Restriction map for pF1KB7756
 (Linear) (Six-base) MAPSORT of: KIBB7756.seq  Check: 9227  from: 1  to: 1494



KIBB7756.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:02  ..

AccI GT'mk_AC

Cuts at:      0     169    1494 
   Size:        169    1325
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     102     392     588     672     756     840    1008    1092 
   Size:        102     290     196      84      84      84     168      84

Cuts at:   1092    1176    1260    1494 
   Size:         84      84     234

  Fragments arranged by size: 

                 290     234     196     168     102      84      84      84
                  84      84      84
 
AleI CACnn'nnGTG

Cuts at:      0    1405    1494 
   Size:       1405      89
 
AlwNI CAG_nnn'CTG

Cuts at:      0      34     611     863     926    1031    1494 
   Size:         34     577     252      63     105     463

  Fragments arranged by size: 

                 577     463     252     105      63      34
 
ApaI G_GGCC'C

Cuts at:      0     101    1494 
   Size:        101    1393
 
ApoI r'AATT_y

Cuts at:      0     890    1270    1494 
   Size:        890     380     224

  Fragments arranged by size: 

                 890     380     224
 
AvaI C'yCGr_G

Cuts at:      0     101    1494 
   Size:        101    1393
 
BamHI G'GATC_C

Cuts at:      0     106    1494 
   Size:        106    1388
 
BanI G'GyrC_C

Cuts at:      0     158    1494 
   Size:        158    1336
 
BanII G_rGCy'C

Cuts at:      0     101     700    1288    1372    1494 
   Size:        101     599     588      84     122

  Fragments arranged by size: 

                 599     588     122     101      84
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     311    1494 
   Size:        311    1183
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     191    1494 
   Size:        191    1303
 
BfrBI ATG'CAT

Cuts at:      0     580    1494 
   Size:        580     914
 
BlpI GC'TnA_GC

Cuts at:      0     867    1287    1494 
   Size:        867     420     207

  Fragments arranged by size: 

                 867     420     207
 
Bme1580I G_kGCm'C

Cuts at:      0     101     491    1494 
   Size:        101     390    1003

  Fragments arranged by size: 

                1003     390     101
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     258    1160    1494 
   Size:        258     902     334

  Fragments arranged by size: 

                 902     334     258
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     719    1494 
   Size:        719     775
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      25     308     316     668     752    1494 
   Size:         25     283       8     352      84     742

  Fragments arranged by size: 

                 742     352     283      84      25       8
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     460    1494 
   Size:        460    1034
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      88     453     495    1494 
   Size:         88     365      42     999

  Fragments arranged by size: 

                 999     365      88      42
 
BsaWI w'CCGG_w

Cuts at:      0     814     898    1318    1445    1494 
   Size:        814      84     420     127      49

  Fragments arranged by size: 

                 814     420     127      84      49
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      76    1494 
   Size:         76    1418
 
BseYI C'CCAG_C

Cuts at:      0      94     245    1494 
   Size:         94     151    1249

  Fragments arranged by size: 

                1249     151      94
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     687     771    1023    1191    1470    1494 
   Size:        687      84     252     168     279      24

  Fragments arranged by size: 

                 687     279     252     168      84      24
 
BsiHKAI G_wGCw'C

Cuts at:      0     700    1288    1372    1494 
   Size:        700     588      84     122

  Fragments arranged by size: 

                 700     588     122      84
 
BsmI GAATG_Cn'

Cuts at:      0     786     922    1426    1494 
   Size:        786     136     504      68

  Fragments arranged by size: 

                 786     504     136      68
 
Bsp1286I G_dGCh'C

Cuts at:      0     101     491     700    1288    1372    1494 
   Size:        101     390     209     588      84     122

  Fragments arranged by size: 

                 588     390     209     122     101      84
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     317    1494 
   Size:        317    1177
 
BssSI C'ACGA_G

Cuts at:      0     163    1494 
   Size:        163    1331
 
BstYI r'GATC_y

Cuts at:      0     106    1494 
   Size:        106    1388
 
BtgI C'CryG_G

Cuts at:      0     175     423     491    1494 
   Size:        175     248      68    1003

  Fragments arranged by size: 

                1003     248     175      68
 
BtsI GCAGTG_nn'

Cuts at:      0     129     675     759    1494 
   Size:        129     546      84     735

  Fragments arranged by size: 

                 735     546     129      84
 
EarI CTCTTCn'nnn_

Cuts at:      0     439     631     759    1494 
   Size:        439     192     128     735

  Fragments arranged by size: 

                 735     439     192     128
 
EcoICRI GAG'CTC

Cuts at:      0     698    1286    1370    1494 
   Size:        698     588      84     124

  Fragments arranged by size: 

                 698     588     124      84
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      25     102     308     316     392     588     668     672 
   Size:         25      77     206       8      76     196      80       4

