Restriction map for pF1KB7756
##################################
# NO CUT ENZYMES (  141 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaLI       AscI        AseI        
AsnI        Asp718I     AspEI       AspI        AvrII       
BalI        BbeI        BbrPI       BbvCI       BcgI        
BclI        BfrI        BglI        BglII       BlnI        
BsaAI       BsaBI       BsaHI       BseAI       BsiEI       
BsiWI       BsmBI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrBI       BsrDI       BsrFI       
BsrGI       BssHII      Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstXI       BstZ17I     Bsu36I      
Cfr10I      ClaI        CpoI        DraI        DraIII      
DrdI        EaeI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoO65I     EcoRV       
EcoT14I     FauI        FbaI        FseI        HaeII       
HgaI        Hin1I       HincII      HindII      HpaI        
KasI        KpnI        KspI        MaeII       MamI        
MfeI        MluI        MluNI       MroI        MscI        
MspA1I      MunI        MwoI        NaeI        NarI        
NcoI        NgoMIV      NheI        NotI        NruI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
PvuII       RcaI        RsrII       SacII       SalI        
SbfI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StyI        SwaI        TaqI        
Tth111I     TthHB8I     Van91I      XbaI        XcmI        
XhoI        


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# ONE CUT ENZYMES (   38 enzymes) #
###################################

AccI          168  GTCTAC               GT^[AC][GT]AC
AccII        1092  CGCG                 CG^CG
ApaI           97  GGGCCC               GGGCC^C
AvaI          101  CCCGGG               C^[CT]CG[GA]G
AvaII         428  GGACC                G^G[AT]CC
AviII        1033  TGCGCA               TGC^GCA
BamHI         106  GGATCC               G^GATCC
BanI          158  GGTGCC               G^G[CT][GA]CC
BbsI          304  GAAGAC               GAAGAC(2/6)
BciVI         197  GGATAC               GTATCC(6/5)
BfaI          229  CTAG                 C^TAG
BpuAI         304  GAAGAC               GAAGAC(2/6)
BseRI          85  CTCCTC               GAGGAG(10/8)
BsoBI         101  CCCGGG               C^[CT]CG[GA]G
BspMI         308  ACCTGC               ACCTGC(4/8)
BssSI         163  CTCGTG               CACGAG(-5/-1)
BstUI        1092  CGCG                 CG^CG
BstYI         106  GGATCC               [GA]^GATC[CT]
Cac8I         360  GCAAGC               GC[ACGT]^[ACGT]GC
EcoNI          22  CCTTCCCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI         890  GAATTC               G^AATTC
EcoT22I       578  ATGCAT               ATGCA^T
FspI         1033  TGCGCA               TGC^GCA
HindIII       374  AAGCTT               A^AGCTT
MaeI          229  CTAG                 C^TAG
MflI          106  GGATCC               [GA]^GATC[CT]
MseI         1361  TTAA                 T^TAA
MvnI         1092  CGCG                 CG^CG
NdeI          575  CATATG               CA^TATG
NsiI          578  ATGCAT               ATGCA^T
NspI          580  GCATGT               [GA]CATG^[CT]
ScaI           15  AGTACT               AGT^ACT
SmaI          101  CCCGGG               CCC^GGG
SmlI          439  CTTGAG               C^T[CT][GA]AG
Tru9I        1361  TTAA                 T^TAA
XhoII         106  GGATCC               [GA]^GATC[CT]
XmaCI         101  CCCGGG               C^CCGGG
XmaI          101  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AciI          475  CCGC                 CCGC(-3/-1)
             1091  CCGC                
AcsI          890  GAATTC               [GA]^AATT[CT]
             1270  AAATTC              
AfaI           16  GTAC                 GT^AC
             1202  GTAC                
ApoI          890  GAATTC               [GA]^AATT[CT]
             1270  AAATTC              
BlpI          866  GCTCAGC              GC^T[ACGT]AGC
             1286  GCTCAGC             
Bpu1102I      866  GCTCAGC              GC^T[ACGT]AGC
             1286  GCTCAGC             
BstF5I       1373  CATCC                GGATG(2/0)
              290  GGATG               
CelII         866  GCTCAGC              GC^T[ACGT]AGC
             1286  GCTCAGC             
CfoI          284  GCGC                 GCG^C
             1034  GCGC                
DpnII         107  GATC                 ^GATC
              347  GATC                
DraII          30  AGGCCCT              [GA]G^G[ACGT]CC[CT]
               97  GGGCCCC             
EcoO109I       30  AGGCCCT              [GA]G^G[ACGT]CC[CT]
               97  GGGCCCC             
FokI         1373  CATCC                GGATG(9/13)
              290  GGATG               
HhaI          284  GCGC                 GCG^C
             1034  GCGC                
HinP1I        284  GCGC                 G^CGC
             1034  GCGC                
HphI          349  TCACC                GGTGA(8/7)
              791  TCACC               
MboI          107  GATC                 ^GATC
              347  GATC                
NdeII         107  GATC                 ^GATC
              347  GATC                
RsaI           16  GTAC                 GT^AC
             1202  GTAC                
Sau3AI        107  GATC                 ^GATC
              347  GATC                
SfcI          828  CTACAG               C^T[GA][CT]AG
             1080  CTACAG              
Tsp509I       891  AATT                 ^AATT
             1271  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (   57 enzymes) #
#########################################

AluI        AlwI        AlwNI       Asp700I     AspHI       
BanII       BbvI        BcnI        BmyI        BpmI        
BsaI        BsaJI       BsaWI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsmI        
Bsp1286I    BsrI        BssKI       BstNI       Cfr13I      
DdeI        DsaI        EarI        EcoRII      Fnu4HI      
HaeIII      HapII       HinfI       HpaII       ItaI        
Ksp632I     MaeIII      MboII       MnlI        MslI        
MspI        MvaI        NciI        NlaIII      NlaIV       
PleI        SacI        SapI        Sau96I      ScrFI       
SfaNI       StuI        TfiI        TseI        Tsp45I      
TspRI       XmnI        
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