Restriction map for pF1KB8207
 (Linear) (Six-base) MAPSORT of: KIBB8207.seq  Check: 2251  from: 1  to: 876



KIBB8207.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:03  ..

AatII G_ACGT'C

Cuts at:      0     206     876 
   Size:        206     670
 
Acc65I G'GTAC_C

Cuts at:      0     497     876 
   Size:        497     379
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     193     280     400     820     876 
   Size:        193      87     120     420      56

  Fragments arranged by size: 

                 420     193     120      87      56
 
AflIII A'CryG_T

Cuts at:      0     551     876 
   Size:        551     325
 
AhdI GACnn_n'nnGTC

Cuts at:      0     555     876 
   Size:        555     321
 
AlwNI CAG_nnn'CTG

Cuts at:      0     258     393     876 
   Size:        258     135     483

  Fragments arranged by size: 

                 483     258     135
 
ApoI r'AATT_y

Cuts at:      0     241     876 
   Size:        241     635
 
AvaI C'yCGr_G

Cuts at:      0     443     876 
   Size:        443     433
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     481     514     876 
   Size:        481      33     362

  Fragments arranged by size: 

                 481     362      33
 
BanI G'GyrC_C

Cuts at:      0      37     497     876 
   Size:         37     460     379

  Fragments arranged by size: 

                 460     379      37
 
BbvCI CC'TCA_GC

Cuts at:      0     479     876 
   Size:        479     397
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     603     637     876 
   Size:        603      34     239

  Fragments arranged by size: 

                 603     239      34
 
BglII A'GATC_T

Cuts at:      0     152     876 
   Size:        152     724
 
Bme1580I G_kGCm'C

Cuts at:      0     758     876 
   Size:        758     118
 
BmgBI CAC'GTC

Cuts at:      0     542     876 
   Size:        542     334
 
Bpu10I CC'TnA_GC

Cuts at:      0     479     876 
   Size:        479     397
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     148     876 
   Size:        148     728
 
BsaHI Gr'CG_yC

Cuts at:      0     203     661     876 
   Size:        203     458     215

  Fragments arranged by size: 

                 458     215     203
 
BsaWI w'CCGG_w

Cuts at:      0     507     876 
   Size:        507     369
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     685     876 
   Size:        685     191
 
BseYI C'CCAG_C

Cuts at:      0     339     592     654     876 
   Size:        339     253      62     222

  Fragments arranged by size: 

                 339     253     222      62
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     193     876 
   Size:        193     683
 
Bsp1286I G_dGCh'C

Cuts at:      0     758     876 
   Size:        758     118
 
BspHI T'CATG_A

Cuts at:      0      78     198     876 
   Size:         78     120     678

  Fragments arranged by size: 

                 678     120      78
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     401     876 
   Size:        401     475
 
BsrDI GCAATG_nn'

Cuts at:      0     288     366     621     876 
   Size:        288      78     255     255

  Fragments arranged by size: 

                 288     255     255      78
 
BsrFI r'CCGG_y

Cuts at:      0     562     876 
   Size:        562     314
 
BstEII G'GTnAC_C

Cuts at:      0     286     876 
   Size:        286     590
 
BstYI r'GATC_y

Cuts at:      0     152     641     780     876 
   Size:        152     489     139      96

  Fragments arranged by size: 

                 489     152     139      96
 
BtsI GCAGTG_nn'

Cuts at:      0     682     876 
   Size:        682     194
 
DraIII CAC_nnn'GTG

Cuts at:      0     494     876 
   Size:        494     382
 
EaeI y'GGCC_r

Cuts at:      0     584     728     876 
   Size:        584     144     148

  Fragments arranged by size: 

                 584     148     144
 
EarI CTCTTCn'nnn_

Cuts at:      0     325     631     827     876 
   Size:        325     306     196      49

  Fragments arranged by size: 

                 325     306     196      49
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     129     860     876 
   Size:        129     731      16

  Fragments arranged by size: 

                 731     129      16
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     193     280     400     820     876 
   Size:        193      87     120     420      56

  Fragments arranged by size: 

                 420     193     120      87      56
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     163     876 
   Size:        163     713
 
EcoO109I rG'GnC_Cy

Cuts at:      0     255     375     837     876 
   Size:        255     120     462      39

  Fragments arranged by size: 

