Restriction map for pF1KB8133
##################################
# NO CUT ENZYMES (  115 enzymes) #
##################################

AccIII      AclI        AcsI        AflII       AgeI        
AhdI        Alw44I      AocI        Aor51HI     ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
AspEI       AspHI       AvrII       BamHI       BbrPI       
BciVI       BclI        BfrI        BlnI        BlpI        
Bpu1102I    BsaWI       BseAI       BsiHKAI     BsiWI       
BsmBI       BsmI        Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrBI       BsrDI       BsrGI       
BssHII      Bst1107I    BstBI       BstXI       BstZ17I     
Bsu36I      CelII       ClaI        CpoI        DraI        
DraIII      Eam1105I    EarI        Eco47III    Eco81I      
EcoNI       EcoRI       EcoT22I     FbaI        FseI        
HindIII     HpaI        Ksp632I     KspI        MfeI        
MluI        MroI        MseI        MunI        NaeI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SfiI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        Sse8387I    SspBI       SspI        
StuI        SwaI        TfiI        Tru9I       Tsp509I     
XbaI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   33 enzymes) #
###################################

AatII        1432  GACGTC               GACGT^C
AccI           40  GTCTAC               GT^[AC][GT]AC
AflIII        569  ACGTGT               A^C[GA][CT]GT
AviII        1383  TGCGCA               TGC^GCA
BbeI         1033  GGCGCC               GGCGC^C
BbsI         1549  GTCTTC               GAAGAC(2/6)
BbvCI         291  CCTCAGC              CCTCAGC(-5/-2)
BglI         1453  GCCAGCGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI        1549  GTCTTC               GAAGAC(2/6)
BsaAI        1252  TACGTG               [CT]AC^GT[GA]
BsaBI        1092  GATCGGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI          68  CGGCCG               CG[GA][CT]^CG
BssSI        1525  CACGAG               CACGAG(-5/-1)
BstEII        808  GGTGACC              G^GT[ACGT]ACC
BstPI         808  GGTGACC              G^GT[ACGT]ACC
DrdI           31  GACAGCCTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI           68  CGGCCG               C^GGCCG
EclXI          68  CGGCCG               C^GGCCG
Eco52I         68  CGGCCG               C^GGCCG
EcoO65I       808  GGTGACC              G^GT[ACGT]ACC
EcoRV         631  GATATC               GAT^ATC
FspI         1383  TGCGCA               TGC^GCA
KasI         1033  GGCGCC               G^GCGCC
MaeIII        809  GTGAC                ^GT[ACGT]AC
MamI         1092  GATCGGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI         1033  GGCGCC               GG^CGCC
PpuMI         281  AGGTCCT              [GA]G^G[AT]CC[CT]
PvuII         949  CAGCTG               CAG^CTG
SfoI         1033  GGCGCC               GGC^GCC
SmlI         1348  CTCAAG               C^T[CT][GA]AG
SphI          962  GCATGC               GCATG^C
Tsp45I        809  GTGAC                ^GT[GC]AC
XcmI         1274  CCAACGGTGCGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

Acc65I        311  GGTACC               G^GTACC
             1244  GGTACC              
AlwI          720  GATCC                GGATC(4/5)
             1107  GATCC               
ApaI           63  GGGCCC               GGGCC^C
             1135  GGGCCC              
Asp718I       311  GGTACC               G^GTACC
             1244  GGTACC              
AspI          277  GACCAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1186  GACCACGTC           
BalI         1451  TGGCCA               TGG^CCA
             1511  TGGCCA              
BfaI          523  CTAG                 C^TAG
              967  CTAG                
BglII          47  AGATCT               A^GATCT
              350  AGATCT              
BsaI          592  GAGACC               GGTCTC(1/5)
              970  GAGACC              
BsmAI         592  GAGAC                GTCTC(1/5)
              970  GAGAC               
BsrFI         805  GCCGGT               [GA]^CCGG[CT]
             1469  ACCGGC              
Cfr10I        805  GCCGGT               [GA]^CCGG[CT]
             1469  ACCGGC              
DraII         281  AGGTCCT              [GA]G^G[ACGT]CC[CT]
             1134  AGGGCCC             
DsaI         1127  CCACGG               C^C[GA][CT]GG
             1654  CCACGG              
EcoO109I      281  AGGTCCT              [GA]G^G[ACGT]CC[CT]
             1134  AGGGCCC             
HaeII        1033  GGCGCC               [GA]GCGC^[CT]
             1613  GGCGCT              
HincII        694  GTCAAC               GT[CT]^[GA]AC
             1537  GTCAAC              
HindII        694  GTCAAC               GT[CT]^[GA]AC
             1537  GTCAAC              
KpnI          311  GGTACC               GGTAC^C
             1244  GGTACC              
MaeI          523  CTAG                 C^TAG
              967  CTAG                
MluNI        1451  TGGCCA               TGG^CCA
             1511  TGGCCA              
MscI         1451  TGGCCA               TGG^CCA
             1511  TGGCCA              
NspI          395  ACATGC               [GA]CATG^[CT]
              962  GCATGC              
PflFI         277  GACCAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1186  GACCACGTC           
PflMI         197  CCATGTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              672  CCAGGATGTGG         
PstI          768  CTGCAG               CTGCA^G
             1519  CTGCAG              
SexAI         161  ACCAGGT              A^CC[AT]GGT
              278  ACCAGGT             
SfcI          768  CTGCAG               C^T[GA][CT]AG
             1519  CTGCAG              
Tth111I       277  GACCAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1186  GACCACGTC           
Van91I        197  CCATGTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              672  CCAGGATGTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   80 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwNI       AvaI        AvaII       BanI        BanII       
BbvI        BcgI        BcnI        BmyI        BpmI        
BsaHI       BsaJI       BseRI       BsgI        BsiYI       
BslI        BsmFI       BsoBI       Bsp1286I    BspMI       
BsrI        BssKI       BstAPI      BstF5I      BstNI       
BstUI       BstYI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       EaeI        EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HinfI       
HpaII       HphI        ItaI        MaeII       MboI        
MboII       MflI        MnlI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PleI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       StyI        
TaqI        TseI        TspRI       TthHB8I     XhoII       
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