Restriction map for pF1KB8117
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# NO CUT ENZYMES (  115 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflII       
AflIII      AgeI        AhdI        Alw44I      Aor51HI     
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AviII       AvrII       
BalI        BbvCI       BcgI        BciVI       BfrI        
BlnI        BsaBI       BsaWI       BseAI       BsiEI       
BsiWI       BsmBI       BsmI        Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrBI       BsrDI       
BsrGI       BssHII      BssSI       Bst1107I    BstEII      
BstPI       BstXI       BstZ17I     ClaI        CpoI        
DraI        DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoNI       EcoO65I     EcoRI       
EcoRV       EcoT14I     EcoT22I     FseI        FspI        
HgaI        HpaI        KpnI        KspI        MamI        
MfeI        MluI        MluNI       MroI        MscI        
MunI        NcoI        NdeI        NheI        NotI        
NruI        NsiI        NspI        PacI        PaeR7I      
PflFI       PinAI       PmeI        PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacI        
SacII       SapI        SbfI        ScaI        SexAI       
SfiI        SgrAI       SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StyI        
SwaI        Tth111I     XbaI        XhoI        XmnI        


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# ONE CUT ENZYMES (   42 enzymes) #
###################################

AccI         1099  GTCGAC               GT^[AC][GT]AC
AccII           8  CGCG                 CG^CG
AcsI          425  AAATTC               [GA]^AATT[CT]
AcyI          246  GGCGCC               G[GA]^CG[CT]C
AocI         1444  CCTCAGG              CC^T[ACGT]AGG
ApaI          416  GGGCCC               GGGCC^C
ApoI          425  AAATTC               [GA]^AATT[CT]
BbeI          246  GGCGCC               GGCGC^C
BbrPI         190  CACGTG               CAC^GTG
BclI          252  TGATCA               T^GATCA
BglII         119  AGATCT               A^GATCT
BsaAI         190  CACGTG               [CT]AC^GT[GA]
BsaHI         246  GGCGCC               G[GA]^CG[CT]C
BsaI          781  GGTCTC               GGTCTC(1/5)
BsgI         1022  GTGCAG               GTGCAG(16/14)
BstAPI        936  GCAGGTGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI         842  TTCGAA               TT^CGAA
BstUI           8  CGCG                 CG^CG
Bsu36I       1444  CCTCAGG              CC^T[ACGT]AGG
DraIII        379  CACGCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI         1031  CCACGG               C^C[GA][CT]GG
EarI         1197  GAAGAG               CTCTTC(1/4)
Eco81I       1444  CCTCAGG              CC^T[ACGT]AGG
FbaI          252  TGATCA               T^GATCA
Hin1I         246  GGCGCC               G[GA]^CG[CT]C
HincII       1099  GTCGAC               GT[CT]^[GA]AC
HindII       1099  GTCGAC               GT[CT]^[GA]AC
HindIII      1126  AAGCTT               A^AGCTT
KasI          246  GGCGCC               G^GCGCC
Ksp632I      1197  GAAGAG               CTCTTC(1/4)
MseI          713  TTAA                 T^TAA
MvnI            8  CGCG                 CG^CG
NarI          246  GGCGCC               GG^CGCC
NspV          842  TTCGAA               TT^CGAA
PmaCI         190  CACGTG               CAC^GTG
PmlI          190  CACGTG               CAC^GTG
PvuII         143  CAGCTG               CAG^CTG
SalI         1099  GTCGAC               G^TCGAC
SfoI          246  GGCGCC               GGC^GCC
SfuI          842  TTCGAA               TT^CGAA
Tru9I         713  TTAA                 T^TAA
Tsp509I       426  AATT                 ^AATT


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AfaI          480  GTAC                 GT^AC
              640  GTAC                
BamHI         762  GGATCC               G^GATCC
             1440  GGATCC              
BanI          246  GGCGCC               G^G[CT][GA]CC
              354  GGTGCC              
BanII         416  GGGCCC               G[GA]GC[CT]^C
              746  GAGCCC              
BseRI         436  GAGGAG               GAGGAG(10/8)
             1390  GAGGAG              
BsmFI         344  GGGAC                GGGAC(10/14)
              805  GTCCC               
HaeII          17  GGCGCT               [GA]GCGC^[CT]
              246  GGCGCC              
MslI          531  CACAGCCGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1224  CACAACCATG          
NaeI          680  GCCGGC               GCC^GGC
              867  GCCGGC              
NgoMIV        680  GCCGGC               G^CCGGC
              867  GCCGGC              
NlaIII       1041  CATG                 CATG^
             1230  CATG                
PflMI         378  CCACGCTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              501  CCAGGAGCTGG         
PleI         1120  GACTC                GAGTC(4/5)
              651  GAGTC               
PstI         1182  CTGCAG               CTGCA^G
             1264  CTGCAG              
RsaI          480  GTAC                 GT^AC
              640  GTAC                
SmaI          461  CCCGGG               CCC^GGG
             1400  CCCGGG              
TfiI          540  GATTC                G^A[AT]TC
              732  GATTC               
Van91I        378  CCACGCTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              501  CCAGGAGCTGG         
XcmI         1031  CCACGGGAGACATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1424  CCAGGGAGAAGCTGG     
XmaCI         461  CCCGGG               C^CCGGG
             1400  CCCGGG              
XmaI          461  CCCGGG               C^CCGGG
             1400  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   80 enzymes) #
#########################################

AciI        AluI        AlwI        AlwNI       AspHI       
AvaI        AvaII       BbsI        BbvI        BcnI        
BfaI        BglI        BlpI        BmyI        BpmI        
Bpu1102I    BpuAI       BsaJI       BsiHKAI     BsiYI       
BslI        BsmAI       BsoBI       Bsp1286I    BspMI       
BsrFI       BsrI        BssKI       BstF5I      BstNI       
BstYI       Cac8I       CelII       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       EaeI        
EcoO109I    EcoRII      FauI        Fnu4HI      FokI        
HaeIII      HapII       HhaI        HinP1I      HinfI       
HpaII       HphI        ItaI        MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MspA1I      MspI        MvaI        MwoI        NciI        
NdeII       NlaIV       PpuMI       Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        StuI        TaqI        
TseI        Tsp45I      TspRI       TthHB8I     XhoII       
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