Restriction map for pF1KB8119
 (Linear) (Six-base) MAPSORT of: KIBB8119.seq  Check: 5779  from: 1  to: 660



KIBB8119.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:03  ..

AatII G_ACGT'C

Cuts at:      0     263     660 
   Size:        263     397
 
AccI GT'mk_AC

Cuts at:      0      55     660 
   Size:         55     605
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     319     660 
   Size:        319     341
 
AlwNI CAG_nnn'CTG

Cuts at:      0     463     660 
   Size:        463     197
 
ApaI G_GGCC'C

Cuts at:      0     284     660 
   Size:        284     376
 
AvaI C'yCGr_G

Cuts at:      0     285     660 
   Size:        285     375
 
BanII G_rGCy'C

Cuts at:      0     284     325     660 
   Size:        284      41     335

  Fragments arranged by size: 

                 335     284      41
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     488     522     660 
   Size:        488      34     138

  Fragments arranged by size: 

                 488     138      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0      49     660 
   Size:         49     611
 
BlpI GC'TnA_GC

Cuts at:      0     271     660 
   Size:        271     389
 
Bme1580I G_kGCm'C

Cuts at:      0     284     308     660 
   Size:        284      24     352

  Fragments arranged by size: 

                 352     284      24
 
BmgBI CAC'GTC

Cuts at:      0     192     660 
   Size:        192     468
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     126     210     660 
   Size:        126      84     450

  Fragments arranged by size: 

                 450     126      84
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     250     660 
   Size:        250     410
 
Bpu10I CC'TnA_GC

Cuts at:      0     319     660 
   Size:        319     341
 
BsaHI Gr'CG_yC

Cuts at:      0      52     260     660 
   Size:         52     208     400

  Fragments arranged by size: 

                 400     208      52
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     112     142     660 
   Size:        112      30     518

  Fragments arranged by size: 

                 518     112      30
 
BsiEI CG_ry'CG

Cuts at:      0     102     246     660 
   Size:        102     144     414

  Fragments arranged by size: 

                 414     144     102
 
BsmI GAATG_Cn'

Cuts at:      0     504     660 
   Size:        504     156
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     198     660 
   Size:        198     462
 
Bsp1286I G_dGCh'C

Cuts at:      0     284     308     325     660 
   Size:        284      24      17     335

  Fragments arranged by size: 

                 335     284      24      17
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     356     660 
   Size:        356     304
 
BsrFI r'CCGG_y

Cuts at:      0     245     660 
   Size:        245     415
 
BssHII G'CGCG_C

Cuts at:      0      25      27     166     660 
   Size:         25       2     139     494

  Fragments arranged by size: 

                 494     139      25       2
 
BssSI C'ACGA_G

Cuts at:      0      73     660 
   Size:         73     587
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     384     660 
   Size:        384     276
 
BtgI C'CryG_G

Cuts at:      0      15     108     660 
   Size:         15      93     552

  Fragments arranged by size: 

                 552      93      15
 
BtsI GCAGTG_nn'

Cuts at:      0      31     660 
   Size:         31     629
 
EaeI y'GGCC_r

Cuts at:      0       2      99     212     243     660 
   Size:          2      97     113      31     417

  Fragments arranged by size: 

                 417     113      97      31       2
 
EagI C'GGCC_G

Cuts at:      0      99     243     660 
   Size:         99     144     417

  Fragments arranged by size: 

                 417     144      99
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     250     660 
   Size:        250     410
 
EcoO109I rG'GnC_Cy

Cuts at:      0     281     594     660 
   Size:        281     313      66

  Fragments arranged by size: 

                 313     281      66
 
FseI GG_CCGG'CC

Cuts at:      0     249     660 
   Size:        249     411
 
HaeII r_GCGC'y

Cuts at:      0      70     209     660 
   Size:         70     139     451

  Fragments arranged by size: 

                 451     139      70
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     112     144     250     282     605     637     660 
   Size:        112      32     106      32     323      32      23

  Fragments arranged by size: 