                 462     255     120      39
 
EcoRI G'AATT_C

Cuts at:      0     241     876 
   Size:        241     635
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     660     876 
   Size:        660     216
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      75     107     541     573     876 
   Size:         75      32     434      32     303

  Fragments arranged by size: 

                 434     303      75      32      32
 
KpnI G_GTAC'C

Cuts at:      0     501     876 
   Size:        501     375
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     672     876 
   Size:        672     204
 
MscI TGG'CCA

Cuts at:      0     586     876 
   Size:        586     290
 
MslI CAynn'nnrTG

Cuts at:      0     550     876 
   Size:        550     326
 
MspA1I CmG'CkG

Cuts at:      0     467     478     876 
   Size:        467      11     398

  Fragments arranged by size: 

                 467     398      11
 
NaeI GCC'GGC

Cuts at:      0     564     876 
   Size:        564     312
 
NgoMIV G'CCGG_C

Cuts at:      0     562     876 
   Size:        562     314
 
NspI r_CATG'y

Cuts at:      0     555     876 
   Size:        555     321
 
PciI A'CATG_T

Cuts at:      0     551     876 
   Size:        551     325
 
PfoI T'CCnGG_A

Cuts at:      0     146     876 
   Size:        146     730
 
PpuMI rG'GwC_Cy

Cuts at:      0     255     375     876 
   Size:        255     120     501

  Fragments arranged by size: 

                 501     255     120
 
PshAI GACnn'nnGTC

Cuts at:      0     866     876 
   Size:        866      10
 
PspXI vC'TCGA_Gb

Cuts at:      0     443     876 
   Size:        443     433
 
PstI C_TGCA'G

Cuts at:      0     791     845     876 
   Size:        791      54      31

  Fragments arranged by size: 

                 791      54      31
 
PvuII CAG'CTG

Cuts at:      0     478     876 
   Size:        478     398
 
SfcI C'TryA_G

Cuts at:      0     787     841     876 
   Size:        787      54      35

  Fragments arranged by size: 

                 787      54      35
 
SmlI C'TyrA_G

Cuts at:      0     163     443     876 
   Size:        163     280     433

  Fragments arranged by size: 

                 433     280     163
 
TatI w'GTAC_w

Cuts at:      0     533     876 
   Size:        533     343
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     497     529     876 
   Size:        497      32     347

  Fragments arranged by size: 

                 497     347      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     738     876 
   Size:        738     138
 
XhoI C'TCGA_G

Cuts at:      0     443     876 
   Size:        443     433
 
ZraI GAC'GTC

Cuts at:      0     204     876 
   Size:        204     672
 

 Enzymes that do cut:

    AatII   Acc65I     AcuI   AflIII     AhdI    AlwNI     ApoI     AvaI
     BaeI     BanI    BbvCI     BcgI    BglII Bme1580I    BmgBI   Bpu10I
    BpuEI    BsaHI    BsaWI    BseRI    BseYI     BsgI Bsp1286I    BspHI
    BspMI    BsrDI    BsrFI   BstEII    BstYI     BtsI   DraIII     EaeI
     EarI     EciI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI    Hin4I
     KpnI     MmeI     MscI     MslI   MspA1I     NaeI   NgoMIV     NspI
     PciI     PfoI    PpuMI    PshAI    PspXI     PstI    PvuII     SfcI
     SmlI     TatI     TstI     XcmI     XhoI     ZraI

 Enzymes that do not cut: 

     AarI     AccI     AclI     AfeI    AflII     AgeI     AleI     AlfI
     AloI     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII    BamHI
    BanII     BbeI     BbsI    BciVI     BclI    BfrBI     BglI     BlpI
     BmrI     BmtI     BplI     BpmI     BsaI    BsaAI    BsaBI    BsaXI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BsrBI    BsrGI
   BssHII    BssSI   BstAPI    BstBI    BstXI  BstZ17I   Bsu36I     BtgI
    BtgZI     ClaI    CspCI     DraI     DrdI     EagI  EcoICRI    EcoRV
     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI
     MfeI     MluI     NarI     NcoI     NdeI     NheI     NotI     NruI
     NsiI     PacI     PasI    PflMI     PmeI     PmlI     PpiI     PsiI
   PspOMI     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI    TaqII  Tth111I     XbaI     XmaI     XmnI
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