Restriction map for pF1KB8187
##################################
# NO CUT ENZYMES (  131 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AclI        
AcyI        AflII       AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaLI       AscI        AseI        
AsnI        AspEI       AvaI        AviII       AvrII       
BalI        BbeI        BbrPI       BbvCI       BciVI       
BfrI        BlnI        BsaAI       BsaHI       BsaI        
BsaWI       BseAI       BsiEI       BsiWI       BsmBI       
BsmI        BsoBI       BspDI       BspEI       BsrFI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstZ17I     Bsu36I      
CfoI        Cfr10I      ClaI        CpoI        DraI        
DrdI        EaeI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoO65I     EcoRI       
EcoRV       EcoT22I     FseI        FspI        HaeII       
HhaI        Hin1I       HinP1I      HincII      HindII      
HindIII     HpaI        KasI        KspI        MaeII       
MfeI        MluI        MluNI       MroI        MscI        
MunI        MvnI        NaeI        NarI        NcoI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflMI       PinAI       
PleI        PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        PvuII       RsrII       
SacI        SacII       SalI        SbfI        ScaI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspI        
SwaI        Van91I      XbaI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   57 enzymes) #
###################################

Acc65I       1167  GGTACC               G^GTACC
AcsI         1312  AAATTT               [GA]^AATT[CT]
AflIII       1121  ACATGT               A^C[GA][CT]GT
AlwNI         378  CAGCATCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI          368  GGGCCC               GGGCC^C
ApoI         1312  AAATTT               [GA]^AATT[CT]
Asp700I      1219  GAAGGCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      1167  GGTACC               G^GTACC
AspHI         748  GTGCTC               G[AT]GC[AT]^C
AspI          646  GACATTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BamHI         823  GGATCC               G^GATCC
BbvI          220  GCAGC                GCAGC(8/12)
BclI          158  TGATCA               T^GATCA
BglII        1277  AGATCT               A^GATCT
BlpI         1112  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     1112  GCTCAGC              GC^T[ACGT]AGC
BsaBI         153  GATGTTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI         785  CTCCTC               GAGGAG(10/8)
BsgI          310  CTGCAC               GTGCAG(16/14)
BsiHKAI       748  GTGCTC               G[AT]GC[AT]^C
Bsp1407I      246  TGTACA               T^GTACA
BspHI        1271  TCATGA               T^CATGA
BspLU11I     1121  ACATGT               A^CATGT
BsrDI         641  GCAATG               GCAATG(2/0)
BsrGI         246  TGTACA               T^GTACA
BstXI        1032  CCAATGTCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII        1112  GCTCAGC              GC^T[ACGT]AGC
DraIII         15  CACTCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI          192  CCGTGG               C^C[GA][CT]GG
EcoNI          78  CCTCAACCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I       419  CCAAGG               C^C[AT][AT]GG
FbaI          158  TGATCA               T^GATCA
HgaI         1109  GACGC                GACGC(5/10)
HphI         1155  GGTGA                GGTGA(8/7)
KpnI         1167  GGTACC               GGTAC^C
MamI          153  GATGTTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI          578  TTAA                 T^TAA
MspA1I        630  CCGCTG               C[AC]G^C[GT]G
NdeI          818  CATATG               CA^TATG
PflFI         646  GACATTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI         350  AGGACCT              [GA]G^G[AT]CC[CT]
PstI         1081  CTGCAG               CTGCA^G
RcaI         1271  TCATGA               T^CATGA
SapI          690  GAAGAGC              GCTCTTC(1/4)
SfcI         1081  CTGCAG               C^T[GA][CT]AG
SmlI         1336  CTTGAG               C^T[CT][GA]AG
SspBI         246  TGTACA               T^GTACA
StuI         1221  AGGCCT               AGG^CCT
StyI          419  CCAAGG               C^C[AT][AT]GG
TaqI          672  TCGA                 T^CGA
Tru9I         578  TTAA                 T^TAA
TseI          220  GCAGC                G^C[AT]GC
Tsp45I       1156  GTGAC                ^GT[GC]AC
Tth111I       646  GACATTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I       672  TCGA                 T^CGA
XcmI          854  CCAATTTCGTCGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI         1219  GAAGGCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

BanII         368  GGGCCC               G[GA]GC[CT]^C
              934  GAGCCC              
BbsI         1096  GAAGAC               GAAGAC(2/6)
             1291  GAAGAC              
BcgI           32  GCAACGCAGTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
              851  GCACCAATTTCG        
BcnI           24  CCGGG                CC^[GC]GG
              719  CCCGG               
BfaI          760  CTAG                 C^TAG
              931  CTAG                
BglI           23  GCCGGGAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              125  GCCGAGAGGGC         
BpmI          611  CTGGAG               CTGGAG(16/14)
              678  CTGGAG              
BpuAI        1096  GAAGAC               GAAGAC(2/6)
             1291  GAAGAC              
BsmAI         674  GAGAC                GTCTC(1/5)
              774  GTCTC               
BspMI         780  ACCTGC               ACCTGC(4/8)
             1056  ACCTGC              
BsrBI         135  CCGCTC               CCGCTC(-3/-3)
              448  GAGCGG              
BstYI         823  GGATCC               [GA]^GATC[CT]
             1277  AGATCT              
EarI          690  GAAGAG               CTCTTC(1/4)
              709  GAAGAG              
HapII          24  CCGG                 C^CGG
              720  CCGG                
HinfI         471  GAATC                G^A[ACGT]TC
              603  GAATC               
HpaII          24  CCGG                 C^CGG
              720  CCGG                
Ksp632I       690  GAAGAG               CTCTTC(1/4)
              709  GAAGAG              
MaeI          760  CTAG                 C^TAG
              931  CTAG                
MaeIII        386  GTAAC                ^GT[ACGT]AC
             1156  GTGAC               
MflI          823  GGATCC               [GA]^GATC[CT]
             1277  AGATCT              
MspI           24  CCGG                 C^CGG
              720  CCGG                
NciI           24  CCGGG                CC^[GC]GG
              719  CCCGG               
SexAI         353  ACCTGGT              A^CC[AT]GGT
             1242  ACCAGGT             
TfiI          471  GAATC                G^A[AT]TC
              603  GAATC               
XhoII         823  GGATCC               [GA]^GATC[CT]
             1277  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   45 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AvaII       
BanI        BmyI        BsaJI       BsiYI       BslI        
BsmFI       Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       Cac8I       Cfr13I      DdeI        DpnII       
DraII       EcoO109I    EcoRII      FauI        Fnu4HI      
FokI        HaeIII      ItaI        MboI        MboII       
MnlI        MslI        MvaI        MwoI        NdeII       
NlaIII      NlaIV       NspI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       Tsp509I     TspRI       
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