Restriction map for pF1KB8136
 (Linear) (Six-base) MAPSORT of: KIBB8136.seq  Check: 1693  from: 1  to: 1299



KIBB8136.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:03  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1059    1299 
   Size:       1059     240
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     721    1299 
   Size:        721     578
 
AfeI AGC'GCT

Cuts at:      0     822    1299 
   Size:        822     477
 
AflIII A'CryG_T

Cuts at:      0     284     800    1211    1299 
   Size:        284     516     411      88

  Fragments arranged by size: 

                 516     411     284      88
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     934    1299 
   Size:        934     365
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1047    1119    1144    1299 
   Size:       1047      72      25     155

  Fragments arranged by size: 

                1047     155      72      25
 
ApaI G_GGCC'C

Cuts at:      0    1088    1299 
   Size:       1088     211
 
ApaLI G'TGCA_C

Cuts at:      0     464     909    1299 
   Size:        464     445     390

  Fragments arranged by size: 

                 464     445     390
 
ApoI r'AATT_y

Cuts at:      0     167     944    1299 
   Size:        167     777     355

  Fragments arranged by size: 

                 777     355     167
 
AvaI C'yCGr_G

Cuts at:      0     277     604     686    1299 
   Size:        277     327      82     613

  Fragments arranged by size: 

                 613     327     277      82
 
BanII G_rGCy'C

Cuts at:      0    1088    1299 
   Size:       1088     211
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     896    1275    1299 
   Size:        896     379      24

  Fragments arranged by size: 

                 896     379      24
 
BbvCI CC'TCA_GC

Cuts at:      0     817    1299 
   Size:        817     482
 
BlpI GC'TnA_GC

Cuts at:      0    1075    1299 
   Size:       1075     224
 
Bme1580I G_kGCm'C

Cuts at:      0     468     752     913    1088    1299 
   Size:        468     284     161     175     211

  Fragments arranged by size: 

                 468     284     211     175     161
 
BmgBI CAC'GTC

Cuts at:      0     429     618    1161    1299 
   Size:        429     189     543     138

  Fragments arranged by size: 

                 543     429     189     138
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     452    1132    1189    1299 
   Size:        452     680      57     110

  Fragments arranged by size: 

                 680     452     110      57
 
Bpu10I CC'TnA_GC

Cuts at:      0     817    1299 
   Size:        817     482
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      76     130     952    1299 
   Size:         76      54     822     347

  Fragments arranged by size: 

                 822     347      76      54
 
BsaAI yAC'GTr

Cuts at:      0     261     287    1299 
   Size:        261      26    1012

  Fragments arranged by size: 

                1012     261      26
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1148    1178    1265    1295    1299 
   Size:       1148      30      87      30       4

  Fragments arranged by size: 

                1148      87      30      30       4
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     112     173    1299 
   Size:        112      61    1126

  Fragments arranged by size: 

                1126     112      61
 
BseYI C'CCAG_C

Cuts at:      0     140    1229    1299 
   Size:        140    1089      70

  Fragments arranged by size: 

                1089     140      70
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      78     991    1299 
   Size:         78     913     308

  Fragments arranged by size: 

                 913     308      78
 
BsiHKAI G_wGCw'C

Cuts at:      0     468     788     913    1076    1299 
   Size:        468     320     125     163     223

  Fragments arranged by size: 

                 468     320     223     163     125
 
BsiWI C'GTAC_G

Cuts at:      0     803    1299 
   Size:        803     496
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1151    1299 
   Size:       1151     148
 
Bsp1286I G_dGCh'C

Cuts at:      0     468     752     788     913    1076    1088    1299 
   Size:        468     284      36     125     163      12     211

  Fragments arranged by size: 

                 468     284     211     163     125      36      12
 
BspHI T'CATG_A

Cuts at:      0     662    1124    1299 
   Size:        662     462     175

  Fragments arranged by size: 

                 662     462     175
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     652    1059    1299 
   Size:        652     407     240

  Fragments arranged by size: 

                 652     407     240
 
BsrBI CCG'CTC

Cuts at:      0     487    1299 
   Size:        487     812
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     472     747    1299 
   Size:        472     275     552

  Fragments arranged by size: 

                 552     472     275
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     513    1299 
   Size:        513     786
 
Bsu36I CC'TnA_GG

Cuts at:      0     964    1299 
   Size:        964     335
 
BtgI C'CryG_G

Cuts at:      0     728    1299 
   Size:        728     571
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      96    1299 
   Size:         96    1203
 
BtsI GCAGTG_nn'

Cuts at:      0      66     188     762    1299 
   Size:         66     122     574     537

  Fragments arranged by size: 

                 574     537     122      66
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     230     265    1299 
   Size:        230      35    1034

  Fragments arranged by size: 

                1034     230      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     747    1299 
   Size:        747     552
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1138    1299 
   Size:       1138     161
 
EaeI y'GGCC_r

Cuts at:      0     107    1299 
   Size:        107    1192
 
EarI CTCTTCn'nnn_

Cuts at:      0      38     293     463     793    1299 
   Size:         38     255     170     330     506

  Fragments arranged by size: 

                 506     330     255     170      38
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     721    1299 
   Size:        721     578
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      79    1051    1299 
   Size:         79     972     248

  Fragments arranged by size: 

                 972     248      79
 
EcoO109I rG'GnC_Cy

Cuts at:      0     641    1001    1085    1299 
   Size:        641     360      84     214

