Restriction map for pF1KB8147
 (Linear) (Six-base) MAPSORT of: KIBB8147.seq  Check: 1803  from: 1  to: 804



KIBB8147.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:03  ..

AccI GT'mk_AC

Cuts at:      0      14      48     728     804 
   Size:         14      34     680      76

  Fragments arranged by size: 

                 680      76      34      14
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     136     168     804 
   Size:        136      32     636

  Fragments arranged by size: 

                 636     136      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     124     804 
   Size:        124     680
 
ApaI G_GGCC'C

Cuts at:      0     781     804 
   Size:        781      23
 
ApoI r'AATT_y

Cuts at:      0     184     316     804 
   Size:        184     132     488

  Fragments arranged by size: 

                 488     184     132
 
BanII G_rGCy'C

Cuts at:      0     781     804 
   Size:        781      23
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     629     804 
   Size:        629     175
 
BlpI GC'TnA_GC

Cuts at:      0     194     804 
   Size:        194     610
 
Bme1580I G_kGCm'C

Cuts at:      0     781     804 
   Size:        781      23
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     413     804 
   Size:        413     391
 
Bpu10I CC'TnA_GC

Cuts at:      0     117     804 
   Size:        117     687
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     802     804 
   Size:        802       2
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     491     804 
   Size:        491     313
 
BsiHKAI G_wGCw'C

Cuts at:      0     118     804 
   Size:        118     686
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     560     804 
   Size:        560     244
 
Bsp1286I G_dGCh'C

Cuts at:      0     118     781     804 
   Size:        118     663      23

  Fragments arranged by size: 

                 663     118      23
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     229     585     804 
   Size:        229     356     219

  Fragments arranged by size: 

                 356     229     219
 
BsrDI GCAATG_nn'

Cuts at:      0     164     804 
   Size:        164     640
 
BssSI C'ACGA_G

Cuts at:      0     205     804 
   Size:        205     599
 
BstZ17I GTA'TAC

Cuts at:      0      15     804 
   Size:         15     789
 
Bsu36I CC'TnA_GG

Cuts at:      0     518     804 
   Size:        518     286
 
BtsI GCAGTG_nn'

Cuts at:      0      11     413     474     804 
   Size:         11     402      61     330

  Fragments arranged by size: 

                 402     330      61      11
 
EarI CTCTTCn'nnn_

Cuts at:      0     791     804 
   Size:        791      13
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     413     804 
   Size:        413     391
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     770     804 
   Size:        770      34
 
EcoO109I rG'GnC_Cy

Cuts at:      0     777     804 
   Size:        777      27
 
EcoRI G'AATT_C

Cuts at:      0     316     804 
   Size:        316     488
 
HincII GTy'rAC

Cuts at:      0      49     261     600     804 
   Size:         49     212     339     204

  Fragments arranged by size: 

                 339     212     204      49
 
MspA1I CmG'CkG

Cuts at:      0     508     580     804 
   Size:        508      72     224

  Fragments arranged by size: 

                 508     224      72
 
NspI r_CATG'y

Cuts at:      0      60     150     804 
   Size:         60      90     654

  Fragments arranged by size: 

                 654      90      60
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0       1      33     804 
   Size:          1      32     771

  Fragments arranged by size: 

                 771      32       1
 
PspOMI G'GGCC_C

Cuts at:      0     777     804 
   Size:        777      27
 
PstI C_TGCA'G

Cuts at:      0      86     408     804 
   Size:         86     322     396

  Fragments arranged by size: 

                 396     322      86
 
PvuII CAG'CTG

Cuts at:      0     580     804 
   Size:        580     224
 
SalI G'TCGA_C

Cuts at:      0      47     804 
   Size:         47     757
 
SfcI C'TryA_G

Cuts at:      0      82     404     804 
   Size:         82     322     400

  Fragments arranged by size: 

                 400     322      82
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     244     804 
   Size:        244     560
 
TatI w'GTAC_w

Cuts at:      0     209     804 
   Size:        209     595
 
Tth111I GACn'n_nGTC

Cuts at:      0     392     804 
   Size:        392     412
 
XmnI GAAnn'nnTTC

Cuts at:      0     320     340     804 
   Size:        320      20     464

  Fragments arranged by size: 

                 464     320      20
 

 Enzymes that do cut:

     AccI     AloI    AlwNI     ApaI     ApoI    BanII     BbsI     BlpI
 Bme1580I     BpmI   Bpu10I    BseRI     BsgI  BsiHKAI    BsmBI Bsp1286I
    BspMI    BsrDI    BssSI  BstZ17I   Bsu36I     BtsI     EarI  Eco57MI
    EcoNI EcoO109I    EcoRI   HincII   MspA1I     NspI     PpiI   PspOMI
     PstI    PvuII     SalI     SfcI    TaqII     TatI  Tth111I     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII    BmgBI     BmrI     BmtI
     BplI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseYI    BsiEI    BsiWI     BsmI    BspEI    BspHI    BsrBI    BsrFI
    BsrGI   BssHII   BstAPI    BstBI   BstEII    BstXI    BstYI     BtgI
    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EciI  EcoICRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
    Hin4I  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI    PpuMI    PshAI     PsiI    PspXI     PsrI     PvuI
    RsrII     SacI    SacII    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     StyI     SwaI     TstI     XbaI
     XcmI     XhoI     XmaI     ZraI
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