Cuts at:    672     752     756     840    1008    1092    1176    1260    1494 
   Size:         80       4      84     168      84      84      84     234

  Fragments arranged by size: 

                 234     206     196     168      84      84      84      84
                  80      80      77      76      25       8       4       4
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      26    1494 
   Size:         26    1468
 
EcoO109I rG'GnC_Cy

Cuts at:      0      31      98    1494 
   Size:         31      67    1396

  Fragments arranged by size: 

                1396      67      31
 
EcoRI G'AATT_C

Cuts at:      0     890    1494 
   Size:        890     604
 
FspI TGC'GCA

Cuts at:      0    1035    1494 
   Size:       1035     459
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     438     452     470     484    1191    1223    1494 
   Size:        438      14      18      14     707      32     271

  Fragments arranged by size: 

                 707     438     271      32      18      14      14
 
HindIII A'AGCT_T

Cuts at:      0     374    1494 
   Size:        374    1120
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     248    1494 
   Size:        248    1246
 
MslI CAynn'nnrTG

Cuts at:      0     579    1251    1405    1494 
   Size:        579     672     154      89

  Fragments arranged by size: 

                 672     579     154      89
 
NdeI CA'TA_TG

Cuts at:      0     576    1494 
   Size:        576     918
 
NsiI A_TGCA'T

Cuts at:      0     582    1494 
   Size:        582     912
 
NspI r_CATG'y

Cuts at:      0     584    1494 
   Size:        584     910
 
PasI CC'CwG_GG

Cuts at:      0     409    1494 
   Size:        409    1085
 
PfoI T'CCnGG_A

Cuts at:      0     211    1494 
   Size:        211    1283
 
PspOMI G'GGCC_C

Cuts at:      0      97    1494 
   Size:         97    1397
 
SacI G_AGCT'C

Cuts at:      0     700    1288    1372    1494 
   Size:        700     588      84     122

  Fragments arranged by size: 

                 700     588     122      84
 
SapI GCTCTTCn'nnn_

Cuts at:      0     439     631     759    1494 
   Size:        439     192     128     735

  Fragments arranged by size: 

                 735     439     192     128
 
ScaI AGT'ACT

Cuts at:      0      17    1494 
   Size:         17    1477
 
SfcI C'TryA_G

Cuts at:      0     828    1080    1494 
   Size:        828     252     414

  Fragments arranged by size: 

                 828     414     252
 
SmaI CCC'GGG

Cuts at:      0     103    1494 
   Size:        103    1391
 
SmlI C'TyrA_G

Cuts at:      0     439    1494 
   Size:        439    1055
 
StuI AGG'CCT

Cuts at:      0     685     937    1021    1105    1189    1441    1494 
   Size:        685     252      84      84      84     252      53

  Fragments arranged by size: 

                 685     252     252      84      84      84      53
 
TatI w'GTAC_w

Cuts at:      0      15    1494 
   Size:         15    1479
 
XmaI C'CCGG_G

Cuts at:      0     101    1494 
   Size:        101    1393
 
XmnI GAAnn'nnTTC

Cuts at:      0     448     685     853     937    1021    1105    1189    1273 
   Size:        448     237     168      84      84      84      84      84

Cuts at:   1273    1441    1494 
   Size:        168      53

  Fragments arranged by size: 

                 448     237     168     168      84      84      84      84
                  84      53
 

 Enzymes that do cut:

     AccI     AcuI     AleI    AlwNI     ApaI     ApoI     AvaI    BamHI
     BanI    BanII     BbsI    BciVI    BfrBI     BlpI Bme1580I     BmrI
     BplI     BpmI    BpuEI     BsaI    BsaWI    BseRI    BseYI     BsgI
  BsiHKAI     BsmI Bsp1286I    BspMI    BssSI    BstYI     BtgI     BtsI
     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FspI    Hin4I
  HindIII     MmeI     MslI     NdeI     NsiI     NspI     PasI     PfoI
   PspOMI     SacI     SapI     ScaI     SfcI     SmaI     SmlI     StuI
     TatI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AlfI     AloI    ApaLI     AscI     AseI    AsiSI    AvrII
     BaeI     BbeI    BbvCI     BcgI     BclI     BglI    BglII    BmgBI
     BmtI   Bpu10I    BsaAI    BsaBI    BsaHI    BsaXI    BsiEI    BsiWI
    BsmBI    BspEI    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI    EcoRV
     FalI     FseI    FspAI    HaeII   HincII     HpaI     KasI     KpnI
     MfeI     MluI     MscI   MspA1I     NaeI     NarI     NcoI   NgoMIV
     NheI     NotI     NruI     PacI     PciI    PflMI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII    SacII     SalI    SanDI     SbfI    SexAI     SfiI
     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI
     StyI     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI
     ZraI
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