  Fragments arranged by size: 

                 641     360     214      84
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     804    1299 
   Size:        804     495
 
HaeII r_GCGC'y

Cuts at:      0     824    1299 
   Size:        824     475
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     438     470     883     915    1148    1180    1243    1265 
   Size:        438      32     413      32     233      32      63      22

Cuts at:   1265    1275    1297    1299 
   Size:         10      22       2

  Fragments arranged by size: 

                 438     413     233      63      32      32      32      22
                  22      10       2
 
MluI A'CGCG_T

Cuts at:      0     800    1299 
   Size:        800     499
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     905    1251    1299 
   Size:        905     346      48

  Fragments arranged by size: 

                 905     346      48
 
MscI TGG'CCA

Cuts at:      0     109    1299 
   Size:        109    1190
 
MslI CAynn'nnrTG

Cuts at:      0     263     568    1216    1299 
   Size:        263     305     648      83

  Fragments arranged by size: 

                 648     305     263      83
 
MspA1I CmG'CkG

Cuts at:      0     534    1299 
   Size:        534     765
 
NcoI C'CATG_G

Cuts at:      0     728    1299 
   Size:        728     571
 
NspI r_CATG'y

Cuts at:      0    1215    1299 
   Size:       1215      84
 
PciI A'CATG_T

Cuts at:      0    1211    1299 
   Size:       1211      88
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1106    1299 
   Size:       1106     193
 
PfoI T'CCnGG_A

Cuts at:      0     422    1299 
   Size:        422     877
 
PmlI CAC'GTG

Cuts at:      0     261     287    1299 
   Size:        261      26    1012

  Fragments arranged by size: 

                1012     261      26
 
PpuMI rG'GwC_Cy

Cuts at:      0     641    1001    1299 
   Size:        641     360     298

  Fragments arranged by size: 

                 641     360     298
 
PspOMI G'GGCC_C

Cuts at:      0    1084    1299 
   Size:       1084     215
 
PstI C_TGCA'G

Cuts at:      0      80     195     392     545     757    1052    1299 
   Size:         80     115     197     153     212     295     247

  Fragments arranged by size: 

                 295     247     212     197     153     115      80
 
PvuII CAG'CTG

Cuts at:      0     534    1299 
   Size:        534     765
 
SanDI GG'GwC_CC

Cuts at:      0    1001    1299 
   Size:       1001     298
 
SapI GCTCTTCn'nnn_

Cuts at:      0      38     793    1299 
   Size:         38     755     506

  Fragments arranged by size: 

                 755     506      38
 
SbfI CC_TGCA'GG

Cuts at:      0      80    1052    1299 
   Size:         80     972     247

  Fragments arranged by size: 

                 972     247      80
 
ScaI AGT'ACT

Cuts at:      0     304    1299 
   Size:        304     995
 
SfcI C'TryA_G

Cuts at:      0      76     191     388     541     753    1048    1299 
   Size:         76     115     197     153     212     295     251

  Fragments arranged by size: 

                 295     251     212     197     153     115      76
 
SmaI CCC'GGG

Cuts at:      0     688    1299 
   Size:        688     611
 
StyI C'CwwG_G

Cuts at:      0     728    1299 
   Size:        728     571
 
TatI w'GTAC_w

Cuts at:      0      87     302    1299 
   Size:         87     215     997

  Fragments arranged by size: 

                 997     215      87
 
Tth111I GACn'n_nGTC

Cuts at:      0     712    1138    1162    1299 
   Size:        712     426      24     137

  Fragments arranged by size: 

                 712     426     137      24
 
XbaI T'CTAG_A

Cuts at:      0     954    1299 
   Size:        954     345
 
XmaI C'CCGG_G

Cuts at:      0     686    1299 
   Size:        686     613
 
XmnI GAAnn'nnTTC

Cuts at:      0     167    1299 
   Size:        167    1132
 

 Enzymes that do cut:

     AarI     AcuI     AfeI   AflIII     AlfI    AlwNI     ApaI    ApaLI
     ApoI     AvaI    BanII     BbsI    BbvCI     BlpI Bme1580I    BmgBI
     BmrI   Bpu10I     BsaI    BsaAI    BsaXI    BseRI    BseYI     BsgI
  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspHI    BspMI    BsrBI   BstAPI
    BstXI   Bsu36I     BtgI    BtgZI     BtsI    CspCI   DraIII     DrdI
     EaeI     EarI  Eco57MI    EcoNI EcoO109I     FalI    HaeII    Hin4I
     MluI     MmeI     MscI     MslI   MspA1I     NcoI     NspI     PciI
    PflMI     PfoI     PmlI    PpuMI   PspOMI     PstI    PvuII    SanDI
     SapI     SbfI     ScaI     SfcI     SmaI     StyI     TatI  Tth111I
     XbaI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI    AflII     AgeI     AhdI     AleI
     AloI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI
     BbeI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BmtI
     BplI     BpmI    BpuEI    BsaBI    BsaHI    BsaWI    BsiEI     BsmI
    BspEI    BsrDI    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII
    BstYI  BstZ17I     ClaI     DraI     EagI     EciI  EcoICRI    EcoRI
    EcoRV     FseI     FspI    FspAI   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI     PmeI     PpiI    PshAI     PsiI
    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI    TaqII     TstI     XcmI     XhoI
     ZraI
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