Restriction map for pF1KB8122
 (Linear) (Six-base) MAPSORT of: KIBB8122.seq  Check: 1507  from: 1  to: 642



KIBB8122.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:03  ..

AclI AA'CG_TT

Cuts at:      0     357     642 
   Size:        357     285
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      78     642 
   Size:         78     564
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     148     642 
   Size:        148     494
 
BanI G'GyrC_C

Cuts at:      0     501     642 
   Size:        501     141
 
BanII G_rGCy'C

Cuts at:      0      68     589     642 
   Size:         68     521      53

  Fragments arranged by size: 

                 521      68      53
 
BfrBI ATG'CAT

Cuts at:      0     177     642 
   Size:        177     465
 
Bme1580I G_kGCm'C

Cuts at:      0     506     642 
   Size:        506     136
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     295     642 
   Size:        295     347
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     127     232     484     642 
   Size:        127     105     252     158

  Fragments arranged by size: 

                 252     158     127     105
 
Bpu10I CC'TnA_GC

Cuts at:      0     327     642 
   Size:        327     315
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     276     642 
   Size:        276     366
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     184     400     403     406     424     427     481     642 
   Size:        184     216       3       3      18       3      54     161

  Fragments arranged by size: 

                 216     184     161      54      18       3       3       3
            
 
BseYI C'CCAG_C

Cuts at:      0     101     198     642 
   Size:        101      97     444

  Fragments arranged by size: 

                 444     101      97
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     307     642 
   Size:        307     335
 
BsiEI CG_ry'CG

Cuts at:      0      47     642 
   Size:         47     595
 
BsiHKAI G_wGCw'C

Cuts at:      0      53      68     642 
   Size:         53      15     574

  Fragments arranged by size: 

                 574      53      15
 
Bsp1286I G_dGCh'C

Cuts at:      0      53      68     506     589     642 
   Size:         53      15     438      83      53

  Fragments arranged by size: 

                 438      83      53      53      15
 
BsrDI GCAATG_nn'

Cuts at:      0     236     642 
   Size:        236     406
 
BsrFI r'CCGG_y

Cuts at:      0     228     642 
   Size:        228     414
 
BstEII G'GTnAC_C

Cuts at:      0     231     642 
   Size:        231     411
 
BstYI r'GATC_y

Cuts at:      0     188     642 
   Size:        188     454
 
BtgI C'CryG_G

Cuts at:      0     266     275     642 
   Size:        266       9     367

  Fragments arranged by size: 

                 367     266       9
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     163     642 
   Size:        163     479
 
BtsI GCAGTG_nn'

Cuts at:      0      44     642 
   Size:         44     598
 
EaeI y'GGCC_r

Cuts at:      0     348     642 
   Size:        348     294
 
EarI CTCTTCn'nnn_

Cuts at:      0     413     419     548     642 
   Size:        413       6     129      94

  Fragments arranged by size: 

                 413     129      94       6
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      96     642 
   Size:         96     546
 
EcoICRI GAG'CTC

Cuts at:      0      66     642 
   Size:         66     576
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      78     127     232     484     642 
   Size:         78      49     105     252     158

  Fragments arranged by size: 

                 252     158     105      78      49
 
EcoO109I rG'GnC_Cy

Cuts at:      0     331     642 
   Size:        331     311
 
HaeII r_GCGC'y

Cuts at:      0      17     642 
   Size:         17     625
 
MscI TGG'CCA

Cuts at:      0     350     642 
   Size:        350     292
 
MspA1I CmG'CkG

Cuts at:      0     105     642 
   Size:        105     537
 
NcoI C'CATG_G

Cuts at:      0     266     275     642 
   Size:        266       9     367

  Fragments arranged by size: 

                 367     266       9
 
NsiI A_TGCA'T

Cuts at:      0     179     642 
   Size:        179     463
 
PpuMI rG'GwC_Cy

Cuts at:      0     331     642 
   Size:        331     311
 
PstI C_TGCA'G

Cuts at:      0     127     642 
   Size:        127     515
 
PvuII CAG'CTG

Cuts at:      0     105     642 
   Size:        105     537
 
SacI G_AGCT'C

Cuts at:      0      68     642 
   Size:         68     574
 
SfcI C'TryA_G

Cuts at:      0     123     642 
   Size:        123     519
 
StuI AGG'CCT

Cuts at:      0     253     642 
   Size:        253     389
 
StyI C'CwwG_G

Cuts at:      0     266     275     642 
   Size:        266       9     367

  Fragments arranged by size: 

                 367     266       9
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     273     319     642 
   Size:        273      46     323

  Fragments arranged by size: 

                 323     273      46
 

 Enzymes that do cut:

     AclI     AcuI     AlfI     BanI    BanII    BfrBI Bme1580I     BmrI
     BpmI   Bpu10I     BsaI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
 Bsp1286I    BsrDI    BsrFI   BstEII    BstYI     BtgI    BtgZI     BtsI
     EaeI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    HaeII     MscI
   MspA1I     NcoI     NsiI    PpuMI     PstI    PvuII     SacI     SfcI
     StuI     StyI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI     BglI    BglII     BlpI    BmgBI
     BmtI     BplI    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrGI
   BssHII    BssSI   BstAPI    BstBI    BstXI  BstZ17I   Bsu36I     ClaI
    CspCI     DraI   DraIII     DrdI     EagI    EcoNI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    Hin4I   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MslI     NaeI     NarI
     NdeI   NgoMIV     NheI     NotI     NruI     NspI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    RsrII    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI
    TaqII     TatI     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI
     ZraI
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