Restriction map for pF1KB8117
 (Linear) (Six-base) MAPSORT of: KIBB8117.seq  Check: 845  from: 1  to: 1473



KIBB8117.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:03  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     176     927     961    1473 
   Size:        176     751      34     512

  Fragments arranged by size: 

                 751     512     176      34
 
AccI GT'mk_AC

Cuts at:      0    1100    1473 
   Size:       1100     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1294    1473 
   Size:       1294     179
 
AleI CACnn'nnGTG

Cuts at:      0     535    1473 
   Size:        535     938
 
AlwNI CAG_nnn'CTG

Cuts at:      0     507     942    1190    1260    1272    1329    1473 
   Size:        507     435     248      70      12      57     144

  Fragments arranged by size: 

                 507     435     248     144      70      57      12
 
ApaI G_GGCC'C

Cuts at:      0     420    1473 
   Size:        420    1053
 
ApoI r'AATT_y

Cuts at:      0     425    1473 
   Size:        425    1048
 
AvaI C'yCGr_G

Cuts at:      0      75     259     461     813    1400    1473 
   Size:         75     184     202     352     587      73

  Fragments arranged by size: 

                 587     352     202     184      75      73
 
BamHI G'GATC_C

Cuts at:      0     762    1440    1473 
   Size:        762     678      33

  Fragments arranged by size: 

                 762     678      33
 
BanI G'GyrC_C

Cuts at:      0     246     354    1473 
   Size:        246     108    1119

  Fragments arranged by size: 

                1119     246     108
 
BanII G_rGCy'C

Cuts at:      0     420     750    1473 
   Size:        420     330     723

  Fragments arranged by size: 

                 723     420     330
 
BbeI G_GCGC'C

Cuts at:      0     250    1473 
   Size:        250    1223
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     115     667    1052    1473 
   Size:        115     552     385     421

  Fragments arranged by size: 

                 552     421     385     115
 
BclI T'GATC_A

Cuts at:      0     252    1473 
   Size:        252    1221
 
BglI GCCn_nnn'nGGC

Cuts at:      0     146     886    1324    1466    1473 
   Size:        146     740     438     142       7

  Fragments arranged by size: 

                 740     438     146     142       7
 
BglII A'GATC_T

Cuts at:      0     119    1473 
   Size:        119    1354
 
BlpI GC'TnA_GC

Cuts at:      0     391    1069    1333    1473 
   Size:        391     678     264     140

  Fragments arranged by size: 

                 678     391     264     140
 
Bme1580I G_kGCm'C

Cuts at:      0     221     420    1031    1115    1414    1473 
   Size:        221     199     611      84     299      59

  Fragments arranged by size: 

                 611     299     221     199      84      59
 
BmgBI CAC'GTC

Cuts at:      0     348     570    1473 
   Size:        348     222     903

  Fragments arranged by size: 

                 903     348     222
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1380    1473 
   Size:       1380      93
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     529     625     769    1018    1473 
   Size:        529      96     144     249     455

  Fragments arranged by size: 

                 529     455     249     144      96
 
Bpu10I CC'TnA_GC

Cuts at:      0     520     596    1473 
   Size:        520      76     877

  Fragments arranged by size: 

                 877     520      76
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     787    1473 
   Size:        787     686
 
BsaAI yAC'GTr

Cuts at:      0     192    1473 
   Size:        192    1281
 
BsaHI Gr'CG_yC

Cuts at:      0     247    1473 
   Size:        247    1226
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      79     109    1473 
   Size:         79      30    1364

  Fragments arranged by size: 

                1364      79      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     451    1405    1473 
   Size:        451     954      68

  Fragments arranged by size: 

                 954     451      68
 
BseYI C'CCAG_C

Cuts at:      0     141     932    1190    1279    1473 
   Size:        141     791     258      89     194

  Fragments arranged by size: 

                 791     258     194     141      89
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1043    1473 
   Size:       1043     430
 
