Restriction map for pF1KB8156
 (Linear) (Six-base) MAPSORT of: KIBB8156.seq  Check: 5373  from: 1  to: 1212



KIBB8156.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:03  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     282    1212 
   Size:        282     930
 
AatII G_ACGT'C

Cuts at:      0    1007    1212 
   Size:       1007     205
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      76     424    1212 
   Size:         76     348     788

  Fragments arranged by size: 

                 788     348      76
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1156    1188    1212 
   Size:       1156      32      24

  Fragments arranged by size: 

                1156      32      24
 
AlwNI CAG_nnn'CTG

Cuts at:      0     324    1212 
   Size:        324     888
 
ApaLI G'TGCA_C

Cuts at:      0     145    1212 
   Size:        145    1067
 
ApoI r'AATT_y

Cuts at:      0     136     358    1045    1086    1212 
   Size:        136     222     687      41     126

  Fragments arranged by size: 

                 687     222     136     126      41
 
AvaI C'yCGr_G

Cuts at:      0      13     305    1212 
   Size:         13     292     907

  Fragments arranged by size: 

                 907     292      13
 
BanII G_rGCy'C

Cuts at:      0     181     776     881    1212 
   Size:        181     595     105     331

  Fragments arranged by size: 

                 595     331     181     105
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     215     418    1212 
   Size:        215     203     794

  Fragments arranged by size: 

                 794     215     203
 
BbvCI CC'TCA_GC

Cuts at:      0    1016    1212 
   Size:       1016     196
 
BclI T'GATC_A

Cuts at:      0     504    1212 
   Size:        504     708
 
BglII A'GATC_T

Cuts at:      0      55     703    1212 
   Size:         55     648     509

  Fragments arranged by size: 

                 648     509      55
 
BlpI GC'TnA_GC

Cuts at:      0     809    1212 
   Size:        809     403
 
Bme1580I G_kGCm'C

Cuts at:      0     149    1212 
   Size:        149    1063
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     334    1212 
   Size:        334     878
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     620    1212 
   Size:        620     592
 
Bpu10I CC'TnA_GC

Cuts at:      0     914    1016    1212 
   Size:        914     102     196

  Fragments arranged by size: 

                 914     196     102
 
BsaBI GATnn'nnATC

Cuts at:      0      60     509    1212 
   Size:         60     449     703

  Fragments arranged by size: 

                 703     449      60
 
BsaHI Gr'CG_yC

Cuts at:      0    1004    1212 
   Size:       1004     208
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1156    1186    1212 
   Size:       1156      30      26

  Fragments arranged by size: 

                1156      30      26
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      49     871    1212 
   Size:         49     822     341

  Fragments arranged by size: 

                 822     341      49
 
BseYI C'CCAG_C

Cuts at:      0     256    1212 
   Size:        256     956
 
BsiHKAI G_wGCw'C

Cuts at:      0     149     881    1212 
   Size:        149     732     331

  Fragments arranged by size: 

                 732     331     149
 
Bsp1286I G_dGCh'C

Cuts at:      0     149     181     776     881    1212 
   Size:        149      32     595     105     331

  Fragments arranged by size: 

                 595     331     149     105      32
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     282    1212 
   Size:        282     930
 
BstYI r'GATC_y

Cuts at:      0      55     510     703     918    1212 
   Size:         55     455     193     215     294

  Fragments arranged by size: 

                 455     294     215     193      55
 
BtgI C'CryG_G

Cuts at:      0       5    1212 
   Size:          5    1207
 
BtsI GCAGTG_nn'

Cuts at:      0     160    1212 
   Size:        160    1052
 
EaeI y'GGCC_r

Cuts at:      0       2    1212 
   Size:          2    1210
 
EarI CTCTTCn'nnn_

Cuts at:      0     764    1093    1212 
   Size:        764     329     119

  Fragments arranged by size: 

                 764     329     119
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     540    1212 
   Size:        540     672
 
EcoICRI GAG'CTC

Cuts at:      0     879    1212 
   Size:        879     333
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      76     424     620    1212 
   Size:         76     348     196     592

  Fragments arranged by size: 

                 592     348     196      76
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     799    1212 
   Size:        799     413
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     309     454     939    1212 
   Size:        309     145     485     273

  Fragments arranged by size: 

                 485     309     273     145
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     116     148     690     722    1212 
   Size:        116      32     542      32     490

  Fragments arranged by size: 

                 542     490     116      32      32
 
MslI CAynn'nnrTG

Cuts at:      0     713    1212 
   Size:        713     499
 
MspA1I CmG'CkG

Cuts at:      0       7     444    1212 
   Size:          7     437     768

  Fragments arranged by size: 

                 768     437       7
 
NspI r_CATG'y

Cuts at:      0     897    1159    1212 
   Size:        897     262      53

  Fragments arranged by size: 

                 897     262      53
 
PfoI T'CCnGG_A

Cuts at:      0     494    1212 
   Size:        494     718
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1156    1188    1212 
   Size:       1156      32      24

  Fragments arranged by size: 

                1156      32      24
 
PstI C_TGCA'G

Cuts at:      0      26    1212 
   Size:         26    1186
 
SacI G_AGCT'C

Cuts at:      0     881    1212 
   Size:        881     331
 
SacII CC_GC'GG

Cuts at:      0       8    1212 
   Size:          8    1204
 
SapI GCTCTTCn'nnn_

Cuts at:      0     764    1212 
   Size:        764     448
 
SfcI C'TryA_G

Cuts at:      0      22    1212 
   Size:         22    1190
 
SmlI C'TyrA_G

Cuts at:      0     305    1212 
   Size:        305     907
 
Tth111I GACn'n_nGTC

Cuts at:      0     130     417    1212 
   Size:        130     287     795

  Fragments arranged by size: 

                 795     287     130
 
XhoI C'TCGA_G

Cuts at:      0     305    1212 
   Size:        305     907
 
XmnI GAAnn'nnTTC

Cuts at:      0     434     640    1212 
   Size:        434     206     572

  Fragments arranged by size: 

                 572     434     206
 
ZraI GAC'GTC

Cuts at:      0    1005    1212 
   Size:       1005     207
 

 Enzymes that do cut:

     AarI    AatII     AcuI     AloI    AlwNI    ApaLI     ApoI     AvaI
    BanII     BbsI    BbvCI     BclI    BglII     BlpI Bme1580I     BmrI
     BpmI   Bpu10I    BsaBI    BsaHI    BsaXI    BseRI    BseYI  BsiHKAI
 Bsp1286I    BspMI    BstYI     BtgI     BtsI     EaeI     EarI     EciI
  EcoICRI  Eco57MI    EcoNI     FalI    Hin4I     MslI   MspA1I     NspI
     PfoI     PpiI     PstI     SacI    SacII     SapI     SfcI     SmlI
  Tth111I     XhoI     XmnI     ZraI

 Enzymes that do not cut: 

     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     ApaI     AscI     AseI    AsiSI    AvrII     BaeI
    BamHI     BanI     BbeI     BcgI    BciVI    BfrBI     BglI    BmgBI
     BmtI     BplI    BpuEI     BsaI    BsaAI    BsaWI     BsgI    BsiEI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EagI
 EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI     PciI    PflMI     PmeI     PmlI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII
     SalI    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     StyI     SwaI    TaqII     TatI     TstI     XbaI     XcmI     XmaI
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