Restriction map for pF1KB8173
##################################
# NO CUT ENZYMES (  130 enzymes) #
##################################

Acc65I      AccI        AccII       AccIII      AclI        
AcsI        AflII       AgeI        Alw44I      AocI        
Aor51HI     ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspHI       AvaI        
AviII       AvrII       BamHI       BanI        BbeI        
BbsI        BbvCI       BcgI        BciVI       BclI        
BfrI        BlnI        BlpI        Bpu1102I    BpuAI       
BsaWI       BseAI       BsiEI       BsiHKAI     BsiWI       
BsmI        BsoBI       BspEI       BspHI       BspLU11I    
BsrDI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstZ17I     
Bsu36I      CelII       CpoI        DraI        DraIII      
DrdI        DsaI        EagI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO65I     EcoRI       
EcoRV       EcoT22I     FbaI        FseI        FspI        
HaeII       HindIII     HpaI        KasI        KpnI        
KspI        MfeI        MluI        MroI        MunI        
MvnI        NaeI        NarI        NcoI        NdeI        
NgoMIV      NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PinAI       PleI        PmeI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacI        SacII       SalI        SbfI        
ScaI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspI        StuI        SwaI        Tsp509I     
XbaI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

AatII         292  GACGTC               GACGT^C
AcyI          292  GACGTC               G[GA]^CG[CT]C
AflIII       1005  ACACGT               A^C[GA][CT]GT
AhdI          597  GACCCCAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI           97  GGGCCC               GGGCC^C
AspEI         597  GACCCCAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI          463  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI          764  TGGCCA               TGG^CCA
BbrPI        1006  CACGTG               CAC^GTG
BfaI           65  CTAG                 C^TAG
BglI          962  GCCCAGAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         176  AGATCT               A^GATCT
BsaAI        1006  CACGTG               [CT]AC^GT[GA]
BsaBI         453  GATGCCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI         292  GACGTC               G[GA]^CG[CT]C
BsaI          563  GAGACC               GGTCTC(1/5)
BsgI          950  GTGCAG               GTGCAG(16/14)
Bsp1407I      585  TGTACA               T^GTACA
BspDI         922  ATCGAT               AT^CGAT
BsrBI         190  CCGCTC               CCGCTC(-3/-3)
BsrFI         113  ACCGGC               [GA]^CCGG[CT]
BsrGI         585  TGTACA               T^GTACA
BstXI         458  CCATCGACCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI          844  GCGC                 GCG^C
Cfr10I        113  ACCGGC               [GA]^CCGG[CT]
ClaI          922  ATCGAT               AT^CGAT
DdeI          353  CTGAG                C^T[ACGT]AG
Eam1105I      597  GACCCCAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HhaI          844  GCGC                 GCG^C
Hin1I         292  GACGTC               G[GA]^CG[CT]C
HinP1I        844  GCGC                 G^CGC
HincII        874  GTCAAC               GT[CT]^[GA]AC
HindII        874  GTCAAC               GT[CT]^[GA]AC
MaeI           65  CTAG                 C^TAG
MamI          453  GATGCCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI         764  TGGCCA               TGG^CCA
MscI          764  TGGCCA               TGG^CCA
MseI          890  TTAA                 T^TAA
MspA1I        838  CAGCTG               C[AC]G^C[GT]G
NheI           64  GCTAGC               G^CTAGC
NspI          238  ACATGC               [GA]CATG^[CT]
PflFI         463  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI        1006  CACGTG               CAC^GTG
PmlI         1006  CACGTG               CAC^GTG
PpuMI         595  GGGACCC              [GA]G^G[AT]CC[CT]
PstI          492  CTGCAG               CTGCA^G
PvuII         838  CAGCTG               CAG^CTG
SapI          192  GCTCTTC              GCTCTTC(1/4)
SexAI         464  ACCTGGT              A^CC[AT]GGT
SfcI          492  CTGCAG               C^T[GA][CT]AG
SspBI         585  TGTACA               T^GTACA
Tru9I         890  TTAA                 T^TAA
Tth111I       463  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AlwNI         124  CAGCCCCTG            CAG[ACGT][ACGT][ACGT]^CTG
              412  CAGGTGCTG           
BanII          97  GGGCCC               G[GA]GC[CT]^C
              856  GGGCTC              
BspMI         428  ACCTGC               ACCTGC(4/8)
              342  GCAGGT              
BstYI         176  AGATCT               [GA]^GATC[CT]
              749  GGATCT              
EaeI          764  TGGCCA               [CT]^GGCC[GA]
             1010  TGGCCG              
EcoT14I       654  CCAAGG               C^C[AT][AT]GG
              758  CCAAGG              
HgaI          902  GACGC                GACGC(5/10)
              996  GACGC               
HinfI         307  GATTC                G^A[ACGT]TC
              558  GATTC               
HphI          289  GGTGA                GGTGA(8/7)
              860  TCACC               
MaeII         293  ACGT                 A^CGT
             1007  ACGT                
MflI          176  AGATCT               [GA]^GATC[CT]
              749  GGATCT              
MslI          408  CATCCAGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              672  CACCAGCGTG          
PflMI         426  CCACCTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              607  CCATCCAGTGG         
StyI          654  CCAAGG               C^C[AT][AT]GG
              758  CCAAGG              
TfiI          307  GATTC                G^A[AT]TC
              558  GATTC               
Van91I        426  CCACCTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              607  CCATCCAGTGG         
XhoII         176  AGATCT               [GA]^GATC[CT]
              749  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   58 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AvaII       
BbvI        BcnI        BmyI        BpmI        BsaJI       
BseRI       BsiYI       BslI        BsmAI       BsmBI       
BsmFI       Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       Cac8I       Cfr13I      DpnII       DraII       
EarI        EcoO109I    EcoRII      FauI        Fnu4HI      
FokI        HaeIII      HapII       HpaII       ItaI        
Ksp632I     MaeIII      MboI        MboII       MnlI        
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       TaqI        TseI        Tsp45I      
TspRI       TthHB8I     XcmI        
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