Restriction map for pF1KB8119
##################################
# NO CUT ENZYMES (  138 enzymes) #
##################################

Acc65I      AccIII      AclI        AcsI        AfaI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AviII       AvrII       BamHI       
BanI        BbeI        BbrPI       BbsI        BbvCI       
BciVI       BclI        BfaI        BfrI        BglII       
BlnI        BpuAI       BsaAI       BsaBI       BsaI        
BsaWI       BseAI       BseRI       BsgI        BsiHKAI     
BsiWI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BsrDI       BsrGI       Bst1107I    
BstAPI      BstBI       BstEII      BstF5I      BstPI       
BstYI       BstZ17I     Bsu36I      ClaI        CpoI        
DraI        DraIII      DrdI        Eam1105I    EarI        
Eco47III    Eco81I      EcoNI       EcoO65I     EcoRI       
EcoRV       EcoT22I     FbaI        FokI        FspI        
HindIII     HpaI        KasI        KpnI        Ksp632I     
MaeI        MamI        MfeI        MflI        MluI        
MroI        MseI        MslI        MunI        NarI        
NcoI        NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PshBI       Psp1406I    PvuI        PvuII       RcaI        
RsaI        RsrII       SacI        SapI        ScaI        
SexAI       SfaNI       SfiI        SfoI        SfuI        
SgrAI       SmlI        SnaBI       SpeI        SphI        
SspBI       SwaI        Tru9I       Tth111I     XbaI        
XhoI        XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   57 enzymes) #
###################################

AatII         259  GACGTC               GACGT^C
AccI           54  GTCGAC               GT^[AC][GT]AC
AluI          332  AGCT                 AG^CT
AlwI          421  GATCC                GGATC(4/5)
AlwNI         458  CAGGAACTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI          280  GGGCCC               GGGCC^C
AvaI          285  CCCGGG               C^[CT]CG[GA]G
BalI          212  TGGCCA               TGG^CCA
BcgI          499  GCAAAGCATTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI           43  GCCCTGCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI          270  GCTCAGC              GC^T[ACGT]AGC
BpmI          229  CTGGAG               CTGGAG(16/14)
Bpu1102I      270  GCTCAGC              GC^T[ACGT]AGC
BsmAI         193  GTCTC                GTCTC(1/5)
BsmBI         192  CGTCTC               CGTCTC(1/5)
BsmI          504  GCATTC               GAATGC(1/-1)
BsoBI         285  CCCGGG               C^[CT]CG[GA]G
BspMI         347  ACCTGC               ACCTGC(4/8)
BsrFI         245  GCCGGC               [GA]^CCGG[CT]
BssSI          73  CTCGTG               CACGAG(-5/-1)
BstXI         377  CCATGACTTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII         270  GCTCAGC              GC^T[ACGT]AGC
Cfr10I        245  GCCGGC               [GA]^CCGG[CT]
DpnII         421  GATC                 ^GATC
EcoT14I       470  CCAAGG               C^C[AT][AT]GG
FseI          244  GGCCGGCC             GGCCGG^CC
HincII         54  GTCGAC               GT[CT]^[GA]AC
HindII         54  GTCGAC               GT[CT]^[GA]AC
HinfI         357  GAATC                G^A[ACGT]TC
HphI          474  GGTGA                GGTGA(8/7)
KspI           15  CCGCGG               CCGC^GG
MboI          421  GATC                 ^GATC
MboII         634  GAAGA                GAAGA(8/7)
MluNI         212  TGGCCA               TGG^CCA
MscI          212  TGGCCA               TGG^CCA
NaeI          245  GCCGGC               GCC^GGC
NdeI          362  CATATG               CA^TATG
NdeII         421  GATC                 ^GATC
NgoMIV        245  GCCGGC               G^CCGGC
PflMI         584  CCAATATCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI         593  GGGTCCT              [GA]G^G[AT]CC[CT]
PshAI         186  GACGCACGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII          15  CCGCGG               CCGC^GG
SalI           54  GTCGAC               G^TCGAC
Sau3AI        421  GATC                 ^GATC
SbfI          348  CCTGCAGG             CCTGCA^GG
SmaI          285  CCCGGG               CCC^GGG
Sse8387I      348  CCTGCAGG             CCTGCA^GG
SspI          638  AATATT               AAT^ATT
StuI          125  AGGCCT               AGG^CCT
StyI          470  CCAAGG               C^C[AT][AT]GG
TfiI          357  GAATC                G^A[AT]TC
Tsp509I       556  AATT                 ^AATT
Van91I        584  CCAATATCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          218  CCATCTCCACGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI         285  CCCGGG               C^CCGGG
XmaI          285  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AcyI           51  GGCGTC               G[GA]^CG[CT]C
              259  GACGTC              
BanII         280  GGGCCC               G[GA]GC[CT]^C
              321  GAGCCC              
BsaHI          51  GGCGTC               G[GA]^CG[CT]C
              259  GACGTC              
BsiEI          99  CGGCCG               CG[GA][CT]^CG
              243  CGGCCG              
BsrI          116  ACTGG                ACTGG(1/-1)
              200  ACTGG               
BstNI         539  CCTGG                CC^[AT]GG
              597  CCTGG               
DraII         280  GGGCCCC              [GA]G^G[ACGT]CC[CT]
              593  GGGTCCT             
DsaI           15  CCGCGG               C^C[GA][CT]GG
              108  CCACGG              
EagI           99  CGGCCG               C^GGCCG
              243  CGGCCG              
EclXI          99  CGGCCG               C^GGCCG
              243  CGGCCG              
Eco52I         99  CGGCCG               C^GGCCG
              243  CGGCCG              
EcoO109I      280  GGGCCCC              [GA]G^G[ACGT]CC[CT]
              593  GGGTCCT             
EcoRII        539  CCTGG                ^CC[AT]GG
              597  CCTGG               
HaeII          66  GGCGCT               [GA]GCGC^[CT]
              205  GGCGCT              
Hin1I          51  GGCGTC               G[GA]^CG[CT]C
              259  GACGTC              
MaeII         191  ACGT                 A^CGT
              260  ACGT                
MvaI          539  CCTGG                CC^[AT]GG
              597  CCTGG               
NlaIII        378  CATG                 CATG^
              412  CATG                
PstI          328  CTGCAG               CTGCA^G
              349  CTGCAG              
SfcI          328  CTGCAG               C^T[GA][CT]AG
              349  CTGCAG              
TaqI           55  TCGA                 T^CGA
              519  TCGA                
TspRI          32  CACTG                CA[GC]TG[ACGT][ACGT]^
              371  CACTG               
TthHB8I        55  TCGA                 T^CGA
              519  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   40 enzymes) #
#########################################

AccII       AciI        AvaII       BbvI        BcnI        
BmyI        BsaJI       BsiYI       BslI        BsmFI       
Bsp1286I    BssHII      BssKI       BstUI       Cac8I       
CfoI        Cfr13I      DdeI        EaeI        FauI        
Fnu4HI      HaeIII      HapII       HgaI        HhaI        
HinP1I      HpaII       ItaI        MaeIII      MnlI        
MspA1I      MspI        MvnI        MwoI        NciI        
NlaIV       Sau96I      ScrFI       TseI        Tsp45I      
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