Restriction map for pF1KB8173
 (Circular) (Six-base) MAPSORT of: pF1KB8173.seq  Check: 7179  from: 1  to: 4187



pF1KB8173  4187 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:27  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    545     545 
   Size:       4187
 
AatII G_ACGT'C

Cuts at:    404     404 
   Size:       4187
 
AccI GT'mk_AC

Cuts at:   1225    1225 
   Size:       4187
 
Acc65I G'GTAC_C

Cuts at:   1203    1203 
   Size:       4187
 
AclI AA'CG_TT

Cuts at:   4027    4027 
   Size:       4187
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    286    1050    2012    2444    3191    3459     286 
   Size:        764     962     432     747     268    1014

  Fragments arranged by size: 

                1014     962     764     747     432     268
 
AflIII A'CryG_T

Cuts at:   1113    1539    2643    3620    1113 
   Size:        426    1104     977    1680

  Fragments arranged by size: 

                1680    1104     977     426
 
AgeI A'CCGG_T

Cuts at:   2575    2575 
   Size:       4187
 
AhdI GACnn_n'nnGTC

Cuts at:    710     710 
   Size:       4187
 
AleI CACnn'nnGTG

Cuts at:    784     784 
   Size:       4187
 
AlwNI CAG_nnn'CTG

Cuts at:    237     525    1492    3059     237 
   Size:        288     967    1567    1365

  Fragments arranged by size: 

                1567    1365     967     288
 
ApaI G_GGCC'C

Cuts at:    209     209 
   Size:       4187
 
ApaLI G'TGCA_C

Cuts at:   2957    2957 
   Size:       4187
 
ApoI r'AATT_y

Cuts at:   1191    3540    3796    1191 
   Size:       2349     256    1582

  Fragments arranged by size: 

                2349    1582     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4187
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4187
 
AvaI C'yCGr_G

Cuts at:   1207    1207 
   Size:       4187
 
BamHI G'GATC_C

Cuts at:   1212    1212 
   Size:       4187
 
BanI G'GyrC_C

Cuts at:   1203    1617    1865    1900    1203 
   Size:        414     248      35    3490

  Fragments arranged by size: 

                3490     414     248      35
 
BanII G_rGCy'C

Cuts at:    209     968    1201    2231     209 
   Size:        759     233    1030    2165

  Fragments arranged by size: 

                2165    1030     759     233
 
BbeI G_GCGC'C

Cuts at:   1621    1869    1621 
   Size:        248    3939
 
BciVI GTATCCnnnnn_n'

Cuts at:   2085    2846    4187    2085 
   Size:        761    1341    2085

  Fragments arranged by size: 

                2085    1341     761
 
BclI T'GATC_A

Cuts at:   1707    1707 
   Size:       4187
 
BglI GCCn_nnn'nGGC

Cuts at:   1076    1076 
   Size:       4187
 
BglII A'GATC_T

Cuts at:    284    1702    3739     284 
   Size:       1418    2037     732

  Fragments arranged by size: 

                2037    1418     732
 
BlpI GC'TnA_GC

Cuts at:   1299    1299 
   Size:       4187
 
Bme1580I G_kGCm'C

Cuts at:    194     209    1812    1905    2961     194 
   Size:         15    1603      93    1056    1420

  Fragments arranged by size: 

                1603    1420    1056      93      15
 
BmrI ACTGGGnnnn_n'

Cuts at:    791    1399    1480    1815    3959     791 
   Size:        608      81     335    2144    1019

  Fragments arranged by size: 

                2144    1019     608     335      81
 
BmtI G_CTAG'C

Cuts at:    176     176 
   Size:       4187
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2554    2554 
   Size:       4187
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    413     562     568     738     413 
   Size:        149       6     170    3862

  Fragments arranged by size: 

                3862     170     149       6
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1363    2242    2734    3032    1363 
   Size:        879     492     298    2518

  Fragments arranged by size: 

                2518     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    665    3891     665 
   Size:       3226     961
 
BsaAI yAC'GTr

Cuts at:   1116    1540    1116 
   Size:        424    3763
 
BsaBI GATnn'nnATC

Cuts at:      6     565    1701    3738       6 
   Size:        559    1136    2037     455

