Restriction map for pF1KB8162
 (Linear) (Six-base) MAPSORT of: KIBB8162.seq  Check: 6590  from: 1  to: 1077



KIBB8162.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:03  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     836    1077 
   Size:        836     241
 
AflIII A'CryG_T

Cuts at:      0     240    1077 
   Size:        240     837
 
AleI CACnn'nnGTG

Cuts at:      0     772    1077 
   Size:        772     305
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     436    1077 
   Size:        436     641
 
ApaLI G'TGCA_C

Cuts at:      0     118    1077 
   Size:        118     959
 
ApoI r'AATT_y

Cuts at:      0    1008    1077 
   Size:       1008      69
 
AvaI C'yCGr_G

Cuts at:      0      29    1077 
   Size:         29    1048
 
BanII G_rGCy'C

Cuts at:      0     913    1062    1077 
   Size:        913     149      15

  Fragments arranged by size: 

                 913     149      15
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     134    1077 
   Size:        134     943
 
BbvCI CC'TCA_GC

Cuts at:      0     143    1077 
   Size:        143     934
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      34      68    1077 
   Size:         34      34    1009

  Fragments arranged by size: 

                1009      34      34
 
BfrBI ATG'CAT

Cuts at:      0     423     640    1077 
   Size:        423     217     437

  Fragments arranged by size: 

                 437     423     217
 
Bme1580I G_kGCm'C

Cuts at:      0     122     509     526    1077 
   Size:        122     387      17     551

  Fragments arranged by size: 

                 551     387     122      17
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     520    1077 
   Size:        520     557
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      72    1077 
   Size:         72    1005
 
Bpu10I CC'TnA_GC

Cuts at:      0     143    1077 
   Size:        143     934
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     740    1077 
   Size:        740     337
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     464    1077 
   Size:        464     613
 
BsaAI yAC'GTr

Cuts at:      0     243    1077 
   Size:        243     834
 
BsaWI w'CCGG_w

Cuts at:      0     306    1077 
   Size:        306     771
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     310    1077 
   Size:        310     767
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     148     448    1077 
   Size:        148     300     629

  Fragments arranged by size: 

                 629     300     148
 
BsiHKAI G_wGCw'C

Cuts at:      0     122     651    1077 
   Size:        122     529     426

  Fragments arranged by size: 

                 529     426     122
 
BsiWI C'GTAC_G

Cuts at:      0     535    1077 
   Size:        535     542
 
BsmI GAATG_Cn'

Cuts at:      0     425    1077 
   Size:        425     652
 
Bsp1286I G_dGCh'C

Cuts at:      0     122     509     526     651     913    1062    1077 
   Size:        122     387      17     125     262     149      15

  Fragments arranged by size: 

                 387     262     149     125     122      17      15
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     170    1077 
   Size:        170     907
 
BsrBI CCG'CTC

Cuts at:      0     357     680    1077 
   Size:        357     323     397

  Fragments arranged by size: 

                 397     357     323
 
BsrFI r'CCGG_y

Cuts at:      0     443    1077 
   Size:        443     634
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     367    1077 
   Size:        367     710
 
BstBI TT'CG_AA

Cuts at:      0     591    1077 
   Size:        591     486
 
BtgI C'CryG_G

Cuts at:      0     605    1077 
   Size:        605     472
 
BtsI GCAGTG_nn'

Cuts at:      0     377     913    1077 
   Size:        377     536     164

  Fragments arranged by size: 

                 536     377     164
 
EarI CTCTTCn'nnn_

Cuts at:      0      67    1077 
   Size:         67    1010
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     836    1077 
   Size:        836     241
 
EcoO109I rG'GnC_Cy

Cuts at:      0     387    1077 
   Size:        387     690
 
EcoRI G'AATT_C

Cuts at:      0    1008    1077 
   Size:       1008      69
 
HaeII r_GCGC'y

Cuts at:      0     842    1077 
   Size:        842     235
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     659     691    1077 
   Size:        659      32     386

  Fragments arranged by size: 

