Restriction map for pF1KB7747
 (Linear) (Six-base) MAPSORT of: KIBB7747.seq  Check: 2758  from: 1  to: 1326



KIBB7747.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:02  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     355    1326 
   Size:        355     971
 
AflIII A'CryG_T

Cuts at:      0    1221    1235    1326 
   Size:       1221      14      91

  Fragments arranged by size: 

                1221      91      14
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1116    1326 
   Size:       1116     210
 
AlwNI CAG_nnn'CTG

Cuts at:      0     271     906    1326 
   Size:        271     635     420

  Fragments arranged by size: 

                 635     420     271
 
ApoI r'AATT_y

Cuts at:      0     483    1326 
   Size:        483     843
 
AvrII C'CTAG_G

Cuts at:      0     952    1326 
   Size:        952     374
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      36    1326 
   Size:         36    1290
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     277     311    1326 
   Size:        277      34    1015

  Fragments arranged by size: 

                1015     277      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     342    1326 
   Size:        342     984
 
BclI T'GATC_A

Cuts at:      0     968    1326 
   Size:        968     358
 
BlpI GC'TnA_GC

Cuts at:      0      77    1326 
   Size:         77    1249
 
BmgBI CAC'GTC

Cuts at:      0    1222    1236    1326 
   Size:       1222      14      90

  Fragments arranged by size: 

                1222      90      14
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     435    1326 
   Size:        435     891
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     583    1326 
   Size:        583     743
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     124     151     575    1026    1326 
   Size:        124      27     424     451     300

  Fragments arranged by size: 

                 451     424     300     124      27
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     652    1326 
   Size:        652     674
 
BsaHI Gr'CG_yC

Cuts at:      0    1156    1326 
   Size:       1156     170
 
BsaWI w'CCGG_w

Cuts at:      0     505    1326 
   Size:        505     821
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     736     766    1326 
   Size:        736      30     560

  Fragments arranged by size: 

                 736     560      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     303     436    1326 
   Size:        303     133     890

  Fragments arranged by size: 

                 890     303     133
 
BseYI C'CCAG_C

Cuts at:      0      28    1326 
   Size:         28    1298
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     166    1066    1289    1326 
   Size:        166     900     223      37

  Fragments arranged by size: 

                 900     223     166      37
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     385    1326 
   Size:        385     941
 
BsrBI CCG'CTC

Cuts at:      0     886    1326 
   Size:        886     440
 
BsrDI GCAATG_nn'

Cuts at:      0     243    1326 
   Size:        243    1083
 
BsrFI r'CCGG_y

Cuts at:      0      17    1326 
   Size:         17    1309
 
BssSI C'ACGA_G

Cuts at:      0     776    1326 
   Size:        776     550
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     819    1326 
   Size:        819     507
 
BstYI r'GATC_y

Cuts at:      0     808    1001    1326 
   Size:        808     193     325

  Fragments arranged by size: 

                 808     325     193
 
BtgI C'CryG_G

Cuts at:      0     344     846    1137    1326 
   Size:        344     502     291     189

  Fragments arranged by size: 

                 502     344     291     189
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1119    1154    1326 
   Size:       1119      35     172

  Fragments arranged by size: 

                1119     172      35
 
DraI TTT'AAA

Cuts at:      0     945    1326 
   Size:        945     381
 
DraIII CAC_nnn'GTG

Cuts at:      0    1267    1326 
   Size:       1267      59
 
EaeI y'GGCC_r

Cuts at:      0    1140    1326 
   Size:       1140     186
 
EarI CTCTTCn'nnn_

Cuts at:      0     103     655    1326 
   Size:        103     552     671

  Fragments arranged by size: 

                 671     552     103
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     355     583    1326 
   Size:        355     228     743

  Fragments arranged by size: 

                 743     355     228
 
EcoO109I rG'GnC_Cy

Cuts at:      0     949    1092    1326 
   Size:        949     143     234

  Fragments arranged by size: 