  Fragments arranged by size: 

                2037    1136     559     455
 
BsaHI Gr'CG_yC

Cuts at:    401    1618    1866    2568    4064     401 
   Size:       1217     248     702    1496     524

  Fragments arranged by size: 

                1496    1217     702     524     248
 
BsaWI w'CCGG_w

Cuts at:   1376    1897    2575    2849    2996    3407    1376 
   Size:        521     678     274     147     411    2156

  Fragments arranged by size: 

                2156     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    336     366     386     391     416     421     613     643     336 
   Size:         30      20       5      25       5     192      30    3880

  Fragments arranged by size: 

                3880     192      30      30      25      20       5       5
            
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    399     468     946     399 
   Size:         69     478    3640

  Fragments arranged by size: 

                3640     478      69
 
BseYI C'CCAG_C

Cuts at:    230     373     378     624     637    1612    2370    2947    3443 
   Size:        143       5     246      13     975     758     577     496

Cuts at:   3443     230 
   Size:        974

  Fragments arranged by size: 

                 975     974     758     577     496     246     143      13
                   5
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1079    1079 
   Size:       4187
 
BsiEI CG_ry'CG

Cuts at:    104    1729    1775    2983     104 
   Size:       1625      46    1208    1308

  Fragments arranged by size: 

                1625    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:   1201    1979    2169    2961    1201 
   Size:        778     190     792    2427

  Fragments arranged by size: 

                2427     792     778     190
 
BsmBI CGTCTCn'nnnn_

Cuts at:    761    1002    1096     761 
   Size:        241      94    3852

  Fragments arranged by size: 

                3852     241      94
 
Bsp1286I G_dGCh'C

Cuts at:    194     209     968    1201    1812    1905    1979    2169    2231 
   Size:         15     759     233     611      93      74     190      62

Cuts at:   2231    2961     194 
   Size:        730    1420

  Fragments arranged by size: 

                1420     759     730     611     233     190      93      74
                  62      15
 
BspEI T'CCGG_A

Cuts at:   3407    3407 
   Size:       4187
 
BspHI T'CATG_A

Cuts at:   3363    3363 
   Size:       4187
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    441     545    1237    1451    1753    2134     441 
   Size:        104     692     214     302     381    2494

  Fragments arranged by size: 

                2494     692     381     302     214     104
 
BsrBI CCG'CTC

Cuts at:     50     300    2479    2533    4017    4182      50 
   Size:        250    2179      54    1484     165      55

  Fragments arranged by size: 

                2179    1484     250     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   2099    2099 
   Size:       4187
 
BsrFI r'CCGG_y

Cuts at:    221    2185    2366    2575     221 
   Size:       1964     181     209    1833

  Fragments arranged by size: 

                1964    1833     209     181
 
BsrGI T'GTAC_A

Cuts at:    693     693 
   Size:       4187
 
BssSI C'ACGA_G

Cuts at:   2458    2816    2458 
   Size:        358    3829
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1564    1564 
   Size:       4187
 
BstBI TT'CG_AA

Cuts at:   2548    2548 
   Size:       4187
 
BstXI CCAn_nnnn'nTGG

Cuts at:    573     573 
   Size:       4187
 
BstYI r'GATC_y

Cuts at:      1     284     857    1212    1679    1702    2037    2283    3295 
   Size:        283     573     355     467      23     335     246    1012

Cuts at:   3295    3381    3393    3739       1 
   Size:         86      12     346     449

  Fragments arranged by size: 

                1012     573     467     449     355     346     335     283
                 246      86      23      12
 
BtgI C'CryG_G

Cuts at:    107     107 
   Size:       4187
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    417    2137    2318    2481     417 
   Size:       1720     181     163    2123

  Fragments arranged by size: 

                2123    1720     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1430    1430 
   Size:       4187
 
ClaI AT'CG_AT

Cuts at:   1031    1031 
   Size:       4187
 
DraI TTT'AAA

Cuts at:   1186    1186 
   Size:       4187
 
DrdI GACnn_nn'nnGTC

Cuts at:   1893    2751    1893 
   Size:        858    3329
 
EaeI y'GGCC_r

Cuts at:    872    1118    1772    1946    2337    2364    3709     872 
   Size:        246     654     174     391      27    1345    1350

