Restriction map for pF1KB8186
 (Linear) (Six-base) MAPSORT of: KIBB8186.seq  Check: 2959  from: 1  to: 909



KIBB8186.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:03  ..

AgeI A'CCGG_T

Cuts at:      0      82     909 
   Size:         82     827
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     763     909 
   Size:        763     146
 
AlwNI CAG_nnn'CTG

Cuts at:      0     771     909 
   Size:        771     138
 
ApaLI G'TGCA_C

Cuts at:      0     636     909 
   Size:        636     273
 
ApoI r'AATT_y

Cuts at:      0      40     187     260     812     909 
   Size:         40     147      73     552      97

  Fragments arranged by size: 

                 552     147      97      73      40
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     259     909 
   Size:        259     650
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     132     166     425     459     909 
   Size:        132      34     259      34     450

  Fragments arranged by size: 

                 450     259     132      34      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     140     909 
   Size:        140     769
 
Bme1580I G_kGCm'C

Cuts at:      0     389     640     909 
   Size:        389     251     269

  Fragments arranged by size: 

                 389     269     251
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     383     592     703     909 
   Size:        383     209     111     206

  Fragments arranged by size: 

                 383     209     206     111
 
Bpu10I CC'TnA_GC

Cuts at:      0     563     909 
   Size:        563     346
 
BsaAI yAC'GTr

Cuts at:      0     117     909 
   Size:        117     792
 
BsaWI w'CCGG_w

Cuts at:      0      82     909 
   Size:         82     827
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      37     422     443     909 
   Size:         37     385      21     466

  Fragments arranged by size: 

                 466     385      37      21
 
BseYI C'CCAG_C

Cuts at:      0     465     909 
   Size:        465     444
 
BsiHKAI G_wGCw'C

Cuts at:      0     640     909 
   Size:        640     269
 
BsiWI C'GTAC_G

Cuts at:      0     833     909 
   Size:        833      76
 
Bsp1286I G_dGCh'C

Cuts at:      0     389     640     909 
   Size:        389     251     269

  Fragments arranged by size: 

                 389     269     251
 
BspHI T'CATG_A

Cuts at:      0     420     909 
   Size:        420     489
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     770     909 
   Size:        770     139
 
BsrFI r'CCGG_y

Cuts at:      0      82     909 
   Size:         82     827
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     521     771     909 
   Size:        521     250     138

  Fragments arranged by size: 

                 521     250     138
 
BstYI r'GATC_y

Cuts at:      0     101     245     909 
   Size:        101     144     664

  Fragments arranged by size: 

                 664     144     101
 
BtgI C'CryG_G

Cuts at:      0       6     800     909 
   Size:          6     794     109

  Fragments arranged by size: 

                 794     109       6
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     395     909 
   Size:        395     514
 
EaeI y'GGCC_r

Cuts at:      0     776     909 
   Size:        776     133
 
HindIII A'AGCT_T

Cuts at:      0     454     909 
   Size:        454     455
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     651     909 
   Size:        651     258
 
MslI CAynn'nnrTG

Cuts at:      0     163     909 
   Size:        163     746
 
MspA1I CmG'CkG

Cuts at:      0       8      32     909 
   Size:          8      24     877

  Fragments arranged by size: 

                 877      24       8
 
NcoI C'CATG_G

Cuts at:      0     800     909 
   Size:        800     109
 
NdeI CA'TA_TG

Cuts at:      0     300     909 
   Size:        300     609
 
PfoI T'CCnGG_A

Cuts at:      0     323     909 
   Size:        323     586
 
PsiI TTA'TAA

Cuts at:      0     532     909 
   Size:        532     377
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     835     867     909 
   Size:        835      32      42

  Fragments arranged by size: 

                 835      42      32
 
PstI C_TGCA'G

Cuts at:      0     448     722     752     767     909 
   Size:        448     274      30      15     142

  Fragments arranged by size: 

                 448     274     142      30      15
 
PvuII CAG'CTG

Cuts at:      0      32     909 
   Size:         32     877
 
SacII CC_GC'GG

Cuts at:      0       9     909 
   Size:          9     900
 
SfcI C'TryA_G

Cuts at:      0     444     718     748     763     909 
   Size:        444     274      30      15     146

  Fragments arranged by size: 

                 444     274     146      30      15
 
SnaBI TAC'GTA

Cuts at:      0     117     909 
   Size:        117     792
 
SspI AAT'ATT

Cuts at:      0     122     909 
   Size:        122     787
 
StyI C'CwwG_G

Cuts at:      0      17     584     800     909 
   Size:         17     567     216     109

  Fragments arranged by size: 

                 567     216     109      17
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     224     909 
   Size:        224     685
 
TatI w'GTAC_w

Cuts at:      0     303     332     909 
   Size:        303      29     577

  Fragments arranged by size: 

                 577     303      29
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     798     909 
   Size:        798     111
 
XmnI GAAnn'nnTTC

Cuts at:      0      40     909 
   Size:         40     869
 

 Enzymes that do cut:

     AgeI     AlfI    AlwNI    ApaLI     ApoI     BbsI     BcgI     BglI
 Bme1580I     BmrI   Bpu10I    BsaAI    BsaWI    BseRI    BseYI  BsiHKAI
    BsiWI Bsp1286I    BspHI    BspMI    BsrFI   BstAPI    BstYI     BtgI
    BtgZI     EaeI  HindIII     MmeI     MslI   MspA1I     NcoI     NdeI
     PfoI     PsiI     PsrI     PstI    PvuII    SacII     SfcI    SnaBI
     SspI     StyI    TaqII     TatI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AhdI     AleI     AloI     ApaI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI    BbvCI
    BciVI     BclI    BfrBI    BglII     BlpI    BmgBI     BmtI     BplI
     BpmI    BpuEI     BsaI    BsaBI    BsaHI    BsaXI     BsgI    BsiEI
     BsmI    BsmBI    BspEI    BsrBI    BsrDI    BsrGI   BssHII    BssSI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EagI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII    Hin4I   HincII     HpaI     KasI     KpnI     MfeI     MluI
     MscI     NaeI     NarI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI
    PpuMI    PshAI   PspOMI    PspXI     PvuI    RsrII     SacI     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI     SpeI     SphI     SrfI     StuI     SwaI
     TstI  Tth111I     XbaI     XhoI     XmaI     ZraI
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