragments arranged by size: 

                 446     392     220      31
 
Bme1580I G_kGCm'C

Cuts at:      0      35     102     400     566     643     743     874     997 
   Size:         35      67     298     166      77     100     131     123

Cuts at:    997    1089 
   Size:         92

  Fragments arranged by size: 

                 298     166     131     123     100      92      77      67
                  35
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     665    1089 
   Size:        665     424
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     446    1089 
   Size:        446     643
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     771     791    1089 
   Size:        771      20     298

  Fragments arranged by size: 

                 771     298      20
 
Bpu10I CC'TnA_GC

Cuts at:      0     979    1089 
   Size:        979     110
 
BsaAI yAC'GTr

Cuts at:      0     909    1089 
   Size:        909     180
 
BsaHI Gr'CG_yC

Cuts at:      0     220     470     535     830     965    1089 
   Size:        220     250      65     295     135     124

  Fragments arranged by size: 

                 295     250     220     135     124      65
 
BsaWI w'CCGG_w

Cuts at:      0     718    1089 
   Size:        718     371
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     451     530     684     687     746    1089 
   Size:        451      79     154       3      59     343

  Fragments arranged by size: 

                 451     343     154      79      59       3
 
BseYI C'CCAG_C

Cuts at:      0     156     295     452    1089 
   Size:        156     139     157     637

  Fragments arranged by size: 

                 637     157     156     139
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      73     163    1024    1089 
   Size:         73      90     861      65

  Fragments arranged by size: 

                 861      90      73      65
 
BsiEI CG_ry'CG

Cuts at:      0      29    1025    1089 
   Size:         29     996      64

  Fragments arranged by size: 

                 996      64      29
 
BsiHKAI G_wGCw'C

Cuts at:      0     248    1089 
   Size:        248     841
 
Bsp1286I G_dGCh'C

Cuts at:      0      35     102     167     248     400     482     495     566 
   Size:         35      67      65      81     152      82      13      71

Cuts at:    566     643     743     874     928     997    1089 
   Size:         77     100     131      54      69      92

  Fragments arranged by size: 

                 152     131     100      92      82      81      77      71
                  69      67      65      54      35      13
 
BspHI T'CATG_A

Cuts at:      0     809    1089 
   Size:        809     280
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      92    1089 
   Size:         92     997
 
BsrBI CCG'CTC

Cuts at:      0     852    1089 
   Size:        852     237
 
BsrFI r'CCGG_y

Cuts at:      0     610     832     973    1089 
   Size:        610     222     141     116

  Fragments arranged by size: 

                 610     222     141     116
 
BsrGI T'GTAC_A

Cuts at:      0     800    1089 
   Size:        800     289
 
BssSI C'ACGA_G

Cuts at:      0     488     542    1089 
   Size:        488      54     547

  Fragments arranged by size: 

                 547     488      54
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     574     636    1089 
   Size:        574      62     453

  Fragments arranged by size: 

                 574     453      62
 
BstYI r'GATC_y

Cuts at:      0     131     916    1089 
   Size:        131     785     173

  Fragments arranged by size: 

                 785     173     131
 
BtgI C'CryG_G

Cuts at:      0     347     473     658     875     968    1000    1089 
   Size:        347     126     185     217      93      32      89

  Fragments arranged by size: 

                 347     217     185     126      93      89      32
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     907    1089 
   Size:        907     182
 
DraIII CAC_nnn'GTG

Cuts at:      0     909    1089 
   Size:        909     180
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     123    1089 
   Size:        123     966
 
EaeI y'GGCC_r

Cuts at:      0      26     335     551     878     971    1022    1089 
   Size:         26     309     216     327      93      51      67

  Fragments arranged by size: 

                 327     309     216      93      67      51      26
 
EagI C'GGCC_G

Cuts at:      0      26    1022    1089 
   Size:         26     996      67

  Fragments arranged by size: 

                 996      67      26
 
EarI CTCTTCn'nnn_

Cuts at:      0     225     946    1089 
   Size:        225     721     143

  Fragments arranged by size: 

                 721     225     143
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     771     791    1089 
   Size:        771      20     298

  Fragments arranged by size: 

                 771     298      20
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     434    1089 
   Size:        434     655
 
EcoO109I rG'GnC_Cy

Cuts at:      0     204     396     397     562     563     740     870     871 
   Size:        204     192       1     165       1     177     130       1

Cuts at:    871    1031    1089 
   Size:        160      58

  Fragments arranged by size: 

                 204     192     177     165     160     130      58       1
                   1       1
 
FseI GG_CCGG'CC

Cuts at:      0     977    1089 
   Size:        977     112
 
HaeII r_GCGC'y

Cuts at:      0      67     223     473     833     968    1089 
   Size:         67     156     250     360     135     121

  Fragments arranged by size: 

                 360     250     156     135     121      67
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     408     440     525     557     885     917    1089 
   Size:        408      32      85      32     328      32     172

  Fragments arranged by size: 

                 408     328     172      85      32      32      32
 
KasI G'GCGC_C

Cuts at:      0     219     469     829     964    1089 
   Size:        219     250     360     135     125

  Fragments arranged by size: 

                 360     250     219     135     125
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      62    1089 
   Size:         62    1027
 
MscI TGG'CCA

Cuts at:      0     553    1089 
   Size:        553     536
 
MslI CAynn'nnrTG

Cuts at:      0      95     634     808    1084    1089 
   Size:         95     539     174     276       5

  Fragments arranged by size: 

