Restriction map for pF1KB8202
##################################
# NO CUT ENZYMES (  171 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AvaI        AviII       
AvrII       BalI        BanI        BbeI        BbrPI       
BbsI        BbvCI       BcgI        BciVI       BfaI        
BfrI        BglI        BglII       BlnI        BlpI        
BpmI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BsgI        
BsiEI       BsiHKAI     BsiWI       BsmAI       BsmBI       
BsmI        BsoBI       Bsp1407I    BspDI       BspEI       
BspLU11I    BsrDI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstXI       BstZ17I     Bsu36I      CelII       ClaI        
CpoI        DraI        DraIII      DrdI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoO65I     EcoRI       EcoRV       EcoT14I     
FseI        FspI        HaeII       HgaI        Hin1I       
HincII      HindII      HindIII     HpaI        KasI        
KpnI        Ksp632I     MaeI        MaeII       MamI        
MfeI        MluI        MluNI       MroI        MscI        
MseI        MslI        MunI        NarI        NcoI        
NdeI        NheI        NotI        NruI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PstI        PvuI        
PvuII       RsrII       SacI        SalI        SapI        
SbfI        ScaI        SexAI       SfcI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        StyI        SwaI        TaqI        
Tru9I       Tsp45I      Tth111I     TthHB8I     Van91I      
XbaI        XcmI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AfaI          548  GTAC                 GT^AC
AlwNI         173  CAGGAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
BamHI         555  GGATCC               G^GATCC
BanII          26  GGGCTC               G[GA]GC[CT]^C
BclI          248  TGATCA               T^GATCA
BcnI          118  CCCGG                CC^[GC]GG
BmyI           26  GGGCTC               G[AGT]GC[ACT]^C
BseRI         152  CTCCTC               GAGGAG(10/8)
Bsp1286I       26  GGGCTC               G[AGT]GC[ACT]^C
BspHI         200  TCATGA               T^CATGA
BspMI         332  ACCTGC               ACCTGC(4/8)
BsrBI         381  CCGCTC               CCGCTC(-3/-3)
BsrFI         448  GCCGGC               [GA]^CCGG[CT]
BstF5I        341  GGATG                GGATG(2/0)
BstYI         555  GGATCC               [GA]^GATC[CT]
CfoI          161  GCGC                 GCG^C
Cfr10I        448  GCCGGC               [GA]^CCGG[CT]
DraII         114  GGGACCC              [GA]G^G[ACGT]CC[CT]
EcoNI         427  CCTATCACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      114  GGGACCC              [GA]G^G[ACGT]CC[CT]
EcoT22I       496  ATGCAT               ATGCA^T
FbaI          248  TGATCA               T^GATCA
FokI          341  GGATG                GGATG(9/13)
HhaI          161  GCGC                 GCG^C
HinP1I        161  GCGC                 G^CGC
HinfI         243  GAATC                G^A[ACGT]TC
HphI          300  GGTGA                GGTGA(8/7)
KspI          140  CCGCGG               CCGC^GG
MaeIII        317  GTAAC                ^GT[ACGT]AC
MflI          555  GGATCC               [GA]^GATC[CT]
MspA1I        140  CCGCGG               C[AC]G^C[GT]G
NaeI          448  GCCGGC               GCC^GGC
NciI          118  CCCGG                CC^[GC]GG
NgoMIV        448  GCCGGC               G^CCGGC
NsiI          496  ATGCAT               ATGCA^T
PpuMI         114  GGGACCC              [GA]G^G[AT]CC[CT]
RcaI          200  TCATGA               T^CATGA
RsaI          548  GTAC                 GT^AC
SacII         140  CCGCGG               CCGC^GG
SfaNI         495  GATGC                GCATC(5/9)
TfiI          243  GAATC                G^A[AT]TC
XhoII         555  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

AccII         141  CGCG                 CG^CG
              162  CGCG                
AcsI            6  GAATTT               [GA]^AATT[CT]
              283  AAATTC              
AlwI          556  GATCC                GGATC(4/5)
              555  GGATC               
ApoI            6  GAATTT               [GA]^AATT[CT]
              283  AAATTC              
BbvI          236  GCAGC                GCAGC(8/12)
              374  GCAGC               
BsrI          466  ACTGG                ACTGG(1/-1)
              578  CCAGT               
BstUI         141  CGCG                 CG^CG
              162  CGCG                
DsaI          140  CCGCGG               C^C[GA][CT]GG
              146  CCACGG              
EaeI          143  CGGCCA               [CT]^GGCC[GA]
              446  TGGCCG              
MvnI          141  CGCG                 CG^CG
              162  CGCG                
TseI          236  GCAGC                G^C[AT]GC
              374  GCAGC               
Tsp509I         7  AATT                 ^AATT
              284  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (   33 enzymes) #
#########################################

AciI        AluI        AvaII       BsaJI       BsiYI       
BslI        BsmFI       BssKI       BstNI       Cac8I       
Cfr13I      DdeI        DpnII       EcoRII      FauI        
Fnu4HI      HaeIII      HapII       HpaII       ItaI        
MboI        MboII       MnlI        MspI        MvaI        
MwoI        NdeII       NlaIII      NlaIV       Sau3AI      
Sau96I      ScrFI       TspRI       
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