Restriction map for pF1KB8101
 (Linear) (Six-base) MAPSORT of: KIBB8101.seq  Check: 4412  from: 1  to: 1341



KIBB8101.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:02  ..

AatII G_ACGT'C

Cuts at:      0     512    1322    1341 
   Size:        512     810      19

  Fragments arranged by size: 

                 810     512      19
 
AccI GT'mk_AC

Cuts at:      0     277     329     512    1341 
   Size:        277      52     183     829

  Fragments arranged by size: 

                 829     277     183      52
 
AflIII A'CryG_T

Cuts at:      0     998    1341 
   Size:        998     343
 
AgeI A'CCGG_T

Cuts at:      0      34    1341 
   Size:         34    1307
 
AleI CACnn'nnGTG

Cuts at:      0    1066    1341 
   Size:       1066     275
 
AlwNI CAG_nnn'CTG

Cuts at:      0     174    1341 
   Size:        174    1167
 
ApaI G_GGCC'C

Cuts at:      0     185    1341 
   Size:        185    1156
 
ApoI r'AATT_y

Cuts at:      0     235    1341 
   Size:        235    1106
 
BanII G_rGCy'C

Cuts at:      0     130     185     667    1124    1166    1341 
   Size:        130      55     482     457      42     175

  Fragments arranged by size: 

                 482     457     175     130      55      42
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     916    1341 
   Size:        916     425
 
BfrBI ATG'CAT

Cuts at:      0      50    1341 
   Size:         50    1291
 
BglII A'GATC_T

Cuts at:      0     344    1341 
   Size:        344     997
 
BlpI GC'TnA_GC

Cuts at:      0    1334    1341 
   Size:       1334       7
 
Bme1580I G_kGCm'C

Cuts at:      0     185    1341 
   Size:        185    1156
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     147    1341 
   Size:        147    1194
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     166     196    1129    1341 
   Size:        166      30     933     212

  Fragments arranged by size: 

                 933     212     166      30
 
Bpu10I CC'TnA_GC

Cuts at:      0     910    1341 
   Size:        910     431
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     628    1341 
   Size:        628     713
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      43     604    1066    1341 
   Size:         43     561     462     275

  Fragments arranged by size: 

                 561     462     275      43
 
BsaAI yAC'GTr

Cuts at:      0     999    1341 
   Size:        999     342
 
BsaHI Gr'CG_yC

Cuts at:      0     509     938    1319    1341 
   Size:        509     429     381      22

  Fragments arranged by size: 

                 509     429     381      22
 
BsaWI w'CCGG_w

Cuts at:      0      34     101     515    1341 
   Size:         34      67     414     826

  Fragments arranged by size: 

                 826     414      67      34
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     139     169     928     958    1341 
   Size:        139      30     759      30     383

  Fragments arranged by size: 

                 759     383     139      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     120     163    1341 
   Size:        120      43    1178

  Fragments arranged by size: 

                1178     120      43
 
BseYI C'CCAG_C

Cuts at:      0      27     850    1341 
   Size:         27     823     491

  Fragments arranged by size: 

                 823     491      27
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      76    1341 
   Size:         76    1265
 
BsiHKAI G_wGCw'C

Cuts at:      0     130    1341 
   Size:        130    1211
 
BsmI GAATG_Cn'

Cuts at:      0      52    1341 
   Size:         52    1289
 
Bsp1286I G_dGCh'C

Cuts at:      0     130     185     667    1124    1166    1341 
   Size:        130      55     482     457      42     175

  Fragments arranged by size: 

                 482     457     175     130      55      42
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1246    1341 
   Size:       1246      95
 
BsrBI CCG'CTC

Cuts at:      0     681    1341 
   Size:        681     660
 
BsrFI r'CCGG_y

Cuts at:      0      34    1101    1341 
   Size:         34    1067     240

  Fragments arranged by size: 

                1067     240      34
 
BsrGI T'GTAC_A

Cuts at:      0     632    1341 
   Size:        632     709
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     393    1341 
   Size:        393     948
 
BstYI r'GATC_y

Cuts at:      0     344    1341 
   Size:        344     997
 
BstZ17I GTA'TAC

Cuts at:      0     278    1341 
   Size:        278    1063
 
BtgI C'CryG_G

Cuts at:      0    1076    1341 
   Size:       1076     265
 
ClaI AT'CG_AT

Cuts at:      0     545    1341 
   Size:        545     796
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1209    1244    1341 
   Size:       1209      35      97

  Fragments arranged by size: 

                1209      97      35
 
DraIII CAC_nnn'GTG

Cuts at:      0    1026    1341 
   Size:       1026     315
 
EaeI y'GGCC_r

Cuts at:      0     555     882    1341 
   Size:        555     327     459

  Fragments arranged by size: 

                 555     459     327
 
EcoICRI GAG'CTC

Cuts at:      0     128    1341 
   Size:        128    1213
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     166     196    1129    1341 
   Size:        166      30     933     212

  Fragments arranged by size: 