                 539     276     174      95       5
 
MspA1I CmG'CkG

Cuts at:      0    1002    1089 
   Size:       1002      87
 
NaeI GCC'GGC

Cuts at:      0     612     834     975    1089 
   Size:        612     222     141     114

  Fragments arranged by size: 

                 612     222     141     114
 
NarI GG'CG_CC

Cuts at:      0     220     470     830     965    1089 
   Size:        220     250     360     135     124

  Fragments arranged by size: 

                 360     250     220     135     124
 
NcoI C'CATG_G

Cuts at:      0     347     473     875    1089 
   Size:        347     126     402     214

  Fragments arranged by size: 

                 402     347     214     126
 
NgoMIV G'CCGG_C

Cuts at:      0     610     832     973    1089 
   Size:        610     222     141     116

  Fragments arranged by size: 

                 610     222     141     116
 
NotI GC'GGCC_GC

Cuts at:      0    1022    1089 
   Size:       1022      67
 
NspI r_CATG'y

Cuts at:      0     588     801    1089 
   Size:        588     213     288

  Fragments arranged by size: 

                 588     288     213
 
PasI CC'CwG_GG

Cuts at:      0     169     391     495     921    1089 
   Size:        169     222     104     426     168

  Fragments arranged by size: 

                 426     222     169     168     104
 
PciI A'CATG_T

Cuts at:      0     797    1089 
   Size:        797     292
 
PfoI T'CCnGG_A

Cuts at:      0     269    1089 
   Size:        269     820
 
PmlI CAC'GTG

Cuts at:      0     909    1089 
   Size:        909     180
 
PpuMI rG'GwC_Cy

Cuts at:      0     204    1031    1089 
   Size:        204     827      58

  Fragments arranged by size: 

                 827     204      58
 
PspOMI G'GGCC_C

Cuts at:      0     396     562     639     739     870    1089 
   Size:        396     166      77     100     131     219

  Fragments arranged by size: 

                 396     219     166     131     100      77
 
PstI C_TGCA'G

Cuts at:      0     288    1089 
   Size:        288     801
 
SacII CC_GC'GG

Cuts at:      0    1003    1089 
   Size:       1003      86
 
SfcI C'TryA_G

Cuts at:      0     276     284    1089 
   Size:        276       8     805

  Fragments arranged by size: 

                 805     276       8
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     869    1089 
   Size:        869     220
 
SfoI GGC'GCC

Cuts at:      0     221     471     831     966    1089 
   Size:        221     250     360     135     123

  Fragments arranged by size: 

                 360     250     221     135     123
 
SmaI CCC'GGG

Cuts at:      0     992    1089 
   Size:        992      97
 
SphI G_CATG'C

Cuts at:      0     588    1089 
   Size:        588     501
 
StuI AGG'CCT

Cuts at:      0     238     265     440     620    1089 
   Size:        238      27     175     180     469

  Fragments arranged by size: 

                 469     238     180     175      27
 
StyI C'CwwG_G

Cuts at:      0       8     260     347     473     875    1089 
   Size:          8     252      87     126     402     214

  Fragments arranged by size: 

                 402     252     214     126      87       8
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     612     768    1089 
   Size:        612     156     321

  Fragments arranged by size: 

                 612     321     156
 
TatI w'GTAC_w

Cuts at:      0     209     800    1089 
   Size:        209     591     289

  Fragments arranged by size: 

                 591     289     209
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     371     403    1089 
   Size:        371      32     686

  Fragments arranged by size: 

                 686     371      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     303     891    1089 
   Size:        303     588     198

  Fragments arranged by size: 

                 588     303     198
 
XmaI C'CCGG_G

Cuts at:      0     990    1089 
   Size:        990      99
 

 Enzymes that do cut:

   AflIII     AleI     AlfI     ApaI     AvaI    BamHI     BanI    BanII
     BbeI     BbsI     BglI Bme1580I     BmrI     BplI     BpmI   Bpu10I
    BsaAI    BsaHI    BsaWI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
 Bsp1286I    BspHI    BspMI    BsrBI    BsrFI    BsrGI    BssSI    BstXI
    BstYI     BtgI    BtgZI   DraIII     DrdI     EaeI     EagI     EarI
  Eco57MI    EcoNI EcoO109I     FseI    HaeII    Hin4I     KasI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NotI
     NspI     PasI     PciI     PfoI     PmlI    PpuMI   PspOMI     PstI
    SacII     SfcI     SfiI     SfoI     SmaI     SphI     StuI     StyI
    TaqII     TatI     TstI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AloI    AlwNI    ApaLI     ApoI     AscI     AseI
    AsiSI    AvrII     BaeI    BbvCI     BcgI    BciVI     BclI    BfrBI
    BglII     BlpI    BmgBI     BmtI    BpuEI     BsaI    BsaBI    BsaXI
    BsiWI     BsmI    BsmBI    BspEI    BsrDI   BssHII   BstAPI    BstBI
   BstEII  BstZ17I   Bsu36I     BtsI     ClaI    CspCI     DraI     EciI
  EcoICRI    EcoRI    EcoRV     FalI     FspI    FspAI   HincII  HindIII
     HpaI     KpnI     MfeI     MluI     NdeI     NheI     NruI     NsiI
     PacI    PflMI     PmeI     PpiI    PshAI     PsiI    PspXI     PsrI
     PvuI    PvuII    RsrII     SacI     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SgfI    SgrAI     SmlI    SnaBI     SpeI     SrfI
     SspI     SwaI  Tth111I     XbaI     XhoI     XmnI     ZraI
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