Restriction map for pF1KB7730
##################################
# NO CUT ENZYMES (  107 enzymes) #
##################################

Acc65I      AccI        AclI        AcsI        AflII       
AflIII      AgeI        AhdI        Alw44I      AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AvrII       BalI        BbrPI       BcgI        BciVI       
BclI        BfrI        BglII       BlnI        BlpI        
Bpu1102I    BsaAI       BsaBI       BsaI        BsiWI       
Bsp1407I    BspDI       BspHI       BspLU11I    BspMI       
BsrGI       BsrI        BssSI       Bst1107I    BstBI       
BstXI       BstZ17I     Bsu36I      CelII       ClaI        
CpoI        DraI        DraIII      DrdI        Eam1105I    
Eco47III    Eco81I      EcoNI       EcoRI       EcoRV       
EcoT22I     FbaI        FseI        HindIII     KpnI        
MamI        MfeI        MluI        MluNI       MscI        
MunI        NdeI        NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PstI        PvuI        RcaI        RsrII       
SalI        SbfI        ScaI        SfaNI       SfuI        
SgrAI       SmlI        SnaBI       SpeI        Sse8387I    
SspBI       SspI        SwaI        TfiI        XbaI        
XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AatII         136  GACGTC               GACGT^C
AccIII       1345  TCCGGA               T^CCGGA
AspHI         459  GAGCTC               G[AT]GC[AT]^C
AviII          54  TGCGCA               TGC^GCA
BamHI        1175  GGATCC               G^GATCC
BbvCI         383  CCTCAGC              CCTCAGC(-5/-2)
BseAI        1345  TCCGGA               T^CCGGA
BsiHKAI       459  GAGCTC               G[AT]GC[AT]^C
BsmI           51  GAATGC               GAATGC(1/-1)
BspEI        1345  TCCGGA               T^CCGGA
BsrDI         947  GCAATG               GCAATG(2/0)
BssHII        202  GCGCGC               G^CGCGC
BstAPI        111  GCAGCTGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       1425  GGTGACC              G^GT[ACGT]ACC
BstPI        1425  GGTGACC              G^GT[ACGT]ACC
BstYI        1175  GGATCC               [GA]^GATC[CT]
DpnII        1176  GATC                 ^GATC
EcoO65I      1425  GGTGACC              G^GT[ACGT]ACC
EcoT14I      1433  CCATGG               C^C[AT][AT]GG
FspI           54  TGCGCA               TGC^GCA
HpaI         1144  GTTAAC               GTT^AAC
KspI          590  CCGCGG               CCGC^GG
MaeII         137  ACGT                 A^CGT
MboI         1176  GATC                 ^GATC
MflI         1175  GGATCC               [GA]^GATC[CT]
MroI         1345  TCCGGA               T^CCGGA
MseI         1145  TTAA                 T^TAA
MslI          660  CATGAAGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI         1433  CCATGG               C^CATGG
NdeII        1176  GATC                 ^GATC
NspI         1259  GCATGC               [GA]CATG^[CT]
PpuMI        1097  GGGACCC              [GA]G^G[AT]CC[CT]
SacI          459  GAGCTC               GAGCT^C
SacII         590  CCGCGG               CCGC^GG
Sau3AI       1176  GATC                 ^GATC
SexAI         638  ACCAGGT              A^CC[AT]GGT
SfiI          397  GGCCCTGGAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI         1259  GCATGC               GCATG^C
StuI          693  AGGCCT               AGG^CCT
StyI         1433  CCATGG               C^C[AT][AT]GG
Tru9I        1145  TTAA                 T^TAA
Tsp509I      1051  AATT                 ^AATT
XcmI          966  CCACAAGATGAATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII        1175  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   39 enzymes) #
###################################

AfaI          420  GTAC                 GT^AC
              636  GTAC                
AlwI         1176  GATCC                GGATC(4/5)
             1175  GGATC               
AspI          625  GACGGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1099  GACCCTGTC           
BanII         354  GAGCCC               G[GA]GC[CT]^C
              459  GAGCTC              
BbeI            9  GGCGCC               GGCGC^C
             1079  GGCGCC              
BbsI         1010  GAAGAC               GAAGAC(2/6)
              139  GTCTTC              
BfaI          554  CTAG                 C^TAG
              697  CTAG                
BglI          319  GCCGCTGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              398  GCCCTGGAGGC         
BpuAI        1010  GAAGAC               GAAGAC(2/6)
              139  GTCTTC              
BsiEI          41  CGGCCG               CG[GA][CT]^CG
             1019  CGGCCG              
BsmBI         207  CGTCTC               CGTCTC(1/5)
              572  CGTCTC              
BsrBI         188  CCGCTC               CCGCTC(-3/-3)
              441  GAGCGG              
DraII         396  AGGCCCT              [GA]G^G[ACGT]CC[CT]
             1097  GGGACCC             
EagI           41  CGGCCG               C^GGCCG
             1019  CGGCCG              
EclXI          41  CGGCCG               C^GGCCG
             1019  CGGCCG              
Eco52I         41  CGGCCG               C^GGCCG
             1019  CGGCCG              
EcoO109I      396  AGGCCCT              [GA]G^G[ACGT]CC[CT]
             1097  GGGACCC             
HaeII           9  GGCGCC               [GA]GCGC^[CT]
             1079  GGCGCC              
HincII        886  GTCAAC               GT[CT]^[GA]AC
             1144  GTTAAC              
HindII        886  GTCAAC               GT[CT]^[GA]AC
             1144  GTTAAC              
KasI            9  GGCGCC               G^GCGCC
             1079  GGCGCC              
MaeI          554  CTAG                 C^TAG
              697  CTAG                
MaeIII        645  GTCAC                ^GT[ACGT]AC
             1426  GTGAC               
NaeI          759  GCCGGC               GCC^GGC
             1033  GCCGGC              
NarI            9  GGCGCC               GG^CGCC
             1079  GGCGCC              
NgoMIV        759  GCCGGC               G^CCGGC
             1033  GCCGGC              
PflFI         625  GACGGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1099  GACCCTGTC           
PflMI         424  CCAGGGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1070  CCACCTTATGG         
RsaI          420  GTAC                 GT^AC
              636  GTAC                
SapI         1028  GAAGAGC              GCTCTTC(1/4)
             1215  GAAGAGC             
SfoI            9  GGCGCC               GGC^GCC
             1079  GGCGCC              
SmaI           78  CCCGGG               CCC^GGG
              276  CCCGGG              
TaqI          155  TCGA                 T^CGA
             1241  TCGA                
Tsp45I        645  GTCAC                ^GT[GC]AC
             1426  GTGAC               
Tth111I       625  GACGGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1099  GACCCTGTC           
TthHB8I       155  TCGA                 T^CGA
             1241  TCGA                
Van91I        424  CCAGGGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1070  CCACCTTATGG         
XmaCI          78  CCCGGG               C^CCGGG
              276  CCCGGG              
XmaI           78  CCCGGG               C^CCGGG
              276  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   68 enzymes) #
#########################################

AccII       AciI        AcyI        AluI        AlwNI       
AvaI        AvaII       BanI        BbvI        BcnI        
BmyI        BpmI        BsaHI       BsaJI       BsaWI       
BseRI       BsgI        BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BsrFI       BssKI       
BstF5I      BstNI       BstUI       Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DsaI        EaeI        
EarI        EcoRII      FauI        Fnu4HI      FokI        
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MboII       MnlI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NlaIII      
NlaIV       PleI        PvuII       Sau96I      ScrFI       
SfcI        TseI        TspRI       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com