                 933     212     166      30
 
EcoO109I rG'GnC_Cy

Cuts at:      0     181     182    1341 
   Size:        181       1    1159

  Fragments arranged by size: 

                1159     181       1
 
HaeII r_GCGC'y

Cuts at:      0     417     571    1341 
   Size:        417     154     770

  Fragments arranged by size: 

                 770     417     154
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     928     960    1341 
   Size:        928      32     381

  Fragments arranged by size: 

                 928     381      32
 
HincII GTy'rAC

Cuts at:      0     222     483     618     978    1341 
   Size:        222     261     135     360     363

  Fragments arranged by size: 

                 363     360     261     222     135
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1097    1341 
   Size:       1097     244
 
MscI TGG'CCA

Cuts at:      0     557     884    1341 
   Size:        557     327     457

  Fragments arranged by size: 

                 557     457     327
 
MslI CAynn'nnrTG

Cuts at:      0      70     775     997    1066    1341 
   Size:         70     705     222      69     275

  Fragments arranged by size: 

                 705     275     222      70      69
 
MspA1I CmG'CkG

Cuts at:      0     258     827     837    1341 
   Size:        258     569      10     504

  Fragments arranged by size: 

                 569     504     258      10
 
NaeI GCC'GGC

Cuts at:      0    1103    1341 
   Size:       1103     238
 
NgoMIV G'CCGG_C

Cuts at:      0    1101    1341 
   Size:       1101     240
 
NsiI A_TGCA'T

Cuts at:      0      52    1341 
   Size:         52    1289
 
NspI r_CATG'y

Cuts at:      0     402     504    1341 
   Size:        402     102     837

  Fragments arranged by size: 

                 837     402     102
 
PasI CC'CwG_GG

Cuts at:      0     705    1035    1341 
   Size:        705     330     306

  Fragments arranged by size: 

                 705     330     306
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     564    1341 
   Size:        564     777
 
PfoI T'CCnGG_A

Cuts at:      0     860    1341 
   Size:        860     481
 
PspOMI G'GGCC_C

Cuts at:      0     181    1341 
   Size:        181    1160
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0      97     129    1341 
   Size:         97      32    1212

  Fragments arranged by size: 

                1212      97      32
 
PvuII CAG'CTG

Cuts at:      0     258     837    1341 
   Size:        258     579     504

  Fragments arranged by size: 

                 579     504     258
 
RsrII CG'GwC_CG

Cuts at:      0     864    1341 
   Size:        864     477
 
SacI G_AGCT'C

Cuts at:      0     130    1341 
   Size:        130    1211
 
SexAI A'CCwGG_T

Cuts at:      0     971    1341 
   Size:        971     370
 
SmlI C'TyrA_G

Cuts at:      0     607    1341 
   Size:        607     734
 
SphI G_CATG'C

Cuts at:      0     504    1341 
   Size:        504     837
 
StuI AGG'CCT

Cuts at:      0     376    1341 
   Size:        376     965
 
StyI C'CwwG_G

Cuts at:      0     377    1341 
   Size:        377     964
 
TatI w'GTAC_w

Cuts at:      0     632    1043    1341 
   Size:        632     411     298

  Fragments arranged by size: 

                 632     411     298
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     141     240    1065    1341 
   Size:        141      99     825     276

  Fragments arranged by size: 

                 825     276     141      99
 
ZraI GAC'GTC

Cuts at:      0     510    1320    1341 
   Size:        510     810      21

  Fragments arranged by size: 

                 810     510      21
 

 Enzymes that do cut:

    AatII     AccI   AflIII     AgeI     AleI    AlwNI     ApaI     ApoI
    BanII    BciVI    BfrBI    BglII     BlpI Bme1580I     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I    BspMI    BsrBI    BsrFI
    BsrGI    BstXI    BstYI  BstZ17I     BtgI     ClaI    CspCI   DraIII
     EaeI  EcoICRI  Eco57MI EcoO109I    HaeII    Hin4I   HincII     MmeI
     MscI     MslI   MspA1I     NaeI   NgoMIV     NsiI     NspI     PasI
    PflMI     PfoI   PspOMI     PsrI    PvuII    RsrII     SacI    SexAI
     SmlI     SphI     StuI     StyI     TatI     XcmI     ZraI

 Enzymes that do not cut: 

     AarI   Acc65I     AclI     AcuI     AfeI    AflII     AhdI     AlfI
     AloI    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
    BamHI     BanI     BbeI     BbsI    BbvCI     BcgI     BclI     BglI
    BmgBI     BmrI     BmtI    BsaBI    BsiEI    BsiWI    BsmBI    BspEI
    BspHI    BsrDI   BssHII    BssSI   BstAPI    BstBI   BstEII   Bsu36I
    BtgZI     BtsI     DraI     DrdI     EagI     EarI     EciI    EcoNI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     NarI     NcoI     NdeI     NheI
     NotI     NruI     PacI     PciI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI    PspXI     PstI     PvuI    SacII     SalI    SanDI
     SapI     SbfI     ScaI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SrfI     SspI     SwaI    TaqII     TstI
  Tth111I     XbaI     XhoI     XmaI     XmnI
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