                 949     234     143
 
EcoRI G'AATT_C

Cuts at:      0     483    1326 
   Size:        483     843
 
EcoRV GAT'ATC

Cuts at:      0     396    1326 
   Size:        396     930
 
HaeII r_GCGC'y

Cuts at:      0     129    1326 
   Size:        129    1197
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     421     453     800     832     975     993    1007    1025 
   Size:        421      32     347      32     143      18      14      18

Cuts at:   1025    1102    1134    1326 
   Size:         77      32     192

  Fragments arranged by size: 

                 421     347     192     143      77      32      32      32
                  18      18      14
 
HindIII A'AGCT_T

Cuts at:      0     802    1326 
   Size:        802     524
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     592     836    1326 
   Size:        592     244     490

  Fragments arranged by size: 

                 592     490     244
 
MslI CAynn'nnrTG

Cuts at:      0     236    1187    1326 
   Size:        236     951     139

  Fragments arranged by size: 

                 951     236     139
 
MspA1I CmG'CkG

Cuts at:      0      32     268     532    1326 
   Size:         32     236     264     794

  Fragments arranged by size: 

                 794     264     236      32
 
NaeI GCC'GGC

Cuts at:      0      19    1326 
   Size:         19    1307
 
NgoMIV G'CCGG_C

Cuts at:      0      17    1326 
   Size:         17    1309
 
NspI r_CATG'y

Cuts at:      0     452    1326 
   Size:        452     874
 
PfoI T'CCnGG_A

Cuts at:      0     620     975    1326 
   Size:        620     355     351

  Fragments arranged by size: 

                 620     355     351
 
PpuMI rG'GwC_Cy

Cuts at:      0     949    1326 
   Size:        949     377
 
PvuII CAG'CTG

Cuts at:      0     268     532    1326 
   Size:        268     264     794

  Fragments arranged by size: 

                 794     268     264
 
SmlI C'TyrA_G

Cuts at:      0     103     130     590    1041    1326 
   Size:        103      27     460     451     285

  Fragments arranged by size: 

                 460     451     285     103      27
 
StuI AGG'CCT

Cuts at:      0    1279    1326 
   Size:       1279      47
 
StyI C'CwwG_G

Cuts at:      0     184     209     952    1326 
   Size:        184      25     743     374

  Fragments arranged by size: 

                 743     374     184      25
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1279    1326 
   Size:       1279      47
 
TatI w'GTAC_w

Cuts at:      0     668    1272    1326 
   Size:        668     604      54

  Fragments arranged by size: 

                 668     604      54
 
Tth111I GACn'n_nGTC

Cuts at:      0      40    1326 
   Size:         40    1286
 
XmnI GAAnn'nnTTC

Cuts at:      0    1168    1326 
   Size:       1168     158
 

 Enzymes that do cut:

     AcuI   AflIII     AhdI    AlwNI     ApoI    AvrII     BbsI     BcgI
    BciVI     BclI     BlpI    BmgBI     BmrI     BpmI    BpuEI     BsaI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsmBI    BsrBI
    BsrDI    BsrFI    BssSI    BstXI    BstYI     BtgI    CspCI     DraI
   DraIII     EaeI     EarI  Eco57MI EcoO109I    EcoRI    EcoRV    HaeII
    Hin4I  HindIII     MmeI     MslI   MspA1I     NaeI   NgoMIV     NspI
     PfoI    PpuMI    PvuII     SmlI     StuI     StyI    TaqII     TatI
  Tth111I     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI     BaeI    BamHI     BanI    BanII     BbeI    BbvCI    BfrBI
     BglI    BglII Bme1580I     BmtI     BplI   Bpu10I    BsaAI    BsaBI
    BsiEI  BsiHKAI    BsiWI     BsmI Bsp1286I    BspEI    BspHI    BspMI
    BsrGI   BssHII   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI
     BtsI     ClaI     DrdI     EagI     EciI  EcoICRI    EcoNI     FalI
     FseI     FspI    FspAI   HincII     HpaI     KasI     KpnI     MfeI
     MluI     MscI     NarI     NcoI     NdeI     NheI     NotI     NruI
     NsiI     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     SwaI     TstI     XbaI     XcmI     XhoI
     XmaI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com