BsiHKAI G_wGCw'C

Cuts at:      0      73     457     602    1070    1473 
   Size:         73     384     145     468     403

  Fragments arranged by size: 

                 468     403     384     145      73
 
Bsp1286I G_dGCh'C

Cuts at:      0      73     221     420     457     602     750    1031    1070 
   Size:         73     148     199      37     145     148     281      39

Cuts at:   1070    1115    1414    1473 
   Size:         45     299      59

  Fragments arranged by size: 

                 299     281     199     148     148     145      73      59
                  45      39      37
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     176     927     961    1473 
   Size:        176     751      34     512

  Fragments arranged by size: 

                 751     512     176      34
 
BsrFI r'CCGG_y

Cuts at:      0     680     867    1249    1473 
   Size:        680     187     382     224

  Fragments arranged by size: 

                 680     382     224     187
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     942    1473 
   Size:        942     531
 
BstBI TT'CG_AA

Cuts at:      0     843    1473 
   Size:        843     630
 
BstYI r'GATC_y

Cuts at:      0     119     762    1440    1473 
   Size:        119     643     678      33

  Fragments arranged by size: 

                 678     643     119      33
 
Bsu36I CC'TnA_GG

Cuts at:      0    1445    1473 
   Size:       1445      28
 
BtgI C'CryG_G

Cuts at:      0    1031    1473 
   Size:       1031     442
 
BtsI GCAGTG_nn'

Cuts at:      0     141    1031    1473 
   Size:        141     890     442

  Fragments arranged by size: 

                 890     442     141
 
DraIII CAC_nnn'GTG

Cuts at:      0     384    1473 
   Size:        384    1089
 
EaeI y'GGCC_r

Cuts at:      0     306     848    1251    1351    1473 
   Size:        306     542     403     100     122

  Fragments arranged by size: 

                 542     403     306     122     100
 
EarI CTCTTCn'nnn_

Cuts at:      0    1192    1473 
   Size:       1192     281
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     447     886     967    1473 
   Size:        447     439      81     506

  Fragments arranged by size: 

                 506     447     439      81
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     529     625     769    1018    1294    1473 
   Size:        529      96     144     249     276     179

  Fragments arranged by size: 

                 529     276     249     179     144      96
 
EcoO109I rG'GnC_Cy

Cuts at:      0     417     804     909    1204    1269    1326    1473 
   Size:        417     387     105     295      65      57     147

  Fragments arranged by size: 

                 417     387     295     147     105      65      57
 
HaeII r_GCGC'y

Cuts at:      0      21     250    1473 
   Size:         21     229    1223

  Fragments arranged by size: 

                1223     229      21
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     636     668    1473 
   Size:        636      32     805

  Fragments arranged by size: 

                 805     636      32
 
HincII GTy'rAC

Cuts at:      0    1101    1473 
   Size:       1101     372
 
HindIII A'AGCT_T

Cuts at:      0    1126    1473 
   Size:       1126     347
 
KasI G'GCGC_C

Cuts at:      0     246    1473 
   Size:        246    1227
 
MslI CAynn'nnrTG

Cuts at:      0     535    1228    1473 
   Size:        535     693     245

  Fragments arranged by size: 

                 693     535     245
 
MspA1I CmG'CkG

Cuts at:      0     145     311    1187    1473 
   Size:        145     166     876     286

  Fragments arranged by size: 

                 876     286     166     145
 
NaeI GCC'GGC

Cuts at:      0     682     869    1473 
   Size:        682     187     604

  Fragments arranged by size: 

                 682     604     187
 
NarI GG'CG_CC

Cuts at:      0     247    1473 
   Size:        247    1226
 
NgoMIV G'CCGG_C

Cuts at:      0     680     867    1473 
   Size:        680     187     606

  Fragments arranged by size: 

                 680     606     187
 
PasI CC'CwG_GG

Cuts at:      0     633    1424    1473 
   Size:        633     791      49

  Fragments arranged by size: 