  Fragments arranged by size: 

                1350    1345     654     391     246     174      27
 
EagI C'GGCC_G

Cuts at:   1772    1772 
   Size:       4187
 
EarI CTCTTCn'nnn_

Cuts at:    262     307     478     844    2210    2420     262 
   Size:         45     171     366    1366     210    2029

  Fragments arranged by size: 

                2029    1366     366     210     171      45
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2395    2705    2851    3997    2395 
   Size:        310     146    1146    2585

  Fragments arranged by size: 

                2585    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   1199    1199 
   Size:       4187
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    286     413     562     568     738    1050    2012    2444    3191 
   Size:        127     149       6     170     312     962     432     747

Cuts at:   3191    3459     286 
   Size:        268    1014

  Fragments arranged by size: 

                1014     962     747     432     312     268     170     149
                 127       6
 
EcoO109I rG'GnC_Cy

Cuts at:    205     206     467     704     726    1326     205 
   Size:          1     261     237      22     600    3066

  Fragments arranged by size: 

                3066     600     261     237      22       1
 
EcoRI G'AATT_C

Cuts at:   1191    1191 
   Size:       4187
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2206    2206 
   Size:       4187
 
FspI TGC'GCA

Cuts at:   1968    1968 
   Size:       4187
 
HaeII r_GCGC'y

Cuts at:   1621    1869    2891    3870    1621 
   Size:        248    1022     979    1938

  Fragments arranged by size: 

                1938    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    284     316     386     391     418     423     673     696     705 
   Size:         32      70       5      27       5     250      23       9

Cuts at:    705     728    2029    2059    2061    2091     284 
   Size:         23    1301      30       2      30    2380

  Fragments arranged by size: 

                2380    1301     250      70      32      30      30      27
                  23      23       9       5       5       2
 
HincII GTy'rAC

Cuts at:    984    1226     984 
   Size:        242    3945
 
KasI G'GCGC_C

Cuts at:   1617    1865    1617 
   Size:        248    3939
 
KpnI G_GTAC'C

Cuts at:   1207    1207 
   Size:       4187
 
MluI A'CGCG_T

Cuts at:   3620    3620 
   Size:       4187
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    506    1064    2858    3042     506 
   Size:        558    1794     184    1651

  Fragments arranged by size: 

                1794    1651     558     184
 
MscI TGG'CCA

Cuts at:    874    1948     874 
   Size:       1074    3113
 
MslI CAynn'nnrTG

Cuts at:    520     784    2176    2303     520 
   Size:        264    1392     127    2404

  Fragments arranged by size: 

                2404    1392     264     127
 
MspA1I CmG'CkG

Cuts at:    948    1298    1612    1972    2985    3230     948 
   Size:        350     314     360    1013     245    1905

  Fragments arranged by size: 

                1905    1013     360     350     314     245
 
NaeI GCC'GGC

Cuts at:   2368    2368 
   Size:       4187
 
NarI GG'CG_CC

Cuts at:   1618    1866    1618 
   Size:        248    3939
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       4187
 
NgoMIV G'CCGG_C

Cuts at:   2366    2366 
   Size:       4187
 
NheI G'CTAG_C

Cuts at:    172     172 
   Size:       4187
 
NspI r_CATG'y

Cuts at:    350    1240    1740    2647     350 
   Size:        890     500     907    1890

  Fragments arranged by size: 

                1890     907     890     500
 
PciI A'CATG_T

Cuts at:   2643    2643 
   Size:       4187
 
PflMI CCAn_nnn'nTGG

Cuts at:    540     721     540 
   Size:        181    4006
 
PfoI T'CCnGG_A

Cuts at:   1131    1131 
   Size:       4187
 
PmeI GTTT'AAAC

Cuts at:   1186    1186 
   Size:       4187
 
PmlI CAC'GTG

Cuts at:   1116    1116 
   Size:       4187
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3326    3358    3326 
   Size:         32    4155
 
