Restriction map for pF1KB8136
##################################
# NO CUT ENZYMES (  111 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AflII       AgeI        AhdI        AscI        
AseI        AsnI        Asp718I     AspEI       AviII       
AvrII       BamHI       BanI        BbeI        BcgI        
BciVI       BclI        BfrI        BglI        BglII       
BlnI        BsaBI       BsaHI       BsaWI       BseAI       
BsiEI       BsmI        Bsp1407I    BspDI       BspEI       
BsrDI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstBI       BstEII      BstPI       BstYI       
BstZ17I     Cfr10I      ClaI        CpoI        DraI        
EagI        Eam1105I    EclXI       Eco52I      EcoO65I     
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
FspI        Hin1I       HincII      HindII      HindIII     
HpaI        KasI        KpnI        KspI        MamI        
MfeI        MflI        MroI        MunI        NaeI        
NarI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PinAI       PmeI        PshAI       PshBI       Psp1406I    
PvuI        RsrII       SacI        SacII       SalI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmlI        SnaBI       SpeI        SphI        SspBI       
SspI        StuI        SwaI        XcmI        XhoI        
XhoII       


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AlwI          446  GGATC                GGATC(4/5)
AocI          963  CCTGAGG              CC^T[ACGT]AGG
Aor51HI       820  AGCGCT               AGC^GCT
ApaI         1084  GGGCCC               GGGCC^C
Asp700I       163  GAAAAAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI          107  TGGCCA               TGG^CCA
BanII        1084  GGGCCC               G[GA]GC[CT]^C
BbvCI         816  CCTCAGC              CCTCAGC(-5/-2)
BlpI         1074  GCTCAGC              GC^T[ACGT]AGC
BpmI         1293  CTGGAG               CTGGAG(16/14)
Bpu1102I     1074  GCTCAGC              GC^T[ACGT]AGC
BsiWI         803  CGTACG               C^GTACG
BsmBI        1157  GAGACG               CGTCTC(1/5)
BspLU11I     1211  ACATGT               A^CATGT
BsrBI         485  GAGCGG               CCGCTC(-3/-3)
BstXI         506  CCAGTGAGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        963  CCTGAGG              CC^T[ACGT]AGG
CelII        1074  GCTCAGC              GC^T[ACGT]AGC
DraIII        742  CACCTTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         1132  GACGACCCAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI          728  CCATGG               C^C[GA][CT]GG
EaeI          107  TGGCCA               [CT]^GGCC[GA]
Eco47III      820  AGCGCT               AGC^GCT
Eco81I        963  CCTGAGG              CC^T[ACGT]AGG
EcoT14I       728  CCATGG               C^C[AT][AT]GG
HaeII         820  AGCGCT               [GA]GCGC^[CT]
MluI          800  ACGCGT               A^CGCGT
MluNI         107  TGGCCA               TGG^CCA
MscI          107  TGGCCA               TGG^CCA
MseI          842  TTAA                 T^TAA
MspA1I        532  CAGCTG               C[AC]G^C[GT]G
NcoI          728  CCATGG               C^CATGG
NspI         1211  ACATGT               [GA]CATG^[CT]
PflMI        1100  CCACACACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          670  GAGTC                GAGTC(4/5)
PvuII         532  CAGCTG               CAG^CTG
ScaI          302  AGTACT               AGT^ACT
SfaNI         691  GATGC                GCATC(5/9)
SmaI          686  CCCGGG               CCC^GGG
StyI          728  CCATGG               C^C[AT][AT]GG
Tru9I         842  TTAA                 T^TAA
Van91I       1100  CCACACACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI          954  TCTAGA               T^CTAGA
XmaCI         686  CCCGGG               C^CCGGG
XmaI          686  CCCGGG               C^CCGGG
XmnI          163  GAAAAAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AcsI          167  AAATTC               [GA]^AATT[CT]
              944  AAATTT              
Alw44I        464  GTGCAC               G^TGCAC
              909  GTGCAC              
ApaLI         464  GTGCAC               G^TGCAC
              909  GTGCAC              
ApoI          167  AAATTC               [GA]^AATT[CT]
              944  AAATTT              
BbrPI         259  CACGTG               CAC^GTG
              285  CACGTG              
BbsI          889  GAAGAC               GAAGAC(2/6)
             1282  GTCTTC              
BfaI          482  CTAG                 C^TAG
              955  CTAG                
BpuAI         889  GAAGAC               GAAGAC(2/6)
             1282  GTCTTC              
BsaAI         259  CACGTG               [CT]AC^GT[GA]
              285  CACGTG              
BseRI          97  GAGGAG               GAGGAG(10/8)
              158  GAGGAG              
BsgI         1006  CTGCAC               GTGCAG(16/14)
               57  GTGCAG              
BspHI         662  TCATGA               T^CATGA
             1124  TCATGA              
BspMI         643  ACCTGC               ACCTGC(4/8)
             1068  GCAGGT              
BstAPI        466  GCACACTATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              741  GCACCTTGTGC         
EcoNI          75  CCTGCAGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1047  CCTGCAGGAGG         
HgaI         1116  GACGC                GACGC(5/10)
             1031  GCGTC               
MaeI          482  CTAG                 C^TAG
              955  CTAG                
PmaCI         259  CACGTG               CAC^GTG
              285  CACGTG              
PmlI          259  CACGTG               CAC^GTG
              285  CACGTG              
PpuMI         640  GGGACCT              [GA]G^G[AT]CC[CT]
             1000  GGGACCC             
RcaI          662  TCATGA               T^CATGA
             1124  TCATGA              
SapI           43  GAAGAGC              GCTCTTC(1/4)
              786  GCTCTTC             
SbfI           75  CCTGCAGG             CCTGCA^GG
             1047  CCTGCAGG            
Sse8387I       75  CCTGCAGG             CCTGCA^GG
             1047  CCTGCAGG            
TaqI          668  TCGA                 T^CGA
              983  TCGA                
TfiI           31  GAATC                G^A[AT]TC
              400  GAATC               
Tsp509I       168  AATT                 ^AATT
              945  AATT                
TthHB8I       668  TCGA                 T^CGA
              983  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   73 enzymes) #
#########################################

AccII       AciI        AfaI        AflIII      AluI        
AlwNI       AspHI       AspI        AvaI        AvaII       
BbvI        BcnI        BmyI        BsaI        BsaJI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       BstUI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraII       EarI        EcoO109I    
EcoRII      FauI        Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeII       MaeIII      
MboI        MboII       MnlI        MslI        MspI        
MvaI        MvnI        MwoI        NciI        NdeII       
NlaIII      NlaIV       PflFI       PstI        RsaI        
Sau3AI      Sau96I      ScrFI       SfcI        TseI        
Tsp45I      TspRI       Tth111I     
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