                 791     633      49
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     384     507    1473 
   Size:        384     123     966

  Fragments arranged by size: 

                 966     384     123
 
PfoI T'CCnGG_A

Cuts at:      0     785    1473 
   Size:        785     688
 
PmlI CAC'GTG

Cuts at:      0     192    1473 
   Size:        192    1281
 
PpuMI rG'GwC_Cy

Cuts at:      0     804    1204    1269    1473 
   Size:        804     400      65     204

  Fragments arranged by size: 

                 804     400     204      65
 
PspOMI G'GGCC_C

Cuts at:      0     416    1473 
   Size:        416    1057
 
PstI C_TGCA'G

Cuts at:      0    1186    1268    1473 
   Size:       1186      82     205

  Fragments arranged by size: 

                1186     205      82
 
PvuII CAG'CTG

Cuts at:      0     145    1473 
   Size:        145    1328
 
SalI G'TCGA_C

Cuts at:      0    1099    1473 
   Size:       1099     374
 
SfcI C'TryA_G

Cuts at:      0    1182    1208    1264    1473 
   Size:       1182      26      56     209

  Fragments arranged by size: 

                1182     209      56      26
 
SfoI GGC'GCC

Cuts at:      0     248    1473 
   Size:        248    1225
 
SmaI CCC'GGG

Cuts at:      0     463    1402    1473 
   Size:        463     939      71

  Fragments arranged by size: 

                 939     463      71
 
SrfI GCCC'GGGC

Cuts at:      0     463    1473 
   Size:        463    1010
 
StuI AGG'CCT

Cuts at:      0     373    1175    1305    1473 
   Size:        373     802     130     168

  Fragments arranged by size: 

                 802     373     168     130
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     625    1182    1473 
   Size:        625     557     291

  Fragments arranged by size: 

                 625     557     291
 
TatI w'GTAC_w

Cuts at:      0     639    1473 
   Size:        639     834
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      79     111    1473 
   Size:         79      32    1362

  Fragments arranged by size: 

                1362      79      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1038    1431    1473 
   Size:       1038     393      42

  Fragments arranged by size: 

                1038     393      42
 
XmaI C'CCGG_G

Cuts at:      0     461    1400    1473 
   Size:        461     939      73

  Fragments arranged by size: 

                 939     461      73
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AleI    AlwNI     ApaI     ApoI     AvaI
    BamHI     BanI    BanII     BbeI     BbsI     BclI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BpmI   Bpu10I     BsaI    BsaAI
    BsaHI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I    BspMI
    BsrFI   BstAPI    BstBI    BstYI   Bsu36I     BtgI     BtsI   DraIII
     EaeI     EarI     EciI  Eco57MI EcoO109I    HaeII    Hin4I   HincII
  HindIII     KasI     MslI   MspA1I     NaeI     NarI   NgoMIV     PasI
    PflMI     PfoI     PmlI    PpuMI   PspOMI     PstI    PvuII     SalI
     SfcI     SfoI     SmaI     SrfI     StuI    TaqII     TatI     TstI
     XcmI     XmaI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AlfI     AloI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI
    BbvCI     BcgI    BciVI    BfrBI     BmtI     BplI    BpuEI    BsaBI
    BsaWI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI
    BsrDI    BsrGI   BssHII    BssSI   BstEII    BstXI  BstZ17I    BtgZI
     ClaI    CspCI     DraI     DrdI     EagI  EcoICRI    EcoNI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI     HpaI     KpnI     MfeI
     MluI     MmeI     MscI     NcoI     NdeI     NheI     NotI     NruI
     NsiI     NspI     PacI     PciI     PmeI     PpiI    PshAI     PsiI
    PspXI     PsrI     PvuI    RsrII     SacI    SacII    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SgfI    SgrAI     SmlI    SnaBI
     SpeI     SphI     SspI     StyI     SwaI  Tth111I     XbaI     XhoI
     XmnI     ZraI
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