Restriction map for pF1KB8173
 (Linear) (Six-base) MAPSORT of: KIBB8173.seq  Check: 3533  from: 1  to: 1074



KIBB8173.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:03  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     437    1074 
   Size:        437     637
 
AatII G_ACGT'C

Cuts at:      0     296    1074 
   Size:        296     778
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     178     942    1074 
   Size:        178     764     132

  Fragments arranged by size: 

                 764     178     132
 
AflIII A'CryG_T

Cuts at:      0    1005    1074 
   Size:       1005      69
 
AhdI GACnn_n'nnGTC

Cuts at:      0     602    1074 
   Size:        602     472
 
AleI CACnn'nnGTG

Cuts at:      0     676    1074 
   Size:        676     398
 
AlwNI CAG_nnn'CTG

Cuts at:      0     129     417    1074 
   Size:        129     288     657

  Fragments arranged by size: 

                 657     288     129
 
ApaI G_GGCC'C

Cuts at:      0     101    1074 
   Size:        101     973
 
BanII G_rGCy'C

Cuts at:      0     101     860    1074 
   Size:        101     759     214

  Fragments arranged by size: 

                 759     214     101
 
BglI GCCn_nnn'nGGC

Cuts at:      0     968    1074 
   Size:        968     106
 
BglII A'GATC_T

Cuts at:      0     176    1074 
   Size:        176     898
 
Bme1580I G_kGCm'C

Cuts at:      0      86     101    1074 
   Size:         86      15     973

  Fragments arranged by size: 

                 973      86      15
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     683    1074 
   Size:        683     391
 
BmtI G_CTAG'C

Cuts at:      0      68    1074 
   Size:         68    1006
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     305     454     460     630    1074 
   Size:        305     149       6     170     444

  Fragments arranged by size: 

                 444     305     170     149       6
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     557    1074 
   Size:        557     517
 
BsaAI yAC'GTr

Cuts at:      0    1008    1074 
   Size:       1008      66
 
BsaBI GATnn'nnATC

Cuts at:      0     457    1074 
   Size:        457     617
 
BsaHI Gr'CG_yC

Cuts at:      0     293    1074 
   Size:        293     781
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     228     258     278     283     308     313     505     535 
   Size:        228      30      20       5      25       5     192      30

Cuts at:    535    1074 
   Size:        539

  Fragments arranged by size: 

                 539     228     192      30      30      25      20       5
                   5
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     291     360     838    1074 
   Size:        291      69     478     236

  Fragments arranged by size: 

                 478     291     236      69
 
BseYI C'CCAG_C

Cuts at:      0     122     265     270     516     529    1074 
   Size:        122     143       5     246      13     545

  Fragments arranged by size: 

                 545     246     143     122      13       5
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     971    1074 
   Size:        971     103
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     653     894     988    1074 
   Size:        653     241      94      86

  Fragments arranged by size: 

                 653     241      94      86
 
Bsp1286I G_dGCh'C

Cuts at:      0      86     101     860    1074 
   Size:         86      15     759     214

  Fragments arranged by size: 

                 759     214      86      15
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     333     437    1074 
   Size:        333     104     637

  Fragments arranged by size: 

                 637     333     104
 
BsrBI CCG'CTC

Cuts at:      0     192    1074 
   Size:        192     882
 
BsrFI r'CCGG_y

Cuts at:      0     113    1074 
   Size:        113     961
 
BsrGI T'GTAC_A

Cuts at:      0     585    1074 
   Size:        585     489
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     465    1074 
   Size:        465     609
 
BstYI r'GATC_y

Cuts at:      0     176     749    1074 
   Size:        176     573     325

  Fragments arranged by size: 

                 573     325     176
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     309    1074 
   Size:        309     765
 
ClaI AT'CG_AT

Cuts at:      0     923    1074 
   Size:        923     151
 
EaeI y'GGCC_r

Cuts at:      0     764    1010    1074 
   Size:        764     246      64

  Fragments arranged by size: 

                 764     246      64
 
EarI CTCTTCn'nnn_

Cuts at:      0     154     199     370     736    1074 
   Size:        154      45     171     366     338

  Fragments arranged by size: 

                 366     338     171     154      45
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     178     305     454     460     630     942    1074 
   Size:        178     127     149       6     170     312     132

  Fragments arranged by size: 