                 323     112     106      32      32      32      23
 
HincII GTy'rAC

Cuts at:      0      56     660 
   Size:         56     604
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     221     660 
   Size:        221     439
 
MscI TGG'CCA

Cuts at:      0     214     660 
   Size:        214     446
 
MspA1I CmG'CkG

Cuts at:      0      17     299     327     660 
   Size:         17     282      28     333

  Fragments arranged by size: 

                 333     282      28      17
 
NaeI GCC'GGC

Cuts at:      0     247     660 
   Size:        247     413
 
NdeI CA'TA_TG

Cuts at:      0     363     660 
   Size:        363     297
 
NgoMIV G'CCGG_C

Cuts at:      0     245     660 
   Size:        245     415
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     590     660 
   Size:        590      70
 
PfoI T'CCnGG_A

Cuts at:      0     596     660 
   Size:        596      64
 
PpuMI rG'GwC_Cy

Cuts at:      0     594     660 
   Size:        594      66
 
PshAI GACnn'nnGTC

Cuts at:      0     190     660 
   Size:        190     470
 
PsiI TTA'TAA

Cuts at:      0     644     660 
   Size:        644      16
 
PspOMI G'GGCC_C

Cuts at:      0     280     660 
   Size:        280     380
 
PstI C_TGCA'G

Cuts at:      0     332     353     660 
   Size:        332      21     307

  Fragments arranged by size: 

                 332     307      21
 
SacII CC_GC'GG

Cuts at:      0      18     660 
   Size:         18     642
 
SalI G'TCGA_C

Cuts at:      0      54     660 
   Size:         54     606
 
SbfI CC_TGCA'GG

Cuts at:      0     353     660 
   Size:        353     307
 
SfcI C'TryA_G

Cuts at:      0     328     349     660 
   Size:        328      21     311

  Fragments arranged by size: 

                 328     311      21
 
SmaI CCC'GGG

Cuts at:      0     287     660 
   Size:        287     373
 
SspI AAT'ATT

Cuts at:      0     640     660 
   Size:        640      20
 
StuI AGG'CCT

Cuts at:      0     127     660 
   Size:        127     533
 
StyI C'CwwG_G

Cuts at:      0     470     660 
   Size:        470     190
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     225     660 
   Size:        225     435
 
XmaI C'CCGG_G

Cuts at:      0     285     660 
   Size:        285     375
 
ZraI GAC'GTC

Cuts at:      0     261     660 
   Size:        261     399
 

 Enzymes that do cut:

    AatII     AccI     AlfI    AlwNI     ApaI     AvaI    BanII     BcgI
     BglI     BlpI Bme1580I    BmgBI     BmrI     BpmI   Bpu10I    BsaHI
    BsaXI    BsiEI     BsmI    BsmBI Bsp1286I    BspMI    BsrFI   BssHII
    BssSI    BstXI     BtgI     BtsI     EaeI     EagI  Eco57MI EcoO109I
     FseI    HaeII    Hin4I   HincII     MmeI     MscI   MspA1I     NaeI
     NdeI   NgoMIV    PflMI     PfoI    PpuMI    PshAI     PsiI   PspOMI
     PstI    SacII     SalI     SbfI     SfcI     SmaI     SspI     StuI
     StyI     XcmI     XmaI     ZraI

 Enzymes that do not cut: 

     AarI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AloI    ApaLI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BanI     BbeI     BbsI    BbvCI    BciVI
     BclI    BfrBI    BglII     BmtI     BplI    BpuEI     BsaI    BsaAI
    BsaBI    BsaWI    BseRI    BseYI     BsgI  BsiHKAI    BsiWI    BspEI
    BspHI    BsrBI    BsrDI    BsrGI   BstAPI    BstBI   BstEII    BstYI
  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI
     EarI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FspI
    FspAI  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MslI
     NarI     NcoI     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI     PmeI     PmlI     PpiI    PspXI     PsrI     PvuI
    PvuII    RsrII     SacI    SanDI     SapI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI     SrfI
     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XhoI     XmnI
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