################################## # NO CUT ENZYMES ( 172 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaII AviII AvrII BalI BamHI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsgI BsiHKAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I Cac8I CelII Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII DsaI Eam1105I EarI Eco47III Eco81I EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HgaI Hin1I HincII HindII HpaI KasI KpnI Ksp632I KspI MaeII MamI MfeI MluI MluNI MroI MscI MspA1I MunI MwoI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuII RcaI RsrII SacI SacII SalI SapI Sau96I SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tsp45I Tth111I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AccI 226 GTCTAC GT^[AC][GT]AC AfaI 151 GTAC GT^AC AvaI 140 CCCGGG C^[CT]CG[GA]G BanI 426 GGCACC G^G[CT][GA]CC BbvI 320 GCAGC GCAGC(8/12) BfaI 239 CTAG C^TAG BglII 170 AGATCT A^GATCT BsaI 450 GAGACC GGTCTC(1/5) BsmAI 450 GAGAC GTCTC(1/5) BsmFI 343 GGGAC GGGAC(10/14) BsoBI 140 CCCGGG C^[CT]CG[GA]G BstYI 170 AGATCT [GA]^GATC[CT] DdeI 213 CTTAG C^T[ACGT]AG DrdI 360 GACCCTGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 111 CGGCCG [CT]^GGCC[GA] EagI 111 CGGCCG C^GGCCG EclXI 111 CGGCCG C^GGCCG Eco52I 111 CGGCCG C^GGCCG EcoNI 433 CCTCCTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HaeIII 112 GGCC GG^CC HapII 141 CCGG C^CGG HindIII 210 AAGCTT A^AGCTT HpaII 141 CCGG C^CGG HphI 98 TCACC GGTGA(8/7) MaeI 239 CTAG C^TAG MaeIII 19 GTTAC ^GT[ACGT]AC MflI 170 AGATCT [GA]^GATC[CT] MslI 177 CACTTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 141 CCGG C^CGG NspI 73 ACATGC [GA]CATG^[CT] PflMI 120 CCACCGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 10 GAGTC GAGTC(4/5) PstI 364 CTGCAG CTGCA^G PvuI 56 CGATCG CGAT^CG RsaI 151 GTAC GT^AC SfaNI 187 GATGC GCATC(5/9) SfcI 364 CTGCAG C^T[GA][CT]AG SmaI 140 CCCGGG CCC^GGG TfiI 352 GATTC G^A[AT]TC TseI 320 GCAGC G^C[AT]GC TspRI 80 CACTG CA[GC]TG[ACGT][ACGT]^ Van91I 120 CCACCGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 170 AGATCT [GA]^GATC[CT] XmaCI 140 CCCGGG C^CCGGG XmaI 140 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AluI 211 AGCT AG^CT 237 AGCT BbsI 69 GAAGAC GAAGAC(2/6) 94 GTCTTC BcnI 140 CCCGG CC^[GC]GG 141 CCGGG BmyI 249 GGGCAC G[AGT]GC[ACT]^C 425 GGGCAC BpuAI 69 GAAGAC GAAGAC(2/6) 94 GTCTTC BsiYI 120 CCACCGAATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 433 CCTCCTTCAGG BslI 120 CCACCGAATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 433 CCTCCTTCAGG Bsp1286I 249 GGGCAC G[AGT]GC[ACT]^C 425 GGGCAC BstF5I 182 GGATG GGATG(2/0) 186 GGATG BstNI 24 CCAGG CC^[AT]GG 295 CCTGG CfoI 15 GCGC GCG^C 444 GCGC DpnII 57 GATC ^GATC 171 GATC EcoRII 24 CCAGG ^CC[AT]GG 295 CCTGG Fnu4HI 113 GCCGC GC^[ACGT]GC 320 GCAGC FokI 182 GGATG GGATG(9/13) 186 GGATG HhaI 15 GCGC GCG^C 444 GCGC HinP1I 15 GCGC G^CGC 444 GCGC HinfI 10 GAGTC G^A[ACGT]TC 352 GATTC ItaI 113 GCCGC GC^[ACGT]GC 320 GCAGC MboI 57 GATC ^GATC 171 GATC MboII 69 GAAGA GAAGA(8/7) 95 TCTTC MvaI 24 CCAGG CC^[AT]GG 295 CCTGG NciI 140 CCCGG CC^[GC]GG 141 CCGGG NdeII 57 GATC ^GATC 171 GATC NlaIV 42 GGAGCC GG[ACGT]^[ACGT]CC 426 GGCACC Sau3AI 57 GATC ^GATC 171 GATC TaqI 59 TCGA T^CGA 303 TCGA TthHB8I 59 TCGA T^CGA 303 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 13 enzymes) # ######################################### AccII AciI BsaJI BsiEI BssKI BstUI MnlI MseI MvnI NlaIII ScrFI Tru9I Tsp509I