PpuMI rG'GwC_Cy

Cuts at:    704     704 
   Size:       4187
 
PspOMI G'GGCC_C

Cuts at:    205     205 
   Size:       4187
 
PstI C_TGCA'G

Cuts at:    604    1234    1919     604 
   Size:        630     685    2872

  Fragments arranged by size: 

                2872     685     630
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4187
 
PvuII CAG'CTG

Cuts at:    948    1612    1972     948 
   Size:        664     360    3163

  Fragments arranged by size: 

                3163     664     360
 
RsrII CG'GwC_CG

Cuts at:   2382    2382 
   Size:       4187
 
SacI G_AGCT'C

Cuts at:   1201    1201 
   Size:       4187
 
SalI G'TCGA_C

Cuts at:   1224    1224 
   Size:       4187
 
SanDI GG'GwC_CC

Cuts at:    704     704 
   Size:       4187
 
SapI GCTCTTCn'nnn_

Cuts at:    307    2210    2420     307 
   Size:       1903     210    2074

  Fragments arranged by size: 

                2074    1903     210
 
SbfI CC_TGCA'GG

Cuts at:   1234    1234 
   Size:       4187
 
ScaI AGT'ACT

Cuts at:   3704    3704 
   Size:       4187
 
SexAI A'CCwGG_T

Cuts at:    572     572 
   Size:       4187
 
SfcI C'TryA_G

Cuts at:     33     600    1230    1915    2908    3099      33 
   Size:        567     630     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     630     567     191
 
SfoI GGC'GCC

Cuts at:   1619    1867    1619 
   Size:        248    3939
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4187
 
SmaI CCC'GGG

Cuts at:   1209    1209 
   Size:       4187
 
SmlI C'TyrA_G

Cuts at:   1342    2257    2749    3011    1342 
   Size:        915     492     262    2518

  Fragments arranged by size: 

                2518     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4187
 
SphI G_CATG'C

Cuts at:   1240    1740    1240 
   Size:        500    3687
 
StyI C'CwwG_G

Cuts at:    107     762     866    1321    1675     107 
   Size:        655     104     455     354    2619

  Fragments arranged by size: 

                2619     655     455     354     104
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1770    1903    2362    2571    1770 
   Size:        133     459     209    3386

  Fragments arranged by size: 

                3386     459     209     133
 
TatI w'GTAC_w

Cuts at:    693    3702     693 
   Size:       3009    1178
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3704    3736    3704 
   Size:         32    4155
 
Tth111I GACn'n_nGTC

Cuts at:    574    1984     574 
   Size:       1410    2777
 
XbaI T'CTAG_A

Cuts at:   1218    1218 
   Size:       4187
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    489     682     726    1682     489 
   Size:        193      44     956    2994

  Fragments arranged by size: 

                2994     956     193      44
 
XmaI C'CCGG_G

Cuts at:   1207    1207 
   Size:       4187
 
ZraI GAC'GTC

Cuts at:    402     402 
   Size:       4187
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI   AflIII     AgeI
     AhdI     AleI    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbeI    BciVI     BclI     BglI
    BglII     BlpI Bme1580I     BmrI     BmtI     BplI     BpmI    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI    BstBI    BstXI
    BstYI     BtgI    BtgZI     BtsI     ClaI     DraI     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI
     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NheI
     NspI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
   PspOMI     PstI     PvuI    PvuII    RsrII     SacI     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI     SfoI     SgfI     SmaI
     SmlI     SpeI     SphI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XmaI     ZraI

 Enzymes that do not cut: 

     AfeI    AflII     AlfI     AloI     AscI    AvrII     BaeI     BbsI
    BbvCI     BcgI    BfrBI    BmgBI   Bpu10I    BsiWI     BsmI   BssHII
   BstEII  BstZ17I   Bsu36I    CspCI   DraIII    EcoNI    EcoRV     FseI
    FspAI  HindIII     HpaI     MfeI     NdeI     NotI     NruI     NsiI
     PacI     PasI    PshAI     PsiI    PspXI     PsrI    SacII     SfiI
    SgrAI    SnaBI     SrfI     SspI     StuI     SwaI     XhoI     XmnI
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