                 312     178     170     149     132     127       6
 
EcoO109I rG'GnC_Cy

Cuts at:      0      97      98     359     596     618    1074 
   Size:         97       1     261     237      22     456

  Fragments arranged by size: 

                 456     261     237      97      22       1
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     176     208     278     283     310     315     565     588 
   Size:        176      32      70       5      27       5     250      23

Cuts at:    588     597     620    1074 
   Size:          9      23     454

  Fragments arranged by size: 

                 454     250     176      70      32      27      23      23
                   9       5       5
 
HincII GTy'rAC

Cuts at:      0     876    1074 
   Size:        876     198
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     398     956    1074 
   Size:        398     558     118

  Fragments arranged by size: 

                 558     398     118
 
MscI TGG'CCA

Cuts at:      0     766    1074 
   Size:        766     308
 
MslI CAynn'nnrTG

Cuts at:      0     412     676    1074 
   Size:        412     264     398

  Fragments arranged by size: 

                 412     398     264
 
MspA1I CmG'CkG

Cuts at:      0     840    1074 
   Size:        840     234
 
NheI G'CTAG_C

Cuts at:      0      64    1074 
   Size:         64    1010
 
NspI r_CATG'y

Cuts at:      0     242    1074 
   Size:        242     832
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     432     613    1074 
   Size:        432     181     461

  Fragments arranged by size: 

                 461     432     181
 
PfoI T'CCnGG_A

Cuts at:      0    1023    1074 
   Size:       1023      51
 
PmlI CAC'GTG

Cuts at:      0    1008    1074 
   Size:       1008      66
 
PpuMI rG'GwC_Cy

Cuts at:      0     596    1074 
   Size:        596     478
 
PspOMI G'GGCC_C

Cuts at:      0      97    1074 
   Size:         97     977
 
PstI C_TGCA'G

Cuts at:      0     496    1074 
   Size:        496     578
 
PvuII CAG'CTG

Cuts at:      0     840    1074 
   Size:        840     234
 
SanDI GG'GwC_CC

Cuts at:      0     596    1074 
   Size:        596     478
 
SapI GCTCTTCn'nnn_

Cuts at:      0     199    1074 
   Size:        199     875
 
SexAI A'CCwGG_T

Cuts at:      0     464    1074 
   Size:        464     610
 
SfcI C'TryA_G

Cuts at:      0     492    1074 
   Size:        492     582
 
StyI C'CwwG_G

Cuts at:      0     654     758    1074 
   Size:        654     104     316

  Fragments arranged by size: 

                 654     316     104
 
TatI w'GTAC_w

Cuts at:      0     585    1074 
   Size:        585     489
 
Tth111I GACn'n_nGTC

Cuts at:      0     466    1074 
   Size:        466     608
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     381     574     618    1074 
   Size:        381     193      44     456

  Fragments arranged by size: 

                 456     381     193      44
 
ZraI GAC'GTC

Cuts at:      0     294    1074 
   Size:        294     780
 

 Enzymes that do cut:

     AarI    AatII     AcuI   AflIII     AhdI     AleI    AlwNI     ApaI
    BanII     BglI    BglII Bme1580I     BmrI     BmtI     BpmI     BsaI
    BsaAI    BsaBI    BsaHI    BsaXI    BseRI    BseYI     BsgI    BsmBI
 Bsp1286I    BspMI    BsrBI    BsrFI    BsrGI    BstXI    BstYI    BtgZI
     ClaI     EaeI     EarI  Eco57MI EcoO109I    Hin4I   HincII     MmeI
     MscI     MslI   MspA1I     NheI     NspI    PflMI     PfoI     PmlI
    PpuMI   PspOMI     PstI    PvuII    SanDI     SapI    SexAI     SfcI
     StyI     TatI  Tth111I     XcmI     ZraI

 Enzymes that do not cut: 

     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AlfI     AloI
    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
    BamHI     BanI     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BlpI    BmgBI     BplI   Bpu10I    BpuEI    BsaWI    BsiEI
  BsiHKAI    BsiWI     BsmI    BspEI    BspHI    BsrDI   BssHII    BssSI
   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I     BtgI     BtsI    CspCI
     DraI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NotI     NruI     NsiI     PacI     PasI     PciI     PmeI
     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII     SacI
    SacII     SalI     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI    TaqII     TstI     XbaI     XhoI     XmaI     XmnI
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