Restriction map for pF1KB8112
##################################
# NO CUT ENZYMES (  174 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp718I     AspEI       AspI        
AvaI        AviII       AvrII       BalI        BamHI       
BanI        BanII       BbeI        BbrPI       BbvCI       
BcgI        BciVI       BclI        BfrI        BglI        
BglII       BlnI        BlpI        BpmI        Bpu1102I    
BsaAI       BsaBI       BsaHI       BsaI        BsaJI       
BsaWI       BseAI       BseRI       BsgI        BsiEI       
BsiWI       BsmAI       BsmBI       BsmI        BsoBI       
Bsp1407I    BspEI       BspLU11I    BspMI       BsrBI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstXI       BstZ17I     CelII       CfoI        Cfr10I      
CpoI        DraII       DraIII      DrdI        DsaI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      EcoNI       EcoO109I    EcoO65I     EcoRI       
EcoRV       EcoT14I     EcoT22I     FbaI        FseI        
FspI        HaeII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HindIII     HpaI        
HphI        KasI        KpnI        Ksp632I     KspI        
MaeIII      MamI        MfeI        MluI        MluNI       
MroI        MscI        MspA1I      MunI        MvnI        
MwoI        NaeI        NarI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
PvuII       RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
StyI        SwaI        Tsp45I      Tth111I     XbaI        
XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   54 enzymes) #
###################################

AccI          238  GTAGAC               GT^[AC][GT]AC
AcsI          221  AAATTT               [GA]^AATT[CT]
AfaI          205  GTAC                 GT^AC
AluI          417  AGCT                 AG^CT
AlwNI          56  CAGTTCCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          124  CCTCAGG              CC^T[ACGT]AGG
ApoI          221  AAATTT               [GA]^AATT[CT]
Asp700I        52  GAACCAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         338  GTGCTC               G[AT]GC[AT]^C
AvaII         284  GGTCC                G^G[AT]CC
BbsI          387  GAAGAC               GAAGAC(2/6)
BcnI            5  CCGGG                CC^[GC]GG
BmyI          338  GTGCTC               G[AGT]GC[ACT]^C
BpuAI         387  GAAGAC               GAAGAC(2/6)
BsiHKAI       338  GTGCTC               G[AT]GC[AT]^C
Bsp1286I      338  GTGCTC               G[AGT]GC[ACT]^C
BspDI         318  ATCGAT               AT^CGAT
BspHI         212  TCATGA               T^CATGA
BsrDI         114  CATTGC               GCAATG(2/0)
BsrI           55  CCAGT                ACTGG(1/-1)
BstF5I        229  CATCC                GGATG(2/0)
BstYI         299  AGATCC               [GA]^GATC[CT]
Bsu36I        124  CCTCAGG              CC^T[ACGT]AGG
ClaI          318  ATCGAT               AT^CGAT
DdeI          125  CTCAG                C^T[ACGT]AG
DraI          154  TTTAAA               TTT^AAA
EaeI            2  TGGCCG               [CT]^GGCC[GA]
Eco81I        124  CCTCAGG              CC^T[ACGT]AGG
FauI           14  CCCGC                CCCGC(4/6)
FokI          229  CATCC                GGATG(9/13)
HapII           5  CCGG                 C^CGG
HinfI         130  GATTC                G^A[ACGT]TC
HpaII           5  CCGG                 C^CGG
MaeII         207  ACGT                 A^CGT
MflI          299  AGATCC               [GA]^GATC[CT]
MslI          229  CATCCTAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI            5  CCGG                 C^CGG
NciI            5  CCGGG                CC^[GC]GG
NlaIV         284  GGTCCC               GG[ACGT]^[ACGT]CC
PflMI          55  CCAGTTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI          295  CTGCAG               CTGCA^G
RcaI          212  TCATGA               T^CATGA
RsaI          205  GTAC                 GT^AC
SfaNI          65  GCATC                GCATC(5/9)
SfcI          295  CTGCAG               C^T[GA][CT]AG
SspI          451  AATATT               AAT^ATT
StuI          326  AGGCCT               AGG^CCT
TaqI          319  TCGA                 T^CGA
TfiI          130  GATTC                G^A[AT]TC
Tsp509I       222  AATT                 ^AATT
TthHB8I       319  TCGA                 T^CGA
Van91I         55  CCAGTTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII         299  AGATCC               [GA]^GATC[CT]
XmnI           52  GAACCAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

BbvI          193  GCAGC                GCAGC(8/12)
                9  GCTGC               
BfaI          419  CTAG                 C^TAG
              431  CTAG                
BsmFI         148  GGGAC                GGGAC(10/14)
              285  GTCCC               
BstNI          61  CCTGG                CC^[AT]GG
              324  CCAGG               
Cac8I          45  GCTGGC               GC[ACGT]^[ACGT]GC
              118  GCTGGC              
Cfr13I        121  GGCCC                G^G[ACGT]CC
              284  GGTCC               
EcoRII         61  CCTGG                ^CC[AT]GG
              324  CCAGG               
Fnu4HI          9  GCTGC                GC^[ACGT]GC
              193  GCAGC               
ItaI            9  GCTGC                GC^[ACGT]GC
              193  GCAGC               
MaeI          419  CTAG                 C^TAG
              431  CTAG                
MseI          155  TTAA                 T^TAA
              334  TTAA                
MvaI           61  CCTGG                CC^[AT]GG
              324  CCAGG               
Sau96I        121  GGCCC                G^G[ACGT]CC
              284  GGTCC               
Tru9I         155  TTAA                 T^TAA
              334  TTAA                
TseI            9  GCTGC                G^C[AT]GC
              193  GCAGC               
TspRI         293  CACTG                CA[GC]TG[ACGT][ACGT]^
              382  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   14 enzymes) #
#########################################

AciI        AlwI        BsiYI       BslI        BssKI       
DpnII       HaeIII      MboI        MboII       MnlI        
NdeII       NlaIII      Sau3AI      ScrFI       
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