Restriction map for pF1KB8105
##################################
# NO CUT ENZYMES (  175 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AciI        
AclI        AcsI        AflII       AflIII      AgeI        
AhdI        AluI        Alw44I      AlwNI       Aor51HI     
ApaI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspHI       
AvaI        AviII       AvrII       BamHI       BanII       
BbeI        BbrPI       BbvCI       BbvI        BcgI        
BciVI       BclI        BcnI        BfrI        BglI        
BglII       BlnI        BlpI        BpmI        Bpu1102I    
BsaAI       BsaBI       BseRI       BsgI        BsiEI       
BsiHKAI     BsiWI       BsmBI       BsmI        BsoBI       
Bsp1407I    BspDI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstXI       BstZ17I     Cac8I       CelII       
CfoI        Cfr10I      ClaI        CpoI        DraI        
DraIII      DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoNI       EcoO65I     EcoRI       
EcoRV       EcoT22I     FauI        FbaI        Fnu4HI      
FseI        FspI        HaeII       HhaI        HinP1I      
HindIII     HpaI        HphI        ItaI        KasI        
KpnI        KspI        MaeII       MaeIII      MamI        
MfeI        MluI        MslI        MspA1I      MunI        
MvnI        NaeI        NarI        NciI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PstI        PvuI        
PvuII       RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfaNI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        TaqI        
TseI        Tsp45I      Tsp509I     TthHB8I     Van91I      
XbaI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   55 enzymes) #
###################################

AcyI          220  GACGCC               G[GA]^CG[CT]C
AlwI           60  GATCC                GGATC(4/5)
AocI          214  CCTGAGG              CC^T[ACGT]AGG
AspI           79  GACAGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI          171  TGGCCA               TGG^CCA
BanI          120  GGTGCC               G^G[CT][GA]CC
BbsI          301  GAAGAC               GAAGAC(2/6)
BfaI          148  CTAG                 C^TAG
BmyI          151  GTGCCC               G[AGT]GC[ACT]^C
BpuAI         301  GAAGAC               GAAGAC(2/6)
BsaHI         220  GACGCC               G[GA]^CG[CT]C
BsaI          210  GAGACC               GGTCTC(1/5)
BsiYI           9  CCAGTACAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI            9  CCAGTACAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI         210  GAGAC                GTCTC(1/5)
BsmFI          85  GTCCC                GGGAC(10/14)
Bsp1286I      151  GTGCCC               G[AGT]GC[ACT]^C
BspHI         294  TCATGA               T^CATGA
BssKI         116  CCAGG                ^CC[ACGT]GG
BstNI         116  CCAGG                CC^[AT]GG
BstYI          59  AGATCC               [GA]^GATC[CT]
Bsu36I        214  CCTGAGG              CC^T[ACGT]AGG
DraII          83  GGGTCCC              [GA]G^G[ACGT]CC[CT]
DsaI          269  CCATGG               C^C[GA][CT]GG
EaeI          171  TGGCCA               [CT]^GGCC[GA]
EarI          195  GAAGAG               CTCTTC(1/4)
Eco81I        214  CCTGAGG              CC^T[ACGT]AGG
EcoO109I       83  GGGTCCC              [GA]G^G[ACGT]CC[CT]
EcoRII        116  CCAGG                ^CC[AT]GG
EcoT14I       269  CCATGG               C^C[AT][AT]GG
HgaI          220  GACGC                GACGC(5/10)
Hin1I         220  GACGCC               G[GA]^CG[CT]C
HincII        238  GTCAAC               GT[CT]^[GA]AC
HindII        238  GTCAAC               GT[CT]^[GA]AC
HinfI         200  GAATC                G^A[ACGT]TC
Ksp632I       195  GAAGAG               CTCTTC(1/4)
MaeI          148  CTAG                 C^TAG
MflI           59  AGATCC               [GA]^GATC[CT]
MluNI         171  TGGCCA               TGG^CCA
MscI          171  TGGCCA               TGG^CCA
MseI          187  TTAA                 T^TAA
MvaI          116  CCAGG                CC^[AT]GG
MwoI          106  GCTCCAAAAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI          269  CCATGG               C^CATGG
PflFI          79  GACAGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI          83  GGGTCCC              [GA]G^G[AT]CC[CT]
RcaI          294  TCATGA               T^CATGA
ScrFI         116  CCAGG                CC^[ACGT]GG
SfcI          324  CTACAG               C^T[GA][CT]AG
StyI          269  CCATGG               C^C[AT][AT]GG
TfiI          200  GAATC                G^A[AT]TC
Tru9I         187  TTAA                 T^TAA
Tth111I        79  GACAGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI          260  CCAGTGCTACCATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII          59  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   13 enzymes) #
###################################

AfaI           12  GTAC                 GT^AC
              144  GTAC                
AvaII          21  GGACC                G^G[AT]CC
               84  GGTCC               
BsaJI         116  CCAGGG               C^C[ACGT][ACGT]GG
              269  CCATGG              
BstF5I         25  CATCC                GGATG(2/0)
              249  CATCC               
DdeI          208  CTGAG                C^T[ACGT]AG
              215  CTGAG               
DpnII          60  GATC                 ^GATC
              127  GATC                
FokI           25  CATCC                GGATG(9/13)
              249  CATCC               
MboI           60  GATC                 ^GATC
              127  GATC                
NdeII          60  GATC                 ^GATC
              127  GATC                
NlaIII        270  CATG                 CATG^
              295  CATG                
RsaI           12  GTAC                 GT^AC
              144  GTAC                
Sau3AI         60  GATC                 ^GATC
              127  GATC                
TspRI         261  CAGTG                CA[GC]TG[ACGT][ACGT]^
              327  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   15 enzymes) #
#########################################

AccIII      BsaWI       BseAI       BspEI       BsrI        
Cfr13I      HaeIII      HapII       HpaII       MboII       
MnlI        MroI        MspI        NlaIV       Sau96I      
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