                 659     386      32
 
HindIII A'AGCT_T

Cuts at:      0     175     224    1077 
   Size:        175      49     853

  Fragments arranged by size: 

                 853     175      49
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     927    1077 
   Size:        927     150
 
MslI CAynn'nnrTG

Cuts at:      0     196     772    1077 
   Size:        196     576     305

  Fragments arranged by size: 

                 576     305     196
 
MspA1I CmG'CkG

Cuts at:      0     142     700     709     856    1077 
   Size:        142     558       9     147     221

  Fragments arranged by size: 

                 558     221     147     142       9
 
NaeI GCC'GGC

Cuts at:      0     445    1077 
   Size:        445     632
 
NcoI C'CATG_G

Cuts at:      0     605    1077 
   Size:        605     472
 
NdeI CA'TA_TG

Cuts at:      0     642    1077 
   Size:        642     435
 
NgoMIV G'CCGG_C

Cuts at:      0     443    1077 
   Size:        443     634
 
NsiI A_TGCA'T

Cuts at:      0     425     642    1077 
   Size:        425     217     435

  Fragments arranged by size: 

                 435     425     217
 
NspI r_CATG'y

Cuts at:      0     427    1077 
   Size:        427     650
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     835    1077 
   Size:        835     242
 
PmlI CAC'GTG

Cuts at:      0     243    1077 
   Size:        243     834
 
PstI C_TGCA'G

Cuts at:      0     876     930    1077 
   Size:        876      54     147

  Fragments arranged by size: 

                 876     147      54
 
PvuII CAG'CTG

Cuts at:      0     142     856    1077 
   Size:        142     714     221

  Fragments arranged by size: 

                 714     221     142
 
SfcI C'TryA_G

Cuts at:      0      38     872     926    1077 
   Size:         38     834      54     151

  Fragments arranged by size: 

                 834     151      54      38
 
SmaI CCC'GGG

Cuts at:      0      31    1077 
   Size:         31    1046
 
SmlI C'TyrA_G

Cuts at:      0     755    1077 
   Size:        755     322
 
StyI C'CwwG_G

Cuts at:      0     605    1077 
   Size:        605     472
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1070    1077 
   Size:       1070       7
 
Tth111I GACn'n_nGTC

Cuts at:      0     741    1077 
   Size:        741     336
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     240    1077 
   Size:        240     837
 
XmaI C'CCGG_G

Cuts at:      0      29    1077 
   Size:         29    1048
 
XmnI GAAnn'nnTTC

Cuts at:      0      10    1077 
   Size:         10    1067
 

 Enzymes that do cut:

     AcuI   AflIII     AleI     AlfI    ApaLI     ApoI     AvaI    BanII
     BbsI    BbvCI     BcgI    BfrBI Bme1580I     BmrI     BplI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaWI    BseRI     BsgI  BsiHKAI    BsiWI
     BsmI Bsp1286I    BspMI    BsrBI    BsrFI   BstAPI    BstBI     BtgI
     BtsI     EarI  Eco57MI EcoO109I    EcoRI    HaeII    Hin4I  HindIII
     MmeI     MslI   MspA1I     NaeI     NcoI     NdeI   NgoMIV     NsiI
     NspI    PflMI     PmlI     PstI    PvuII     SfcI     SmaI     SmlI
     StyI    TaqII  Tth111I     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AloI    AlwNI     ApaI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BanI     BbeI    BciVI     BclI     BglI    BglII
     BlpI    BmgBI     BmtI     BpmI    BsaBI    BsaHI    BsaXI    BseYI
    BsiEI    BsmBI    BspEI    BspHI    BsrDI    BsrGI   BssHII    BssSI
   BstEII    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI
    EcoRV     FalI     FseI     FspI    FspAI   HincII     HpaI     KasI
     KpnI     MfeI     MluI     MscI     NarI     NheI     NotI     NruI
     PacI     PasI     PciI     PfoI     PmeI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI     TatI     TstI     XbaI     XhoI     ZraI
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