Restriction map for pF1KB8101
##################################
# NO CUT ENZYMES (  125 enzymes) #
##################################

Acc65I      AccII       AccIII      AclI        AflII       
AhdI        Alw44I      AlwI        AocI        Aor51HI     
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AvaI        AviII       
AvrII       BamHI       BanI        BbeI        BbrPI       
BbsI        BbvCI       BcgI        BclI        BfaI        
BfrI        BglI        BlnI        BpuAI       BsaBI       
BseAI       BsiEI       BsiWI       BsmBI       BsoBI       
BspEI       BspHI       BspLU11I    BsrDI       BssHII      
BssSI       BstAPI      BstBI       BstEII      BstPI       
BstUI       Bsu36I      DraI        DrdI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO65I     EcoRI       EcoRV       
FbaI        FseI        FspI        HindIII     HpaI        
KasI        KpnI        Ksp632I     KspI        MaeI        
MamI        MfeI        MluI        MroI        MseI        
MunI        MvnI        NarI        NcoI        NdeI        
NheI        NotI        NruI        NspV        PacI        
PaeR7I      PflFI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        RcaI        SacII       SalI        SapI        
SbfI        ScaI        SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
Sse8387I    SspI        SwaI        Tru9I       Tth111I     
XbaI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AcsI          235  AAATTC               [GA]^AATT[CT]
AflIII        998  ACGTGT               A^C[GA][CT]GT
AgeI           34  ACCGGT               A^CCGGT
AlwNI         169  CAGTTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI          181  GGGCCC               GGGCC^C
ApoI          235  AAATTC               [GA]^AATT[CT]
AspHI         126  GAGCTC               G[AT]GC[AT]^C
BciVI         905  GTATCC               GTATCC(6/5)
BglII         344  AGATCT               A^GATCT
BlpI         1333  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     1333  GCTGAGC              GC^T[ACGT]AGC
BsaAI         997  TACGTG               [CT]AC^GT[GA]
BsgI           91  CTGCAC               GTGCAG(16/14)
BsiHKAI       126  GAGCTC               G[AT]GC[AT]^C
BsmI           46  GAATGC               GAATGC(1/-1)
Bsp1407I      632  TGTACA               T^GTACA
BspDI         544  ATCGAT               AT^CGAT
BspMI        1237  ACCTGC               ACCTGC(4/8)
BsrBI         679  CCGCTC               CCGCTC(-3/-3)
BsrGI         632  TGTACA               T^GTACA
Bst1107I      276  GTATAC               GTA^TAC
BstXI         386  CCAATCTGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         344  AGATCT               [GA]^GATC[CT]
BstZ17I       276  GTATAC               GTA^TAC
CelII        1333  GCTGAGC              GC^T[ACGT]AGC
ClaI          544  ATCGAT               AT^CGAT
CpoI          863  CGGACCG              CG^G[AT]CCG
DraIII       1021  CACAACGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI         1076  CCACGG               C^C[GA][CT]GG
EcoT14I       377  CCTTGG               C^C[AT][AT]GG
EcoT22I        48  ATGCAT               ATGCA^T
HgaI          937  GACGC                GACGC(5/10)
MboII          81  GAAGA                GAAGA(8/7)
MflI          344  AGATCT               [GA]^GATC[CT]
NaeI         1101  GCCGGC               GCC^GGC
NgoMIV       1101  GCCGGC               G^CCGGC
NsiI           48  ATGCAT               ATGCA^T
PflMI         558  CCATGCCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI          34  ACCGGT               A^CCGGT
RsrII         863  CGGACCG              CG^G[AT]CCG
SacI          126  GAGCTC               GAGCT^C
SexAI         971  ACCAGGT              A^CC[AT]GGT
SmlI          607  CTTGAG               C^T[CT][GA]AG
SphI          500  GCATGC               GCATG^C
SspBI         632  TGTACA               T^GTACA
StuI          374  AGGCCT               AGG^CCT
StyI          377  CCTTGG               C^C[AT][AT]GG
TfiI          228  GATTC                G^A[AT]TC
Tsp509I       236  AATT                 ^AATT
Van91I        558  CCATGCCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII         344  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AatII         508  GACGTC               GACGT^C
             1318  GACGTC              
AfaI          633  GTAC                 GT^AC
             1044  GTAC                
BalI          555  TGGCCA               TGG^CCA
              882  TGGCCA              
BcnI          650  CCCGG                CC^[GC]GG
              861  CCCGG               
BseRI         129  CTCCTC               GAGGAG(10/8)
              148  GAGGAG              
BsrFI          34  ACCGGT               [GA]^CCGG[CT]
             1101  GCCGGC              
BsrI          212  ACTGG                ACTGG(1/-1)
              553  ACTGG               
Cfr10I         34  ACCGGT               [GA]^CCGG[CT]
             1101  GCCGGC              
DraII         180  GGGGCCC              [GA]G^G[ACGT]CC[CT]
              181  GGGCCCC             
EaeI          555  TGGCCA               [CT]^GGCC[GA]
              882  TGGCCA              
EcoO109I      180  GGGGCCC              [GA]G^G[ACGT]CC[CT]
              181  GGGCCCC             
FauI          678  CCCGC                CCCGC(4/6)
              824  CCCGC               
HaeII         413  GGCGCT               [GA]GCGC^[CT]
              567  GGCGCT              
HphI          285  GGTGA                GGTGA(8/7)
               41  TCACC               
MluNI         555  TGGCCA               TGG^CCA
              882  TGGCCA              
MscI          555  TGGCCA               TGG^CCA
              882  TGGCCA              
NciI          650  CCCGG                CC^[GC]GG
              861  CCCGG               
NspI          398  GCATGT               [GA]CATG^[CT]
              500  GCATGC              
PvuII         256  CAGCTG               CAG^CTG
              835  CAGCTG              
RsaI          633  GTAC                 GT^AC
             1044  GTAC                


#########################################
# 3 OR MORE CUT ENZYMES (   62 enzymes) #
#########################################

AccI        AciI        AcyI        AluI        AvaII       
BanII       BbvI        BmyI        BpmI        BsaHI       
BsaI        BsaJI       BsaWI       BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BssKI       BstF5I      
BstNI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       EcoRII      Fnu4HI      FokI        HaeIII      
HapII       HhaI        Hin1I       HinP1I      HincII      
HindII      HinfI       HpaII       ItaI        MaeII       
MaeIII      MboI        MnlI        MslI        MspA1I      
MspI        MvaI        MwoI        NdeII       NlaIII      
NlaIV       PleI        Sau3AI      Sau96I      ScrFI       
SfaNI       TaqI        TseI        Tsp45I      TspRI       
TthHB8I     XcmI        
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