Restriction map for pF1KB8176
 (Linear) (Six-base) MAPSORT of: KIBB8176.seq  Check: 7344  from: 1  to: 735



KIBB8176.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:03  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      79     735 
   Size:         79     656
 
Acc65I G'GTAC_C

Cuts at:      0     380     735 
   Size:        380     355
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     410     735 
   Size:        410     325
 
AfeI AGC'GCT

Cuts at:      0      52     735 
   Size:         52     683
 
AleI CACnn'nnGTG

Cuts at:      0     286     735 
   Size:        286     449
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     304     735 
   Size:        304     431
 
BanI G'GyrC_C

Cuts at:      0      97     380     735 
   Size:         97     283     355

  Fragments arranged by size: 

                 355     283      97
 
BbeI G_GCGC'C

Cuts at:      0     101     735 
   Size:        101     634
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      13     208     339     629     735 
   Size:         13     195     131     290     106

  Fragments arranged by size: 

                 290     195     131     106      13
 
BglI GCCn_nnn'nGGC

Cuts at:      0      34      43     735 
   Size:         34       9     692

  Fragments arranged by size: 

                 692      34       9
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     195     735 
   Size:        195     540
 
BsaHI Gr'CG_yC

Cuts at:      0      98     735 
   Size:         98     637
 
BsaWI w'CCGG_w

Cuts at:      0     377     735 
   Size:        377     358
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     115     145     735 
   Size:        115      30     590

  Fragments arranged by size: 

                 590     115      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     130     735 
   Size:        130     605
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     264     549     716     735 
   Size:        264     285     167      19

  Fragments arranged by size: 

                 285     264     167      19
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      79     134     155     735 
   Size:         79      55      21     580

  Fragments arranged by size: 

                 580      79      55      21
 
BsrDI GCAATG_nn'

Cuts at:      0     596     735 
   Size:        596     139
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     291     735 
   Size:        291     444
 
BstYI r'GATC_y

Cuts at:      0     271     735 
   Size:        271     464
 
BtgI C'CryG_G

Cuts at:      0     134     284     735 
   Size:        134     150     451

  Fragments arranged by size: 

                 451     150     134
 
BtsI GCAGTG_nn'

Cuts at:      0      89     735 
   Size:         89     646
 
EaeI y'GGCC_r

Cuts at:      0      35      65     735 
   Size:         35      30     670

  Fragments arranged by size: 

                 670      35      30
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     159     487     735 
   Size:        159     328     248

  Fragments arranged by size: 

                 328     248     159
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     195     410     735 
   Size:        195     215     325

  Fragments arranged by size: 

                 325     215     195
 
HaeII r_GCGC'y

Cuts at:      0      54     101     735 
   Size:         54      47     634

  Fragments arranged by size: 

                 634      54      47
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     256     288     735 
   Size:        256      32     447

  Fragments arranged by size: 

                 447     256      32
 
KasI G'GCGC_C

Cuts at:      0      97     735 
   Size:         97     638
 
KpnI G_GTAC'C

Cuts at:      0     384     735 
   Size:        384     351
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     134     239     281     735 
   Size:        134     105      42     454

  Fragments arranged by size: 

                 454     134     105      42
 
MscI TGG'CCA

Cuts at:      0      67     735 
   Size:         67     668
 
MslI CAynn'nnrTG

Cuts at:      0      73     286     735 
   Size:         73     213     449

  Fragments arranged by size: 

                 449     213      73
 
MspA1I CmG'CkG

Cuts at:      0     231     735 
   Size:        231     504
 
NarI GG'CG_CC

Cuts at:      0      98     735 
   Size:         98     637
 
PvuII CAG'CTG

Cuts at:      0     231     735 
   Size:        231     504
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0      34      43     735 
   Size:         34       9     692

  Fragments arranged by size: 

                 692      34       9
 
SfoI GGC'GCC

Cuts at:      0      99     735 
   Size:         99     636
 
SspI AAT'ATT

Cuts at:      0     310     735 
   Size:        310     425
 
StyI C'CwwG_G

Cuts at:      0     435     735 
   Size:        435     300
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     103     220     506     735 
   Size:        103     117     286     229

  Fragments arranged by size: 

                 286     229     117     103
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     479     511     735 
   Size:        479      32     224

  Fragments arranged by size: 

                 479     224      32
 

 Enzymes that do cut:

     AarI   Acc65I     AcuI     AfeI     AleI     AlfI     BanI     BbeI
     BbsI     BglI     BpmI    BsaHI    BsaWI    BsaXI    BseRI     BsgI
    BspMI    BsrDI    BstXI    BstYI     BtgI     BtsI     EaeI     EciI
  Eco57MI    HaeII    Hin4I     KasI     KpnI     MmeI     MscI     MslI
   MspA1I     NarI    PvuII     SfiI     SfoI     SspI     StyI    TaqII
     TstI

 Enzymes that do not cut: 

    AatII     AccI     AclI    AflII   AflIII     AgeI     AhdI     AloI
    AlwNI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI    BamHI    BanII    BbvCI     BcgI    BciVI     BclI
    BfrBI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BseYI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BsrBI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I
    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EarI
  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI   HincII  HindIII     HpaI     MfeI     MluI     NaeI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     StuI     SwaI     TatI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI
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